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Reviews in Endocrine and Metabolic Disorders

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01.06.2013

The use of mass spectrometry to improve the diagnosis and the management of the HPA axis

verfasst von: Phillip J. Monaghan, Brian G. Keevil, Peter J. Trainer

Erschienen in: Reviews in Endocrine and Metabolic Disorders | Ausgabe 2/2013

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Abstract

Liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) is becoming a standard analytical tool in the clinical laboratory for the measurement of small molecules, including steroid hormones. Endocrinologists are coming to acknowledge the superior quality of measurement that is achievable by LC-MS/MS through the enhanced analytical specificity and high sensitivity that this technique offers over conventional immunoassay (IA) methodologies. Additionally, LC-MS/MS overcomes many of the problems encountered in immunoassays, such as anti-reagent antibody interferences and cross-reactivity with structurally related compounds. The potential benefits of applying LC-MS/MS for the assessment of the hypothalamic-pituitary-adrenal (HPA) axis are beginning to be realised. This review critically evaluates recent developments in the application of LC-MS/MS for measurement of glucocorticoids and mineralocorticoids towards the diagnosis and management of HPA axis disorders and aims to address the current unmet need in this expanding field of endocrinology for which future studies into the potential applications of LC-MS/MS should be directed.

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Titel: The use of mass spectrometry to improve the diagnosis and the management of the HPA axis
verfasst von: Phillip J. Monaghan
Brian G. Keevil
Peter J. Trainer
Publikationsdatum: 01.06.2013
Verlag: Springer US
Erschienen in: Reviews in Endocrine and Metabolic Disorders / Ausgabe 2/2013
Print ISSN: 1389-9155
Elektronische ISSN: 1573-2606
DOI: https://doi.org/10.1007/s11154-013-9240-1

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