Background
Methods
Mosquito collection and rearing
Constraints of working with a family-based breeding design
Virus intrathoracic injections
RNA extractions
DENV analysis
Candidate selection
Accession number | Gene name | Transcriptomic study | Function | Tissue | Direction | Differentially expressed | Interfamily variation |
---|---|---|---|---|---|---|---|
AAEL001022 |
smp-30/regucalcin
| Ca2+ binding domain | Whole | Down | Yes | - | |
AAEL001156 |
CG5280
| - | Whole | Up | No | Yes | |
AAEL001392 |
defA-assoc
| Immunity | Whole, Carcass | Down/Up | Yes | - | |
AAEL002413 |
sphingomyelin
| [12] | Cellular membrane | Whole | Down | Yes | - |
AAEL002585 |
CLIPA11
| Serine protease | Whole, MG | Down | No | Yes | |
AAEL003619 |
-
| Na/Cl transporter | Whole | Down | Yes | - | |
AAEL003787 |
Nopo
| [12] | Zinc finger | Whole | Up | Yes | - |
AAEL004361 |
alpha-glucosidase
| [12] | Glycolysis | Whole | Down | Yes | - |
AAEL004861 |
degringolade
| Peroxisomal integral protein | Whole, Carcass | Up | No | Yes | |
AAEL005064 |
CLIPB5
| Serine protease | Whole | Down | No | Yes | |
AAEL005527 |
Nbr/mut-7
| miRNA maturation | Whole, SG | Down | No | Yes | |
AAEL006995 |
CG9657
| - | Whole | Down | No | No | |
AAEL007495 |
phosphoglycerate mutase
| Glycolysis | Whole, Carcass, MG | Down | No | No | |
AAEL007845 |
Rab5
| - | Receptor | - | - | Yes | - |
AAEL008013 |
Obp83b
| Odorant | Whole | Down | No | Yes | |
AAEL008108 |
GB76c
| Transmembrane signalling | Whole, Carcass, SG | Down | Yes | - | |
AAEL009317 |
Rab11
| [12] | GTPase, cellular trafficking | Whole | Up | No | No |
AAEL009602 |
Gdap1
| Mitochondrial membrane | Whole, Midgut | Down | No | No | |
AAEL009770 |
SUMOE2
| [12] | Sumoylation | Whole | Up | Yes | - |
AAEL011375 |
trypsin
| Serine protease | Whole, Carcass | Down | Yes | - | |
AAEL011566 |
-
| Adhesion | Whole, Carcass, MG | Down | Yes | - | |
AAEL011817 |
rent1
| [12] | mRNA decay | Whole | Down | No | Yes |
AAEL012089 |
xport-A
| Phototransduction | Whole, Carcass | Down | No | No | |
AAEL013712 |
Trypsin 5G1 precursor
| Serine protease | Whole, Carcass | Up | No | No | |
AAEL014108 | aquaporin | H2O Transporter | Whole, Carcass | Down | Yes | - |
Candidate gene expression
Statistical analysis
Results
DENV load classification
Immune genes and signaling
Apoptosis genes
Metabolism genes
Transporter and adhesion genes
Discussion
Immunity
Adhesion and intracellular transport
Metabolism
Caveats
Conclusions
Accession number | Gene name | Function | Direction of regulation |
---|---|---|---|
AAEL001022 |
smp-30/regucalcin
| Senescence marker protein, Ca2+ binding domain | Down |
AAEL001392 |
defensin A-assoc
| Immunity (TOLL, Imd) | Down/Up |
AAEL002413 |
sphingomyelin
| Component of cellular membranes, reshuffling of which is important for virus intake | Down |
AAEL003619 |
–
| Putative Na/Cl transporter | Down |
AAEL003787 |
Nopo
| Zinc finger, ubiquitination | Up |
AAEL004361 |
alpha-glucosidase
| Molecule that mediates glycolysis, essential for virus replication | Down |
AAEL007845 |
Rab5
| Involved in endocytic trafficking of DENV | – |
AAEL008108 |
GB76c
| Transmembrane signalling molecule, GTPase activity | Down |
AAEL009770 |
SUMOE2
| Involved in sumoylation, process that stabilizes NS5 for replication | Up |
AAEL011375 |
trypsin
| Serine protease; effects of this kind of proteins are well described in the midgut | Down |
AAEL011566 |
–
| Putative adhesion molecule | Down |
AAEL014108 | aquaporin | Water transporter | Down |