Erschienen in:
01.07.2010
Transcriptional target-based expression cloning of immunoregulatory molecules
verfasst von:
Rebecca L. Lamason, Stefanie M. Lew, Joel L. Pomerantz
Erschienen in:
Immunologic Research
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Ausgabe 1-3/2010
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Abstract
Immunologic research has benefited tremendously from expression-cloning strategies designed to isolate genes responsible for a wide variety of immunomodulatory activities, including cytokines, receptors, signaling proteins, and transcription factors. Here, we discuss the use of expression-cloning strategies that have been modified to detect cDNAs that influence gene expression as assayed by a transcriptional reporter. We summarize our experience with these screens, review important parameters, and discuss potential modifications.