Morphometry by computerized three-dimensional reconstruction of the hypogastric plexus of a human fetus

The histological study of the plexus hypogastricus inferior (hypogastric plexus) of a human fetus does not permit the direct appreciation of its spatial configuration and its complicated relations. Developments in the field of computer science and three-dimensional (3D) reconstruction from serial histological sections have allowed a precise description of its morphometry and relations. The histological sections which were used came from the "Rouvière" collection of the Institute of Anatomy in Paris. A personal computer (IMAC) system of image analysis with reconstruction software was used. Serial pelvic histological sections were directly digitized from the slides. Image treatment and reconstruction were done with manual methods. The 3D reconstruction of the hypogastric plexus, the nerves, the pelvic skeleton and viscera were done. The hypogastric plexus and its topographic relations with the other organs were visualized and studied in three dimensions, and its morphometry was studied. The direct acquisition of the images from the slides allowed excellent high-quality digital images to be obtained. However, manual processing for the reconstruction was time-consuming. At first, the reconstruction of the various pelvic structures was done separately for each organ. Then the structures were visualized all together. Thus, the hypogastric plexus could be examined under various incidences with each organ. The virtual images obtained show new details of the topographic relations and improve knowledge of the precise innervation of the pelvic organs. The French version of this article is available in the form of electronic supplementary material and can be obtained by using the Springer Link server located at http://​dx.​doi.​org/​10.​1007/​s00276-002-0091-9.