Skip to main content
Hauptrubriken
CME Facharzt-Training Webinare Zeitschriften Bücher e.Medpedia Podcast
Service
Jobbörse Info & Hilfe
Fachgebiete
Anästhesiologie Allgemeinmedizin Arbeitsmedizin Augenheilkunde Chirurgie Dermatologie Gynäkologie und Geburtshilfe HNO Innere Medizin Kardiologie Neurologie Onkologie und Hämatologie Orthopädie und Unfallchirurgie Pädiatrie Pathologie Psychiatrie Radiologie Rechtsmedizin Urologie Zahnmedizin
Themen
Klimawandel und Gesundheit Neues aus dem Markt COVID-19 Praxis und Beruf Seltene Erkrankungen GOÄ & EBM Panorama
Sammlungen
Kasuistiken Algorithmen & Infografiken Blickdiagnosen Arthropedia FlapFinder (für Dermatochirurgen) Videos Kongressberichterstattung Medizin für Apothekerinnen und Apotheker Für Ärztinnen und Ärzte in Weiterbildung Für Medizinstudierende
Erweiterte Suche
Anmelden
nach oben
Inflammation Research
Erschienen in: Inflammation Research 12/2013

01.12.2013 | Original Research Paper

Paeoniflorin inhibits inflammatory responses in mice with allergic contact dermatitis by regulating the balance between inflammatory and anti-inflammatory cytokines

verfasst von: Chun Wang, Jun Yuan, Hua-xun Wu, Yan Chang, Qing-tong Wang, Yu-jing Wu, Li-hua Liu, Wei Wei

Erschienen in: Inflammation Research | Ausgabe 12/2013

Einloggen, um Zugang zu erhalten

Abstract

Objective

Paeoniflorin (Pae) was previously reported to inhibit inflammation in the skin of mice with allergic contact dermatitis (ACD); however, the mechanism remains unclear. The primary purpose of this study was to investigate the effect of Pae on the regulation of cytokine production in a murine model of ACD.

Methods

ACD was induced in the mice by repeated application of dinitrochlorobenzene (DNCB) to their skin. Cutaneous inflammation was evaluated by measuring ear swelling and by histological examination. The cytokine levels were measured by enzyme-linked immunosorbent assays.

Results

The results showed that topical application of DNCB caused obvious swelling and inflammatory cell infiltration. Treatment with Pae (70 or 140 mg/kg/d) significantly inhibited the cutaneous inflammation and decreased thymocyte proliferation in the mice with ACD. Additional data indicated that Pae increased interleukin-4 (IL-4) and IL-10 production but reduced IL-2 and IL-17 levels in the serum as well as in thymocyte and splenocyte culture supernatants. As expected, IL-2 and IL-17 levels in the serum displayed a significant positive correlation with the severity of skin inflammation. In contrast, IL-4 and IL-10 levels were negatively correlated with the inflammation.

Conclusions

The anti-inflammatory action of Pae in the murine model of ACD may be related to its regulation of an imbalanced cytokine production.
Anhänge
Nur mit Berechtigung zugänglich
Literatur
1.
Popov A, Mirkov I, Miljković D, et al. Contact allergic response to dinitrochlorobenzene (DNCB) in rats: insight from sensitization phase. Immunobiology. 2011;216:763–70.PubMedCrossRef
2.
Zhang EY, Chen AY, Zhu BT. Mechanism of dinitrochlorobenzene-induced dermatitis in mice: role of specific antibodies in pathogenesis. PLoS One. 2009;4:e7703.PubMedCrossRef
3.
Akhtar N, Satyam A, Anand V, et al. Dysregulation of T(H) type cytokines in the patients of Parthenium induced contact dermatitis. Clin Chim Acta. 2010;411:2024–8.PubMedCrossRef
4.
Harrington LE, Hatton RD, Mangan PR, et al. Interleukin 17-producing CD4+ effector T cells develop via a lineage distinct from the T helper type1and 2 lineages. Nat Immunol. 2005;6:1123–32.PubMedCrossRef
5.
Wang B, Zhuang L, Fujisawa H, et al. Enhanced epidermal Langerhans cell migration in IL-10 knockout mice. J Immunol. 1999;162:277–83.PubMed
6.
Wang WJ, Hao CF, Qu QL, et al. The deregulation of regulatory T cells on interleukin-17-producing T helper cells in patients with unexplained early recurrent miscarriage. Hum Reprod. 2010;25:2591–6.PubMedCrossRef
7.
Longhi MS, Liberal R, Holder B, et al. Inhibition of Interleukin-17 Promotes Differentiation of CD25(−) Cells Into Stable T Regulatory Cells in Patients With Autoimmune Hepatitis. Gastroenterology. 2012;142:1526-35.e6.PubMedCrossRef
8.
Zheng YQ, Wei W, Zhu L, Liu JX. Effects and mechanisms of Paeoniflorin, a bioactive glucoside from paeony root, on adjuvant arthritis in rats. Inflamm Res. 2007;56:182–8.PubMedCrossRef
9.
Li X, Shen J, Zhong Z, et al. Paeoniflorin: a monomer from traditional Chinese medical herb ameliorates Schistosoma japonicum egg-induced hepatic fibrosis in mice. J Parasitol. 2009;95:1520–4.PubMedCrossRef
10.
Lee JY, Kang HS, Park BE, et al. Inhibitory effects of Geijigajakyak-Tang on trinitrobenzene sulfonic acid -induced colitis. J Ethnopharmacol. 2009;126:244–51.PubMedCrossRef
11.
Min WQ, Wei Q, Li HY, et al. A clinical study of total glucosides paeony in the treatment of rheumatoid arthritis: a multi-center trial. Chin J Rheumatol. 2005;9:487–91.
12.
Zhang L, Dong Y, Sun Y, et al. Role of four major components in the effect of Si-Ni-San, a traditional Chinese prescription, against contact sensitivity in mice. J Pharm Pharmacol. 2006;58:1257–64.PubMedCrossRef
13.
Sun Y, Dong Y, Jiang HJ, et al. Dissection of the role of paeoniflorin in the traditional Chinese medicinal formula Si-Ni-San against contact dermatitis in mice. Life Sci. 2009;84:337–44.PubMedCrossRef
14.
Liu ZQ, Jiang ZH, Chan K, et al. Pharmacokinetic interaction of paeoniflorin and sinomenine: pharmacokinetic parameters and tissue distribution characteristics in rats and protein binding ability in vitro. J Pharmacol Sci. 2005;99:381–91.PubMedCrossRef
15.
Yuan XY, Liu W, Zhang P, et al. Effects and mechanisms of aloperine on 2, 4-dinitrofluorobenzene -induced allergic contact dermatitis in BALB/c mice. Eur J Pharmacol. 2010;629:147–52.PubMedCrossRef
16.
Germolec DR, Nyska A, Kashon M, et al. Extended histopathology in immunotoxicity testing: interlaboratory validation studies. Toxicol Sci. 2004;78:107–15.PubMedCrossRef
17.
Zhang LL, Wei W, Yan SX, et al. Therapeutic effects of glucosides of Cheanomeles speciosa on collagen-induced arthritis in mice. Acta Pharmacol Sin. 2004;25:1495–501.PubMed
18.
Liu K, Xu Q. Roxithromycin inhibits the effector phase of delayed-type hypersensitivity. Int Immunopharmacol. 2008;8:126–31.PubMedCrossRef
19.
Akhtar N, Verma KK, Sharma A. Study of pro- and anti-inflammatory cytokine profile in the patients with parthenium dermatitis. Contact Dermatitis. 2010;63:203–8.PubMedCrossRef
20.
Fontenot JD, Rasmussen JP, Gavin MA. Rudensky AY.A function for interleukin 2 in Foxp3- expressing regulatory T cells. Nat Immunol. 2005;6:1142–51.PubMedCrossRef
21.
Cork MJ, Keohane SG, Gawkrodger DJ, et al. Cytokine dermatosis: reactivation of eczema during interleukin-2 infusion. Br J Dermatol. 1997;136:644–5.PubMedCrossRef
22.
Xu H, DiIulio NA, Fairchild RL. T cell populations primed by hapten sensitization in contact sensitivity are distinguished by polarized patterns of cytokine production: interferon gamma- producing (Tc1) effector CD8+ T cells and interleukin (IL) 4/IL-10-producing (Th2) negative regulatory CD4+ T cells. Exp Med. 1996;183:1001–12.CrossRef
23.
Takeshita K, Yamasaki T, Akira S, et al. Essential role of MHC II-independent CD4+ T cells, IL-4 and STAT6 in contact hypersensitivity induced by fluorescein isothiocyanate in the mouse. Int Immunol. 2004;16(5):685–95.PubMedCrossRef
24.
Nakae S, Komiyama Y, Nambu A, et al. Antigen-specific T cell sensitization is impaired in IL-17- deficient mice, causing suppression of allergic cellular and humoral responses. Immunity. 2002;17:375–87.PubMedCrossRef
25.
He D, Wu L, Kim HK, et al. CD8+ IL-17-producing T cells are important in effector functions for the elicitation of contact hypersensitivity responses. J Immunol. 2006;177:6852–8.PubMed
26.
Antonysamy MA, Fanslow WC, Fu F, et al. Evidence for a role of IL-17 in organ allograft rejection: IL-17 promotes the functional differentiation of dendritic cell progenitors. J Immunol. 1999;162:577–84.PubMed
27.
Albanesi C, Cavani A, Girolomoni G. IL-17 is produced by nickel-specific T lymphocytes and regulates ICAM-1 expression and chemokine production in human keratinocytes: synergistic or antagonist effects with IFN-gamma and TNF-alpha. J Immunol. 1999;162:494–502.PubMed
28.
Albanesi C, Scarponi C, Cavani A, et al. Interleukin-17 is produced by both Th1 and Th2 lymphocytes, and modulates interferon-gamma- and interleukin-4 induced activation of human keratinocytes. J Invest Dermatol. 2000;115:81–7.PubMedCrossRef
29.
Asarch A, Barak O, Loo DS, Gottlieb AB. Th17 cells: a new therapeutic target in inflammatory dermatoses. J Dermatolog Treat. 2008;19:318–26.PubMedCrossRef
30.
He D, Wu L, Kim HK, et al. IL-17 and IFN-gamma mediate the elicitation of contact hypersensitivity responses by different mechanisms and both are required for optimal responses. J Immunol. 2009;183:1463–70.PubMedCrossRef
31.
Minang JT, Areström I, Zuber B, et al. Nickel-induced IL-10 down-regulates Th1- but not Th2-type cytokine responses to the contact allergen nickel. Clin Exp Immunol. 2006;143:494–502.PubMedCrossRef
32.
Zhou H, Bian D, Jiao X, et al. Paeoniflorin protects against lipopolysaccharide-induced acute lung injury in mice by alleviating inflammatory cell infiltration and microvascular permeability. Inflamm Res. 2011;60:981–90.PubMedCrossRef
33.
Wu H, Wei W, Song L, et al. Paeoniflorin induced immune tolerance of mesenteric lymph node lymphocytes via enhancing beta 2-adrenergic receptor desensitization in rats with adjuvant arthritis. Int Immunopharmacol. 2007;7:662–73.PubMedCrossRef
34.
Cao W, Zhang W, Liu J, et al. Paeoniflorin improves survival in LPS-challenged mice through the suppression of TNF-α and IL-1β release and augmentation of IL-10 production. Int Immunopharmacol. 2011;11:172–8.PubMedCrossRef
35.
Tsuboi H, Hossain K, Akhand AA, et al. Paeoniflorin induces apoptosis of lymphocytes through a redox-linked mechanism. J Cell Biochem. 2004;93:162–72.PubMedCrossRef
36.
Zhang L, Zhang S. Modulating Bcl-2 family proteins and caspase-3 in induction of apoptosis by paeoniflorin in human cervical cancer cells. Phytother Res. 2011;25:1551–7.PubMedCrossRef
37.
38.
Yin Y, Sun Y, Gu L, et al. Jaceosidin inhibits contact hypersensitivity in mice via down-regulating IFN-γ/STAT1/T-bet signaling in T cells. Eur J Pharmacol. 2011;651:205–11.PubMedCrossRef
39.
Rácz E, Kurek D, Kant M, et al. GATA3 expression is decreased in psoriasis and during epidermal regeneration; induction by narrow-band UVB and IL-4. PLoS One. 2011;6:e19806.PubMedCrossRef
40.
Weise C, Zhu Y, Ernst D, et al. Oral administration of Escherichia coli Nissle 1917 prevents allergen-induced dermatitis in mice. Exp Dermatol. 2011;20:805–9.PubMedCrossRef
41.
Martiniuk F, Lee DS, Gaspari A, et al. Expression of CD70 and the TH17 transcription factor ROR gammaT in human contact dermatitis. J Drugs Dermatol. 2008;7:956–60.PubMed
42.
Lin J, Xiao L, Ouyang G, et al. Total glucosides of paeony inhibits Th1/Th17 cells via decreasing dendritic cells activation in rheumatoid arthritis. Cell Immunol. 2012;280:156–63.PubMedCrossRef
43.
Wang K, Wu YG, Su J, et al. Total glucosides of paeony regulates JAK2/STAT3 activation and macrophage proliferation in diabetic rat kidneys. Am J Chin Med. 2012;40:521–36.PubMedCrossRef
44.
Zhao M, Liang GP, Tang MN, et al. Total glucosides of paeony induces regulatory CD4(+)CD25(+) T cells by increasing Foxp3 demethylation in lupus CD4(+) T cells. Clin Immunol. 2012;143:180–7.PubMedCrossRef
Metadaten
Titel
Paeoniflorin inhibits inflammatory responses in mice with allergic contact dermatitis by regulating the balance between inflammatory and anti-inflammatory cytokines
verfasst von
Chun Wang
Jun Yuan
Hua-xun Wu
Yan Chang
Qing-tong Wang
Yu-jing Wu
Li-hua Liu
Wei Wei
Publikationsdatum
01.12.2013
Verlag
Springer Basel
Erschienen in
Inflammation Research / Ausgabe 12/2013
Print ISSN: 1023-3830
Elektronische ISSN: 1420-908X
DOI
https://doi.org/10.1007/s00011-013-0662-8

Weitere Artikel der Ausgabe 12/2013

Inflammation Research 12/2013 Zur Ausgabe

Original Research Paper

Docosahexaenoic acid reduces inflammation and joint destruction in mice with collagen-induced arthritis

Original Research Paper

Tonic stimulation of the pharyngeal mucosa causes pain and a reversible increase of inflammatory mediators

Original Research Paper

The effect of 7-ketocholesterol and 25-hydroxycholesterol on the integrity of the human aortic endothelial and intestinal epithelial barriers

Original Research Paper

Effects of four antitussives on airway neurogenic inflammation in a guinea pig model of chronic cough induced by cigarette smoke exposure

Original Research Paper

Inflammatory mediators of systemic inflammation in neonatal sepsis

Original Research Paper

Direct comparison of laser Doppler flowmetry and laser Doppler imaging for assessment of experimentally-induced inflammation in human skin

Leitlinien kompakt für die Innere Medizin

Mit medbee Pocketcards sicher entscheiden.

Seit 2022 gehört die medbee GmbH zum Springer Medizin Verlag

Kostenlos registrieren

Neu im Fachgebiet Innere Medizin

Reizdarmsyndrom: Diäten wirksamer als Medikamente

29.04.2024 Reizdarmsyndrom Nachrichten

Bei Reizdarmsyndrom scheinen Diäten, wie etwa die FODMAP-arme oder die kohlenhydratreduzierte Ernährung, effektiver als eine medikamentöse Therapie zu sein. Das hat eine Studie aus Schweden ergeben, die die drei Therapieoptionen im direkten Vergleich analysierte.

Notfall-TEP der Hüfte ist auch bei 90-Jährigen machbar

26.04.2024 Hüft-TEP Nachrichten

Ob bei einer Notfalloperation nach Schenkelhalsfraktur eine Hemiarthroplastik oder eine totale Endoprothese (TEP) eingebaut wird, sollte nicht allein vom Alter der Patientinnen und Patienten abhängen. Auch über 90-Jährige können von der TEP profitieren.

Niedriger diastolischer Blutdruck erhöht Risiko für schwere kardiovaskuläre Komplikationen

25.04.2024 Hypotonie Nachrichten

Wenn unter einer medikamentösen Hochdrucktherapie der diastolische Blutdruck in den Keller geht, steigt das Risiko für schwere kardiovaskuläre Ereignisse: Darauf deutet eine Sekundäranalyse der SPRINT-Studie hin.

Bei schweren Reaktionen auf Insektenstiche empfiehlt sich eine spezifische Immuntherapie

25.04.2024 Allergien und Intoleranzreaktionen Nachrichten

Insektenstiche sind bei Erwachsenen die häufigsten Auslöser einer Anaphylaxie. Einen wirksamen Schutz vor schweren anaphylaktischen Reaktionen bietet die allergenspezifische Immuntherapie. Jedoch kommt sie noch viel zu selten zum Einsatz.

Update Innere Medizin

Bestellen Sie unseren Fach-Newsletter und bleiben Sie gut informiert.

Newsletter bestellen
Bildnachweise