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Inflammation Research
Erschienen in: Inflammation Research 12/2013

01.12.2013 | Original Research Paper

Docosahexaenoic acid reduces inflammation and joint destruction in mice with collagen-induced arthritis

verfasst von: Melissa V. Olson, Ying-Chun Liu, Bindi Dangi, J. Paul Zimmer, Norman Salem Jr., Julie M. Nauroth

Erschienen in: Inflammation Research | Ausgabe 12/2013

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Abstract

Objective

This study was designed to determine the anti-inflammatory activity of docosahexaenoic acid (DHA), alone and in combination with eicosapentaenoic acid (EPA), in a murine model of rheumatoid arthritis, collagen induced arthritis (CIA).

Methods

The CIA was induced in DBA/1OlaHsd mice by the injection of bovine type II collagen in Freunds’s complete adjuvant on days 0 and 21. Mice were fed modified diets containing DHA and/or EPA for 4 weeks prior to the initial collagen injection until study termination at day 45. The severity of CIA was assessed by measuring erythema, edema and mobility of the digits on the fore and hind paws, as well as histology. The level of serum anti-collagen antibodies was determined by ELISA. The ex vivo effects of DHA and/or EPA on splenocyte proliferation and cytokine production were evaluated by BrdU method and ELISA.

Results

Prophylactic treatment with DHA, and not DHA/EPA, significantly reduced arthritis severity and joint damage. Treatment with DHA also decreased anti-collagen (CII) antibodies in vivo, downregulated interleukin-1β, interferonγ and upregulated protective interleukin-10 ex vivo.

Conclusion

Prophylactic treatment with DHA was efficacious in a mouse model of rheumatoid arthritis and may be a useful intervention strategy against inflammatory arthritis.
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Metadaten
Titel
Docosahexaenoic acid reduces inflammation and joint destruction in mice with collagen-induced arthritis
verfasst von
Melissa V. Olson
Ying-Chun Liu
Bindi Dangi
J. Paul Zimmer
Norman Salem Jr.
Julie M. Nauroth
Publikationsdatum
01.12.2013
Verlag
Springer Basel
Erschienen in
Inflammation Research / Ausgabe 12/2013
Print ISSN: 1023-3830
Elektronische ISSN: 1420-908X
DOI
https://doi.org/10.1007/s00011-013-0658-4

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