Background
Methods
Patients
Featuresa |
n
b
| Cases with higher mRNA levels of fecal KIAA0247 (>0.4897) | p-valuec | |
---|---|---|---|---|
Age (years) | ||||
≤ 66.0 | 27 | 11 (40.7%) | 0.874 | |
>66.0 | 28 | 12 (42.9%) | ||
Gender | ||||
Male | 37 | 14 (37.8%) | 0.290 | |
Female | 19 | 10 (52.6%) | ||
Dukes' stages | ||||
A+B | 28 | 14 (50.0%) | 0.210 | |
C+D | 27 | 9 (33.3%) | ||
Depth of invasion | ||||
T1+T2 | 24 | 14 (58.3%) | 0.053 | |
T3+T4 | 31 | 10 (32.3%) | ||
Lymphatic invasion | ||||
N0 | 32 | 15 (46.9%) | 0.568 | |
N1+N2+N3 | 23 | 9 (39.1%) | ||
Distant metastasis | ||||
No | 34 | 18 (52.9%) | 0.058 | |
Yes | 22 | 6 (27.3%) | ||
Tumor location | ||||
Right | 20 | 6 (30.0%) | ||
Left | 13 | 6 (46.2%) | 0.341 | |
Rectum | 19 | 10 (52.6%) | ||
CEA (ng/ml) | ||||
≤ 5 | 28 | 15 (53.6%) | 0.072 | |
>5 | 27 | 8 (29.6%) | ||
CA19-9 (U/ml) | ||||
<37 | 34 | 15 (44.1%) | 0.371 | |
≥ 37 | 19 | 6 (31.6%) | ||
Differentiation | ||||
Well/moderate | 47 | 22 (46.8%) | 0.065 | |
Poor | 5 | 0 (0%) |
Colonic cell lines and human multiple tissue cDNA
Total RNA extraction and reverse transcription reaction
qRT-PCR
Gene name | Reference | Primer sequence (5' to 3')a | Probe numberb |
---|---|---|---|
KIAA0247 | NM014734 | F: CTGCAGATTCAGAGAACAGTGAC | 82 |
R: CTCATGCTTCTTTCAACAGTGG | |||
Cyclin A2 | NM001237 | F: CCATACCTCAAGTATTTGCCATC | 67 |
(CCNA2) | R: TCCAGTCTTTCGTATTAATGATTCAG | ||
Cyclin B1 | NM031966 | F: CATGGTGCACTTTCCTCCTT | 18 |
(CCNB1) | R: AGGTAATGTTGTAGAGTTGGTGTCC | ||
Cyclin B2 | NM004701 | F: GCATTATCATCCTTCTAAGGTAGCA | 4 |
(CCNB2) | R: TGTAATACTGCTGCTTTAAGTTCCA |
Lentivirus-mediated RNA interference (RNAi) and overexpression of KIAA0247
Cell cycle analysis by flow cytometry
Immunodetection of KIAA0247
Statistical analysis
Results
Expression of KIAA0247 in multiple gastrointestinal tissues and colonic cell lines
Relationship of fecal KIAA0247 expression with clinical features of CRC patients
Featuresa |
n
b
| Kendall's tau-b | p-value |
---|---|---|---|
Age (years) | 55 | 0.020 | 0.836 |
CEA (ng/ml) | 55 | -0.076 | 0.430 |
CA19-9 (U/ml) | 53 | -0.076 | 0.440 |
Tumor size (cm) | 51 | -0.202 | 0.047 |