Background
Methods
Tissue specimens
Histopathological preparation
Immunohistochemical study of ZO-1
Evaluation of immunohistochemistry staining
Immunofluorescence study of tight junction (ZO-1) in ECs co-cultured with PRBCs
Preparation of ECs
Preparation of PRBCs
Co-culture of ECs with PRBCs
Immunofluorescence staining of ZO-1
Evaluation of ZO-1
Statistical analysis
Results
Clinical and laboratory data of malaria patients
non-AKI (n = 10) | AKI (n = 10) | |
---|---|---|
Age | 27.9 ± 5.5 | 27.3 ± 4.1 |
Sex (M:F) | 9:1 | 6:4 |
Days of fever | 14.99 ± 0.57 | 19.88 ± 1.44 |
Parasitaemia (on admission) (/μl) | 379,813 ± 183,575 | 285,666 ± 90,400 |
Parasitaemia (last) (/μl) | 32,897 ± 31,971 | 54,121 ± 51,804 |
BUN (on admission) (mmol/L) | 14.46 ± 3.87 | 41.99 ± 4.54* |
BUN (last) (mmol/L) | 13.66 ± 2.54 | 32.53 ± 5.40* |
Cr (on admission) (μmol/L) | 124.74 ± 11.83 | 532.17 ± 70.73* |
Cr (last) (μmol/L) | 168.84 ± 30.62 | 432.44 ± 75.91* |
Glomerular changes in P. falciparum malaria
Evaluation of glomerular cells
Glomerular cell types in a glomerulus | Control (n = 10) | non-AKI (n = 10) | AKI (n = 10) |
---|---|---|---|
Mesangial cells | 11.91 ± 0.22 | 12.63 ± 0.28 | 19.28 ± 1.67*’** |
Endothelial cells | 66.24 ± 4.40 | 81.23 ± 2.49 | 88.13 ± 4.21*’** |
Podocytes | 12.04 ± 0.98 | 14.99 ± 0.57 | 19.88 ± 1.44*’** |
Total glomerular cells | 30.06 ± 5.72 | 36.02 ± 7.16 | 42.43 ± 7.24*’** |