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Knee Surgery, Sports Traumatology, Arthroscopy

Erschienen in:

01.11.2014 | Experimental Study

Characterization of age-related changes of tendon stem cells from adult human tendons

verfasst von: Laura Ruzzini, Franca Abbruzzese, Alberto Rainer, Umile Giuseppe Longo, Marcella Trombetta, Nicola Maffulli, Vincenzo Denaro

Erschienen in: Knee Surgery, Sports Traumatology, Arthroscopy | Ausgabe 11/2014

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Abstract

Purpose

The present study evaluated the presence of stem cells in hamstring tendons from adult subjects of different ages. The aim was to isolate, characterize and expand these cells in vitro, and to evaluate whether cell activities are influenced by age.

Methods

Tendon stem cells (TSCs) were isolated through magnetic sorting from the hamstring tendons of six patients. TSC percentage, morphology and clonogenic potential were evaluated, as well as the expression of specific surface markers. TSC multi-potency was also investigated as a function of age, and quantitative polimerase chain reaction was used to evaluate gene expression of TSCs cultured in suitable differentiating media.

Results

The presence of easily harvestable stem cell population within adult human hamstring tendons was demonstrated. These cells exhibit features such as clonogenicity, multi-potency and mesenchymal stem cells markers expression. The age-related variations in human TSCs affect the number of isolated cells and their self-renewal potential, while multi-potency assays are not influenced by tendon ageing, even though cells from younger individuals expressed higher levels of osteogenic and adipogenic genes, while chondrogenic genes were highly expressed in cells from older individuals.

Conclusions

These results may open new opportunities to study TSCs to better understand tendon physiology, healing and pathological processes such as tendinopathy and degenerative age-related changes opening new frontiers in the management of tendinopathy and tendon ruptures.

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Titel: Characterization of age-related changes of tendon stem cells from adult human tendons
verfasst von: Laura Ruzzini
Franca Abbruzzese
Alberto Rainer
Umile Giuseppe Longo
Marcella Trombetta
Nicola Maffulli
Vincenzo Denaro
Publikationsdatum: 01.11.2014
Verlag: Springer Berlin Heidelberg
Erschienen in: Knee Surgery, Sports Traumatology, Arthroscopy / Ausgabe 11/2014
Print ISSN: 0942-2056
Elektronische ISSN: 1433-7347
DOI: https://doi.org/10.1007/s00167-013-2457-4

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Characterization of age-related changes of tendon stem cells from adult human tendons