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01.12.2018 | Research | Ausgabe 1/2018 Open Access

Critical Care 1/2018

Clinical course, treatment and outcome of Pneumocystis pneumonia in immunocompromised adults: a retrospective analysis over 17 years

Zeitschrift:
Critical Care > Ausgabe 1/2018
Autoren:
Julius J. Schmidt, Catherina Lueck, Stefan Ziesing, Matthias Stoll, Hermann Haller, Jens Gottlieb, Matthias Eder, Tobias Welte, Marius M. Hoeper, André Scherag, Sascha David
Wichtige Hinweise

Electronic supplementary material

The online version of this article (https://​doi.​org/​10.​1186/​s13054-018-2221-8) contains supplementary material, which is available to authorized users.
Julius J Schmidt and Catherina Lueck contributed equally to this work and are co-first authors.
André Scherag and Sascha David contributed equally to this work and are co-last authors.
Abbreviations
AUC
Area under the curve
BAL
Broncho-alveolar lavage
BMI
Body mass index
BW
Bodyweight
cART
Combination antiretroviral therapies
CI
Confidence interval
CRRT
Continuous renal replacement therapy
CVID
Common variable immune-deficiency
ECMO
Extracorporeal membrane oxygenation
eGFR
Estimated glomerular filtration rate
HIV
Human immunodeficiency virus
HR
Hazard ratio
IS
Immunosuppressive
LDH
Lactate dehydrogenase
OR
Odds ratio
PcP
Pneumocystis pneumonia
RD
Rheumatic disease
ROC
Receiver operating characteristic curve
SOT
Solid organ transplantation
TMP-SMX
Trimethoprim-sulfamethoxazole

Background

Pneumocystis pneumonia (PcP) is a severe disease with high morbidity and mortality, which almost exclusively affects immunocompromised patients. PcP has long been known for its high prevalence among human immunodeficiency virus (HIV)-positive patients [ 1]. Since the implementation of combination antiretroviral therapies (cART) and chemoprophylaxis its incidence has been continuously decreasing [ 2]. Nowadays, most patients with HIV-associated PcP are treatment-naïve with very low CD4 cell counts; some of these patients do not know that they are HIV positive until they attend hospital [ 3]. On the other hand, PcP is also frequently diagnosed in non-HIV-positive patients as immunosuppressive regimens are being increasingly used in a wide range of patient populations. Consistently, the incidence of PcP in non-HIV-positive patients is increasing [ 2].
Pneumocystis infections have first been described in preterm infants following World War II [ 4] and in patients with malignancies in the late 1960s [ 5], more than a decade before the HIV epidemic emerged. Defects in cell-mediated immunity and use of glucocorticoids are among the strongest risk factors for the development of PcP [ 6, 7].
The epidemiology of PcP has been described in several retrospective studies [ 2, 8]. A study from the Mayo Clinic in 116 non-HIV-positive patients found that most frequent underlying diseases were hematological malignancies (30%), organ transplantation (25%), autoimmune disease (22%), solid tumors (13%) and other reasons [ 6]. Although, most of these reports were published more than a decade ago and might not reflect the current epidemiological situation, a report from France was published a few years ago [ 9]. Roux et al. reported overall mortality of 17.4%, which was significantly higher in non-HIV-positive patients (27%) than in HIV-positive patients (4%) [ 9]. A few parameters such as age, prior episode of PcP, low CD4 cell count, lactate dehydrogenase (LDH), and coinfections have been reported to predict unfavorable outcome in patients with HIV infection [ 1012]. Reports on outcome predictors in non-HIV-positive patients are sparse.
Based on the high burden of PcP and the likelihood of unfavorable outcome particularly in non-HIV-positive patients, chemoprophylaxis with trimethoprim-sulfamethoxazole (TMP-SMX) is recommended in high-risk populations [ 13]. TMP-SMX is also the treatment of choice for PcP. Adjunctive corticosteroid therapy is recommended in HIV-positive patients with severe respiratory failure [ 14, 15]. However, while beneficial outcomes of higher dosage of corticosteroids in HIV-positive patients during PcP are reported [ 16], the outcome of using corticosteroids in non-HIV-positive patients is not clear [ 17].
We here report comprehensive epidemiological, clinical, laboratory, therapeutic and outcome data on 240 cases of PcP, including a high percentage of non-HIV-positive patients, in a tertiary care center over the last 17 years. Additionally, we aimed at identifying predictors of outcome.

Methods

Study design and population

We performed a retrospective, single-center cross-sectional analysis of all patients with a positive finding of Pneumocystis jirovecii on direct immunofluorescence testing or detection by Diff-Quick staining in the bronchial washing fluid or broncho-alveolar lavage (BAL) fluid, from January 2000 to June 2017. Our hospital is a university tertiary care center with approximately 1500 beds. Written informed consent was waived by the ethics committee due to the anonymized retrospective nature of the analysis.
All bronchial washing fluids or BAL samples from patients clinically suspected to have P. jirovecii pneumonia were evaluated within the study period. For every patient, clinical data on demographic characteristics, underlying disease, status of immune competence, treatment regimens of immunosuppression, PcP therapy regimen and mortality, were gathered in the study database. Date of diagnosis was defined as the date of microbiological confirmation. P. jirovecii direct immunofluorescence was performed using the Monofluo kit P. carinii (BioRad Laboratories, years 2000 to 2016) or the Detect IF Pneumocystis carinii kit (Axis-Shield Diagnostics Ltd., year 2017). Diff Quick staining was performed using the stain sets provided by Dade Behring or Siemens AG, respectively.

Statistical analysis

Standard descriptive statistics were used to summarize the data (e.g. continuous: mean ± standard deviation/count: absolute and relative frequencies/time-to-event: Kaplan–Meier estimator). To identify predictors of in-hospital mortality or survival we applied logistic and Cox proportional hazards models. Consequently, both odds ratio (OR) and hazard ratio (HR) estimates were reported. To obtain multivariable adjusted estimates, all main effects with univariate p values less than or equal to 0.15 were investigated simultaneously. In addition, confidence intervals (CI) were calculated with coverage of 95%. Finally, we also created receiver operating characteristics (ROC) curves for in-hospital death, which were summarized by area under the curve (AUC) estimates. ROC analyses were based on an increasingly complex (leave one out) logistic regression model with in-hospital mortality as the predicted outcome and the following predictors, which were all entered linearly: LDH alone, LDH + age, LDH + age + body mass index (BMI), and for LDH + age + BMI + estimated glomerular filtration rate (eGFR). All reported p values are nominal and two-sided. In this explorative study, we applied a significance level of α = 0.05 (two-sided). All statistical analyses were done using GraphPad Prism 5.0 (La Jolla, CA, USA) or R 3.4.2.

Results

Description of study population and etiology of immunosuppression

During the observation period, 52,364 BAL or bronchial washing fluid samples were investigated. A clinical suspicion of P. jirovecii infection was raised in 3652 of these specimens of which 252 (i.e. 6.9%) were microbiologically confirmed by immunofluorescence as positive for PcP. There were ultimately 240 patients enrolled into the study (12 were excluded due to incomplete data sets) (Fig.  1). In the overall population, 67 patients were female (27.9%) and the average age was 45 ± 15 years. There were 125 patients (52%) with HIV infections; 39 (16.3%) had undergone solid organ transplant and 38 (15.8%) had undergone chemotherapy due to hematologic or oncologic malignancies: 28 patients (11.7%) received immunosuppressive therapy for rheumatoid autoimmune diseases (IS/RD) and 10 patients (4.2%) had other underlying diseases (miscellaneous (MISC)), e. g. common variable immune-deficiency (CVID) (Table  1). Five patients could be classified into multiple categories. After case reevaluation, patients were classified to the most recent active disease prior to the PcP event.
Table 1
Outcome and characteristics of the investigated study population of patients with PcP
 
Overall
240 (100%)
HIV
125 (52%)
SOT
39 (16.3%)
ChTx
38 (15.8%)
IS/RD
28 (11.7%)
MISC
10 (4.2%)
Age (years)
44.8 ± 14.6
41 ± 10.6
49.5 ± 13.9 ***
49.7 ± 17.2 ***
51.2 ± 17.6 ***
37.3 ± 24.5
Women
67 (27.9%)
18 (14.4%)
11 (28.2%)
17 (44.7%) ♦♦♦
15 (53.6%) ♦♦♦
6 (60%) ♦♦
ICU admission
100 (41.7%)
31 (24.8%)
19 (48.7%) ♦♦
27 (71.1%) ♦♦♦
16 (57.1%) ♦♦
7 (70%) ♦♦
Mechanical ventilation
88 (36.7%)
26 (22.4%)
17 (43.6%)
21 (55.3%) ♦♦♦
16 (57.1%) ♦♦♦
6 (60%)
CRRT
39 (16.3%)
4 (3.2%)
19 (48.7%) ♦♦♦
6 (15.8%)
7 (25%) ♦♦♦
3 (30%) ♦♦
ECMO/ECLA treatment
11 (4.6%)
5 (4%)
3 (7.7%)
2 (5.3%)
1 (3.6%)
0 (0%)
in-hospital mortality (%)
61 (25.4%)
16 (12.8%)
15 (38.5%) ♦♦
17 (44.7%) ♦♦♦
10 (35.7%) ♦♦
3 (30%)
TMP-SMX prophylaxis (%)
12 (5%)
6 (4.8%)
1 (2.6%)
5 (13.2%)
0 (0%)
0 (0%)
initial LDH (U/L)
502.1 ± 281.6
482.2 ± 247.2
482.5 ± 374.0
498.0 ± 236.4
627.3 ± 347.2 *
493.5 ± 160.5
Abbreviations: PcP Pneumocystis pneumonia, ICU intensive care unit, CRRT continuous renal replacement therapy, ECMO extracorporeal membrane oxygenation, ECLA extracorporeal lung assist, TMP-SMX trimethoprim-sulfamethoxazole, HIV human immunodeficiency virus, SOT solid organ transplantation, ChTx chemotherapy, IS/RD immunosuppression/rheumatic diseases. MISC miscellaneous
Difference compared to HIV-positive patients (Fisher’s exact test): p < 0.05, ♦♦ p < 0.01, ♦♦♦ p < 0.001
Difference compared to HIV-positive patients (Mann–Whitney U test): * p < 0.05, ** p < 0.01, *** p < 0.001

Clinical course of Pneumocystis pneumonia

The average cumulative incidence of PcP in our institution was 13 ± 5 cases per year, with a peak in the years 2005–2010 (Additional file 1: Figure S1A). Of the 240 patients with PcP, 41.7% were admitted to the intensive care unit (ICU), with 36.6% in need of mechanical ventilation, 16.3% in need of renal replacement therapy (RRT), and 4.5% in need of extracorporeal membrane oxygenation (ECMO) support (Fig.  2a). The overall in-hospital mortality was 25.4%. The mortality was 58% in patients requiring ICU treatment, 74.4% in patients requiring RRT and 81.8% in patients requiring ECMO support, and only 1.6% of patients died on regular (non-ICU) wards (Fig. 2b). Mortality during the observation time from 2000 to 2017 slightly fluctuated but did not trend toward an improvement in more recent years (Additional file 1: Figure S1B).
The underlying disease was associated with outcome (Table 1). The lowest mortality was observed in HIV-infected patients (12.8%); the respective mortality rates in the non-HIV group were 38.4% in solid organ transplant recipients, 30.0% in patients with rheumatic diseases and 44.7% in patients with hematologic-oncologic diseases, respectively (Additional file 1: Figure S1C). Figure 2c, d summarizes ICU admission and ICU mortality with regard to the etiology of the patients’ immunosuppressive disease. Of note, only 12 patients (5%) were receiving chemoprophylaxis with TMP-SMX at the time when PcP was diagnosed (Table 1).

Predictors of in-hospital mortality

Univariate regression analysis revealed that age, BMI, GFR and initial LDH were associated with death from PcP. Both logistic and cox multivariable regression analyses identified LDH as a predictor of unfavorable outcome in PcP (Table  2, adjusted OR 1.17 (95% CI 1.09–1.27) per 50 U/L, p < 0.0001, adjusted HR 1.07 (95% CI 1.04–1.10) per 50 U/L, p < 0.0001).
Table 2
Regression model results for in-hospital mortality and overall survival in 240 patients with PcP underlining the role of LDH
Patient characteristic (potential predictors) a
Logistic regression models for in-hospital mortality
Cox regression models for overall survival
Univariate models
Multivariable model b
Univariate models
Multivariable model c
Odds ratio
95% CI
p value
Odds ratio
95% CI
p value
Hazard ratio
95% CI
p value
Hazard ratio
95% CI
p value
LDH (50 U/L)
1.16
(1.10–1.24)
1.5 × 10 −6
1.17
(1.09–1.27)
3.7 × 10 −5
1.08
(1.06–1.11)
1.2 × 10 −9
1.07
(1.04–1.10)
3.2 × 10 − 5
Age (5 years)
1.28
(1.15–1.44)
1.9 × 10 − 5
1.66
(0.82–3.87)
0.205
1.14
(1.05–1.24)
0.001
1.33
(0.76–2.32)
0.311
Sex (ref. male)
1.23
(0.65–2.31)
0.515
     
1.30
(0.81–2.09)
0.285
     
BMI (kg/m 2)
1.14
(1.06–1.23)
1.5 × 10 −4
0.66
(0.33–1.33)
0.229
1.11
(1.05–1.17)
1.7 × 10 −4
0.65
(0.40–1.06)
0.082
Admission GFR (10 mL/min/1.73 m 2)
0.90
(0.84–0.97)
0.003
0.80
(0.60–1.07)
0.121
0.96
(0.91–1.01)
0.084
1.01
(0.80–1.28)
0.914
Prednisone equivalent dose (50 mg/day)
1.52
(1.13–2.13)
0.010
1.32
(0.96–1.85)
0.083
1.08
(0.95–1.23)
0.231
1.04
(0.89–1.21)
0.626
TMP (10 kg/day)
0.70
(0.42–1.16)
0.166
     
0.87
(0.60–1.27)
0.469
     
Abbreviations: PcP Pneumocystis pneumonia, LDH lactate dehydrogenase, BMI body mass index, TMP trimethoprim-sulfamethoxazole, CI confidence interval
aUnit for which effect was estimated or reference (ref.) in parenthesis
bMultiple logistic regression including all predictors that met p ≤ 0.15 in one of the univariate analyses for both in-hospital mortality and overall survival; note that we also included quadratic terms for the continuous predictors (results not reported)
cMultiple Cox regression including all predictors that met p ≤ 0.15 in one of the univariate analyses for both in-hospital mortality and overall survival; note that we also included quadratic terms for the continuous predictors (results not reported)
The initial LDH levels were significantly higher in later non-survivors (443 ± 214 vs. 673 ± 373 iU/L, p < 0.0001, Fig.  3a) and in patients that required admission to the ICU (411 ± 199 vs. 627 ± 328 iU/L, p < 0.0001, Fig. 3b). Stratification of patients into LDH quartiles showed increasing (in-hospital) mortality rates among those. Mortality ranged between 8% in the lowest and 49% in the highest LDH quartile (Fig. 3c). A ROC curve for in-hospital death had an estimated AUC of 0.724 (95% CI 0.65–0.80) ( p < 0.0001 for LDH alone, Additional file 1: Figure S2). Potential alternative cutoff values and their impact on usually reported statistics are summarized in Additional file 2: Table S1 for descriptive purposes. We used a cutoff of 496 iU/L for further analysis. Patients with initial LDH below 496 iU/L had lower mortality than the overall population (13.1 vs. 25.4%) and lower mortality than those with initial LDH > 496 iU/L (13.1 vs. 43.9%, p < 0.001, Fig. 3d, e).
The addition of other clinical quantitative variables to LDH in the AUC model such as age, BMI and eGFR did not improve the predictive value of LDH alone (Additional file 1: Figure S2, Additional file 2: Table S2) in the overall cohort. However, when ICU patients were analyzed separately, we observed that the addition of age as a variable increased the predictive performance of LDH alone from an AUC of 0.61 (95% CI 0.49–0.72) to an AUC of 0.71 (95% CI 0.60–0.81) ( p = 0.024). Of note, we did not detect any etiology subgroup that showed better performance in the ROC analysis (Additional file 1: Figure S3), nor did we detect temporal trends when applying the LDH prediction models in three strata (2000–2005, 2005–2010, 2010–2017) (Additional file 1: Figure S4).

Treatment patterns and outcome

There were 224/240 patients (93.3%) initially treated with the gold standard, TMP-SMX. The remaining 16 patients had alternative regimens with chemotherapeutics from the second line, mostly due to side effects and intolerance of TMP/SMX (e.g. atovaquone and/or pentamidine, Fig. 1). Among the 224 patients treated with TMP-SMX, 159 (71%) were dosed in the recommended range above 15 mg/kg bodyweight (BW) per day, while 65 patients (29%) received a dose lower than 15 mg/kg BW (Additional file 1: Figure S5A). Of note, these patients had a higher mortality risk (HR 1.80 (95% CI 1.10–3.44), p = 0.02). Solid organ transplant (SOT) recipients and patients with rheumatoid diseases were more likely to receive lower dosages (Additional file 1: Figure S5B). On the other side, we observed that under-dosed patients also had significantly lower eGFR (48.9 ± 43 ml/min vs. 104 ± 32 ml/min, p < 0.0001). Interestingly, the absolute dose of TMP-SMX was not different in survivors (16.9 ± 5.5 mg/kg) and non-survivors (15.6 ± 7.1 mg/kg, p = 0.93). Still, Kaplan–Meier survival curves revealed a significant difference when patients were grouped into those treated with < 15 mg/kg and those treated with ≥ 15 mg/kg TMP-SMX (Additional file 1: Figure S5C, p log-rang test = 0.02). However, TMP-SMX dose did not independently predict outcome in the multivariable regression analyses.

Discussion

Here we present the single-center experience with regard to epidemiology, treatment and outcome of PcP in a tertiary care center over a period of 17 years. A substantial group of our PcP cases (about 50%) were not related to HIV infections and these patients had a worse clinical course with higher ICU admission and mortality rates than patients with HIV-associated PcP. Three major non-HIV-positive groups were identified: (1) solid organ transplant recipients, (2) patients with malignancies and (3) patients with rheumatic diseases. PcP occurred almost exclusively in patients who did not receive chemoprophylaxis with TMP-SMX. Overall, about one quarter of all patients suffering from PcP did not survive the disease. In those who required intensive care (approximately 40% of all patients), the in-hospital mortality increased up to 58%. Based on the - in principle - reversible nature of PcP, almost no patients were denied intensive care in the event of acute respiratory failure, as the in-hospital mortality rate in non-ICU patients was 1.6%. Moreover, we found that the extent of initial LDH elevation was a predictor of in-hospital mortality, and that in univariate comparisons, in-hospital mortality was higher in patients whose TMP-SMX dose was below 15 mg/kg BW.
Non-HIV-positive populations at risk of PcP have been described before. The distribution across these risk groups varies widely (SOT 3–31%, hematologic diseases 26–39%, rheumatic diseases 11–25%), which is likely due to the clinical emphasis in predominantly monocentric studies [ 9, 1820]. Several centers have reported a decrease in the percentage of HIV-infected patients among all cases of PcP in the era of cART [ 19, 21]. We did not confirm this decrease, nor did Fillatre et al. [ 18].
Our data showed that the clinical course and outcome of PcP differed between HIV-infected and non-HIV-infected patients. In general, HIV-infected patients require fewer ICU admissions and have better overall survival than non-HIV-infected patients, which is in line with previous reports [ 9, 22]. The PcP mortality rate in HIV-positive patients was in the range of 1–15% [ 9, 18, 19] compared to 30–40% in HIV-negative patients [ 2325]. The better outcome of HIV-positive patients might be the consequence of (1) lower age (− 3.8 years in our cohort), (2) fewer co-morbidities, (3) the reversible nature of the immune defect upon anti-viral treatment strategies and hypothetically (4) greater awareness of PcP in HIV-positive individuals, where it is the most common AIDS-defining disease.
In mixed cohorts, ICU admission and mechanical ventilation occurred in 22–40% and 13–22% of patients, respectively [ 9, 18, 22]. Both events are frequently reported as negative predictors of survival. Nevertheless, newer data on ICU mortality in patients with PcP are scarce. Only one recent study reported relatively high ICU mortality in HIV-negative patients of 53% compared to 15% in HIV-positive patients [ 18]. Mortality in patients with invasive mechanical ventilation was 60% and 28%, respectively, in these two groups [ 22]. Compared to other reports [ 21, 26], we had a relatively high ICU admission rate of 25% in HIV-positive patients, which can in part be explained by the selective transfer of more patients with more progressive disease to our university hospital as a tertiary center.
The literature is inconclusive on outcome predictors, but on multivariable analysis, a large prospective study from France demonstrated a significant survival benefit in younger patients [ 9]. Although observed in HIV-positive patients before [ 27], here we report for the first time that in a mixed population of HIV-positive and non-HIV-positive patients the initial levels of serum LDH at hospital admission are not only useful in the diagnostic evaluation [ 28] but may also be an independent predictor of survival. This might potentially help the clinician in early identification of the sickest patients at high risk of unfavorable outcome. With regard to the specificity/sensitivity, the ideal LDH cutoff value to predict outcome has yet to be defined.
With regard to the actual treatment of PcP we found that a reduction in the recommended TMP-SMX dose below 15 mg/kg BW (for whatever reason) might be associated with higher mortality (13.1 vs. 55.8%). Given that this observation was not confirmed in the multivariable analysis, relevant confounders must be considered.
Our study has several limitations. This was a retrospective observational study based on the medical records of patients with PcP from a single institution. Consequently, causal claims cannot be made. The observation that under-dosing with TMP-SMX is associated with higher mortality might be confounded by a higher percentage of patients with renal impairment. More robust evidence from well-designed studies is needed to make firm conclusions or even to make implications about changes in standard PcP management.

Conclusions

In summary, PcP is a rare but potentially fatal disease in immunocompromised patients with diseases of different etiology. About 50% of cases were non-HIV-associated. Initial LDH levels - if validated by others - might be a useful predictor of in-hospital mortality, and TMP-SMX treatment doses in patients at high risk of death (e.g. ICU admission + LDH > 495 U/L) should probably not be reduced below 15 mg/kg BW.

Acknowledgments

none

Funding

No external funding was received to conduct this study. However, Dr David’s laboratory is supported by a grant from the German Research Foundation (DA1209/4–3).

Availability of data and materials

The datasets used and analyzed during the current study are available from the corresponding author on reasonable request.

Ethics approval and consent to participate

Written informed consent was waived by the local ethics committee at Hannover Medical School due to the anonymized retrospective nature of the analysis.

Consent for publication

Not applicable.

Competing interests

The authors declare that they have no competing interests.

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