Background
Methods/ design
Organotypic brain slices
Induction of “in vitro seizure”
Treatments
Recombinant proteins
Morphological study of vascularization and tight junctions
Immunostaining
Quantification of vascular density
Quantification of branching
Protein expression and activation
Cytokine array
Results
Organotypic cultures: a tool to decipher the mechanisms of BBB failure in epileptic disease
Roles and modifications of NVU cells in pathological conditions
OHCs are accessible for molecule screening in the culture medium
Cytokines | Others name | Inflammation | Angiogenesis | BBB disruption | References |
---|---|---|---|---|---|
CINC-1
| CXCL-1 or Gro-a |
Pro
|
Pro
|
/
| [34] |
[35] | |||||
CINC-2
| CXCL-3 or Gro-g |
Pro
|
Pro
|
/
| [35] |
[36] | |||||
CINC-3
| CXCL-2 or Gro-B |
Pro
|
Pro
|
/
| [35] |
[37] | |||||
CNTF
|
Pro
|
/
|
/
| [38] | |
Fractaline
| CX3CL-1 |
Pro
|
Pro
|
/
| [39] |
[40] | |||||
GM-CSF
|
Pro
|
Pro
|
Pro
| [40] | |
[41] | |||||
[42] | |||||
S-ICAM-1
| CD54 |
/
|
/
|
/
| |
IFN-g
|
Pro
|
Anti
|
Pro
| [43] | |
[41] | |||||
[44] | |||||
IL1-a
|
Pro
|
Pro
|
Pro
| [43] | |
[45] | |||||
[46] | |||||
IL1-b
|
Pro
|
Pro
|
Pro
| [45] | |
[47] | |||||
[48] | |||||
IL1-ra
|
Anti
|
Anti
|
/
| [49] | |
IL-2
|
Pro
|
Pro
|
Pro
| [50] | |
[51] | |||||
IL3
|
Pro
|
/
| [52] | ||
IL4
|
Pro
|
Anti
|
Pro
| [50] | |
[51] | |||||
[35] | |||||
IL6
|
Pro and anti
|
Pro
|
Pro
| [35] | |
[48] | |||||
[53] | |||||
IL10
|
Anti
|
Anti
|
/
| [54] | |
IP-10
| CXCL10 |
Pro
|
Anti
|
/
| [35] |
LIX
| CXCL5 |
Pro
|
Pro
|
/
| [35] |
L-selectine
| CD62L |
/
|
/
|
/
| |
MIG
| CXCL9 |
Pro
|
Anti
|
/
| [35] |
MIP-1a
| CCL3 |
Pro
|
/
|
/
| [35] |
MIP-3a
| CCL20 |
Pro
|
/
|
/
| [55] |
RANTES
| CCL5 |
Pro
|
Anti
|
Pro
| [56] |
TIMP-1
|
/
|
Anti
|
/
| [57] | |
TNF-a
|
Pro
|
Pro
|
Pro
| [48] | |
[35] | |||||
VEGF
|
/
|
Pro
|
Pro
| [58] |