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01.08.2011 | Original Paper

The survivin -31 snp in human colorectal cancer correlates with survivin splice variant expression and improved overall survival

verfasst von: Anna G. Antonacopoulou, Konstantina Floratou, Vasiliki Bravou, Anastasia Kottorou, Fotinos-Ioannis Dimitrakopoulos, Stella Marousi, Michalis Stavropoulos, Angelos K. Koutras, Chrisoula D. Scopa, Haralabos P. Kalofonos

Erschienen in: Cellular Oncology | Ausgabe 4/2011

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Abstract

Background

Survivin is involved in the regulation of cell division and survival, two key processes in cancer. The majority of studies on survivin in colorectal cancer (CRC) have focused on protein expression and less is known about the expression of survivin splicing variants or survivin gene polymorphisms in CRC. In the present study, the mRNA levels of the five known isoforms of survivin as well as survivin protein were assessed in matched normal and neoplastic colorectal tissue. Moreover, the 9386 C/T and -31 G/C polymorphisms were investigated.

Methods

Quantitative RT-PCR was used to assess mRNA levels in fresh/frozen tissue samples. Protein levels were immunohistochemically evaluated on formalin-fixed paraffin-embedded tissue sections. Individuals were genotyped using real time PCR

Results

Expression of all 5 survivin splice variants as well as survivin protein was elevated in colorectal carcinomas compared to normal tissue. Specific splice variant expression differentially correlated with clinicopathological parameters. Furthermore, both snps correlated with splice variant levels or their ratios in colorectal carcinomas while the -31 G/C snp may be related to CRC development and improved overall survival.

Conclusion

Our results support a role of survivin in colorectal carcinogenesis while the -31 G/C snp may constitute a marker of survival.

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Titel: The survivin -31 snp in human colorectal cancer correlates with survivin splice variant expression and improved overall survival
verfasst von: Anna G. Antonacopoulou
Konstantina Floratou
Vasiliki Bravou
Anastasia Kottorou
Fotinos-Ioannis Dimitrakopoulos
Stella Marousi
Michalis Stavropoulos
Angelos K. Koutras
Chrisoula D. Scopa
Haralabos P. Kalofonos
Publikationsdatum: 01.08.2011
Verlag: Springer Netherlands
Erschienen in: Cellular Oncology / Ausgabe 4/2011
Print ISSN: 2211-3428
Elektronische ISSN: 2211-3436
DOI: https://doi.org/10.1007/s13402-011-0038-4

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