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03.01.2021 | Original Article

The administration of hepatocyte growth factor prevents total parenteral nutrition-induced hepatocellular injury in a rat model

verfasst von: Makoto Matsukubo, Keisuke Yano, Tatsuru Kaji, Koshiro Sugita, Shun Onishi, Toshio Harumatsu, Ayaka Nagano, Mayu Matsui, Masakazu Murakami, Koji Yamada, Waka Yamada, Mitsuru Muto, Kotaro Kumagai, Akio Ido, Satoshi Ieiri

Erschienen in: Pediatric Surgery International | Ausgabe 3/2021

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Abstract

Purpose

Total parenteral nutrition (TPN) sometimes induces parenteral nutrition-associated liver disease (PNALD). Hepatocyte growth factor (HGF) acts as a potent hepatocyte mitogen anti-inflammatory and antioxidant actions. We aimed to evaluate the effect of HGF on PNALD in a rat model of TPN.

Methods

A catheter was placed in the right jugular vein for 7-day continuous TPN. All rats were divided into three groups: TPN alone (TPN group), TPN plus intravenous HGF at 0.3 mg/kg/day [TPN + HGF (low) group], and TPN plus HGF at 1.0 mg/kg/day [TPN + HGF (high) group]. On day 7, livers were harvested and the histology, inflammatory cytokines and apoptosis were evaluated.

Results

Histologically, lipid droplets were apparent in the TPN group, but decreased in the TPN + HGF (low) and TPN + HGF (high) groups. The histological nonalcoholic fatty liver disease activity scores in the TPN + HGF (low) and TPN + HGF (high) groups were significantly lower than that in the TPN group (p < 0.01). There were no significant differences in the inflammatory cytokine levels of the three groups. The caspase-9 expression levels in the TPN + HGF (low) and TPN + HGF (high) groups were significantly decreased in comparison to that in the control group (p < 0.05).

Conclusion

The intravenous administration of HGF attenuated hepatic steatosis induced by 7-day TPN dose dependently.

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Titel: The administration of hepatocyte growth factor prevents total parenteral nutrition-induced hepatocellular injury in a rat model
verfasst von: Makoto Matsukubo
Keisuke Yano
Tatsuru Kaji
Koshiro Sugita
Shun Onishi
Toshio Harumatsu
Ayaka Nagano
Mayu Matsui
Masakazu Murakami
Koji Yamada
Waka Yamada
Mitsuru Muto
Kotaro Kumagai
Akio Ido
Satoshi Ieiri
Publikationsdatum: 03.01.2021
Verlag: Springer Berlin Heidelberg
Erschienen in: Pediatric Surgery International / Ausgabe 3/2021
Print ISSN: 0179-0358
Elektronische ISSN: 1437-9813
DOI: https://doi.org/10.1007/s00383-020-04823-9

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The administration of hepatocyte growth factor prevents total parenteral nutrition-induced hepatocellular injury in a rat model