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Neurological Sciences

Erschienen in:

01.12.2012 | Original Article

DAPT protects brain against cerebral ischemia by down-regulating the expression of Notch 1 and Nuclear factor kappa B in rats

verfasst von: Shuya Li, Xiangjian Zhang, Yongjun Wang, Hui Ji, Yuanyuan Du, Haichao Liu

Erschienen in: Neurological Sciences | Ausgabe 6/2012

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Abstract

Gamma-secretase inhibitor, N-[N-(3,5-difluorophenacetyl)-1-alanyl]-S-phenylglycine t-butyl ester (DAPT) suppresses the activation of Notch 1 signaling, which is recognized as the cell fate signaling and may participate in inflammatory processes together with NF-κB pathway that contributes to the brain damage after stroke. DAPT has important pharmacological roles in many diseases. However, little is known about the effect of DAPT on NF-κB during cerebral ischemia. This study investigated the time course expression of Notch 1 and the effects of DAPT on Notch 1 and NF-κB after MCAO. The results showed that Notch 1 signaling was up-regulated at the early stage after MCAO, DAPT down-regulated the expression of Notch 1 and NF-κB and protected brain from damage caused by MCAO. These results may indicate that the downregulation of Notch 1–NF-κB pathway after ischemia by administration of DAPT is a potential mechanism for its protection.

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Titel: DAPT protects brain against cerebral ischemia by down-regulating the expression of Notch 1 and Nuclear factor kappa B in rats
verfasst von: Shuya Li
Xiangjian Zhang
Yongjun Wang
Hui Ji
Yuanyuan Du
Haichao Liu
Publikationsdatum: 01.12.2012
Verlag: Springer Milan
Erschienen in: Neurological Sciences / Ausgabe 6/2012
Print ISSN: 1590-1874
Elektronische ISSN: 1590-3478
DOI: https://doi.org/10.1007/s10072-012-0948-6

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