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Erschienen in:

01.09.2005 | Original

Endothelial oxidative stress induced by serum from patients with severe trauma hemorrhage

verfasst von: Christian Laplace, Olivier Huet, Eric Vicaut, Catherine Ract, Laurent Martin, Dan Benhamou, Jacques Duranteau

Erschienen in: Intensive Care Medicine | Ausgabe 9/2005

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Abstract

Objective

Shock induces oxidative stress by ischemia-reperfusion phenomenon. Endothelial cells are involved in the inflammatory response and oxidative stress responsible for microcirculation impairment and organ failure. We examined the potential of serum from patients to induce in vitro reactive oxygen species production by cultured human umbilical vein endothelial cells (HUVECs).

Patients

Three groups were compared: hemorrhagic shock trauma patients, isolated brain injured patients, and healthy volunteers.

Methods

In the hemorrhagic shock group we sought a correlation between reactive oxygen species production and severity of shock. Serum was separated and perfused in an in vitro model of perfused HUVECs. Ex vivo reactive oxygen species production was assessed by fluorescence microscopy using dichlorodihydrofluorescein, an intracellular dye oxidized by H₂O₂. Results are expressed in proportional change from baseline and normalized by protidemia to control for variation related to hemodilution.

Results

Reactive oxygen species production by endothelial cells exposed to serum from hemorrhagic shock patients (46.2±24.9%) was significantly greater than in those with brain injury (3.9±35.1%) and in healthy volunteers (−6.8±5.8%). In the hemorrhagic shock group dichlorodihydrofluorescein fluorescence was strongly correlated positively to Simplified Acute Physiology Score II and lactatemia and negatively to [HCO₃⁻].

Conclusions

Serum from trauma patients with hemorrhagic shock induces reactive oxygen species formation in naive endothelial cells which is correlated to shock severity.

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Titel: Endothelial oxidative stress induced by serum from patients with severe trauma hemorrhage
verfasst von: Christian Laplace
Olivier Huet
Eric Vicaut
Catherine Ract
Laurent Martin
Dan Benhamou
Jacques Duranteau
Publikationsdatum: 01.09.2005
Verlag: Springer-Verlag
Erschienen in: Intensive Care Medicine / Ausgabe 9/2005
Print ISSN: 0342-4642
Elektronische ISSN: 1432-1238
DOI: https://doi.org/10.1007/s00134-005-2737-7

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Endothelial oxidative stress induced by serum from patients with severe trauma hemorrhage