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Forensic Toxicology

Erschienen in:

28.03.2022 | Case Report

Two DFSA cases involving midazolam clarified by the micro-segmental hair analyses

verfasst von: Duoqi Xu, Jiaojiao Ji, Ping Xiang, Hui Yan, Min Shen

Erschienen in: Forensic Toxicology | Ausgabe 2/2022

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Abstract

Purpose

In this study, an analytical procedure to identify trace amounts of drug in hair based on micro-segmental hair analysis was presented. The method also can be used to estimate the time of drug ingestion at daily precision by cutting a single hair into sub-millimeter segments which correspond to daily hair growth.

Methods

A method was established for efficient extraction of midazolam, one of the most frequently detected compound in drug-facilitated sexual assault (DFSA) cases, from each 0.4-mm hair segment and validated by ultra-high performance liquid chromatography coupled to tandem mass spectrometry (UPLC–MS/MS). Moreover, two DFSA cases were used to compare the micro-segmental hair analysis with the 1- cm segmental analysis method.

Results

The validation showed a lower limit of quantification of 0.5 pg/mm for midazolam, with intraday and interday accuracies (bias) from − 5.2 to 0.9%. The micro-segmental hair analysis method was applied to proximal 1-cm hair segment including hair bulbs in two DFSA cases. The micro-segmental hair analysis results in case 1 showed midazolam in the S15–S17 (5.6–6.8 mm from hair bulb) in a concentration range from 0.5 to 0.9 pg/mm, and the concentrations of midazolam in all hair micro-segments (0–1 cm from the scalp) in case 2 were from 0.5 to 2.0 pg/mm.

Conclusions

Comparison with the conventional method revealed that micro-segmental hair analysis may enhance the utility of hair drug testing and strengthen probative force in DFSA cases.

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Titel: Two DFSA cases involving midazolam clarified by the micro-segmental hair analyses
verfasst von: Duoqi Xu
Jiaojiao Ji
Ping Xiang
Hui Yan
Min Shen
Publikationsdatum: 28.03.2022
Verlag: Springer Nature Singapore
Erschienen in: Forensic Toxicology / Ausgabe 2/2022
Print ISSN: 1860-8965
Elektronische ISSN: 1860-8973
DOI: https://doi.org/10.1007/s11419-022-00621-1

Springer Medizin

Two DFSA cases involving midazolam clarified by the micro-segmental hair analyses

Abstract

Purpose

Methods

Results

Conclusions

Neu im Fachgebiet Rechtsmedizin

Molekularpathologische Untersuchungen im Wandel der Zeit

Vergleichende Pathologie in der onkologischen Forschung

Gastrointestinale Stromatumoren

Personalisierte Medizin in der Onkologie

Springer Medizin

Abstract

Purpose

Methods

Results

Conclusions

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