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01.02.2007 | EXPERIMENTAL

Effects of vaporized perfluorohexane and partial liquid ventilation on regional distribution of alveolar damage in experimental lung injury

verfasst von: Peter M. Spieth, Lilla Knels, Michael Kasper, André Domingues Quelhas, Bärbel Wiedemann, Amelie Lupp, Matthias Hübler, Antonio Gianella Neto, Thea Koch, Marcelo Gama de Abreu

Erschienen in: Intensive Care Medicine | Ausgabe 2/2007

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Abstract

Objective

To determine whether the patterns of distribution of histological effects of vaporized perfluorohexane (PFH) and partial liquid ventilation (PLV) differ significantly in acute lung injury.

Design and setting

Experimental study in an animal research laboratory.

Subjects

Eighteen pigs.

Interventions

After induction of acute lung injury by means of infusion of oleic acid animals were randomly assigned to PFH, PLV, or gas ventilation (GV) groups. Six hours thereafter animals were killed, and lung tissue samples were taken for analysis.

Measurements and results

Histopathological analysis revealed less damage with PFH than with GV or PLV in the nondependent and central regions. PFH and PLV showed less injury in the dependent regions than GV. GV and PFH were associated with less histological damage in the nondependent than the dependent regions, whereas PLV presented the opposite pattern. Morphometric analysis showed increased aeration in nondependent than dependent regions with PFH and GV. PLV led to more aeration in the periphery than in central areas.

Conclusions

PFH was associated with a more homogeneous attenuation of alveolar damage across the lungs, although this therapy had more pronounced effects in nondependent zones. PLV showed the opposite pattern, with more important reduction in alveolar damage in dependent lung regions. Interestingly, reduction in alveolar damage with PFH was as effective as with PLV in dependent zones. Our findings suggest that vaporized perfluorocarbon could be advantageous as adjunctive therapy in the treatment of acute lung injury.

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Titel: Effects of vaporized perfluorohexane and partial liquid ventilation on regional distribution of alveolar damage in experimental lung injury
verfasst von: Peter M. Spieth
Lilla Knels
Michael Kasper
André Domingues Quelhas
Bärbel Wiedemann
Amelie Lupp
Matthias Hübler
Antonio Gianella Neto
Thea Koch
Marcelo Gama de Abreu
Publikationsdatum: 01.02.2007
Verlag: Springer-Verlag
Erschienen in: Intensive Care Medicine / Ausgabe 2/2007
Print ISSN: 0342-4642
Elektronische ISSN: 1432-1238
DOI: https://doi.org/10.1007/s00134-006-0428-7

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Effects of vaporized perfluorohexane and partial liquid ventilation on regional distribution of alveolar damage in experimental lung injury