Dear Editor,
The coronavirus disease 2019 (COVID-19) outbreak has been a severe challenge worldwide. Accumulating evidence reveals that in COVID-19 patients, inflammatory cell infiltration and cytokine storm are key factors leading to acute lung injury and poor prognosis [1]. Glucocorticoid (GC) was one of the anti-inflammatory medications widely used in critically ill patients. Numerous clinical studies have reported the efficacy of GC in the treatment of coronavirus pneumonia; however, the use of GC in the treatment of critical COVID-19 cases is still controversial [2, 3]. The main concern is that GC treatment may delay the clearance of virus. The current cohort study aimed to determine whether GC therapy would prolong the duration of SARS-CoV-2 RNA shedding and SARS-CoV-2 clearance.
Anzeige
This cohort study analyzed clinical data from 684 adult patients with SARS-CoV-2 infections confirmed through RT-PCR on throat swab samples collected between January and March 2020 from two hospitals in Wuhan, China. All patients received standard treatment including antiviral and oxygen therapy, and symptomatic support. The demographic, laboratory data at admission and discharge, GC treatment, and prognosis of the patients were collected. During the treatment, the throat swab and/or sputum and/or lower respiratory tract samples from confirmed patients were collected and tested by RT-PCR every 2 to 3 days. For the severe and critical patients, the interval between two tests was 4 to 5 days. To avoid false negative results, only patients with three continuously negative tests were considered that they have viral RNA clearance. Therefore, for the patients with negative RT-PCR result, two more samples were collected in the following 2 days, respectively. Among the 684 cases, 202 (29.5%) cases had viral RNA clearance within 14 days after illness onset and 210 (30.7%) cases had viral RNA clearance between 14 and 28 days, and 272 (39.8%) cases had viral RNA clearance over 28 days. There were no differences on the age, gender, and underlying diseases between different groups. The degree of decrease in CD4 T cell and B cell counts on admission was related with the prolonged viral RNA clearance (Table 1).
Table 1
Demographics and laboratory data at admission of patients infected with COVID-19 according to the time to SARS-CoV-2 RNA clearance
Overall (N = 684) | ≤ 14 days (N = 202) | 14–28 days (N = 210) | > 28 days (N = 272) | p value | |
|---|---|---|---|---|---|
Demographics, clinical characteristics | |||||
Age (years) | 61.0 [49.0, 70.0] | 62.0 [48.0, 71.0] | 60.5 [49.3, 70.0] | 61.5 [51.0, 70.3] | 0.733 |
Gender (%) | 0.841 | ||||
Male | 328 (48.0) | 98 (48.5) | 103 (49.3) | 127 (46.7) | |
Female | 355 (52.0) | 104 (51.5) | 106 (50.7) | 145 (53.3) | |
Clinical type (%) | 0.009 | ||||
Mild | 26 (3.9) | 16 (8.0) | 7 (3.4) | 3 (1.1) | |
General | 464 (69.3) | 140 (70.0) | 141 (69.1) | 183 (68.8) | |
Severe | 145 (21.6) | 36 (18.0) | 44 (21.6) | 65 (24.4) | |
Critical | 35 (5.2) | 8 (4.0) | 12 (5.9) | 15 (5.6) | |
Hypertension (%) | 235 (35.0) | 67 (34.0) | 70 (33.8) | 98 (36.6) | 0.778 |
CHD (%) | 75 (11.2) | 22 (11.2) | 25 (12.1) | 28 (10.4) | 0.855 |
CRF (%) | 16 (2.4) | 3 (1.5) | 7 (3.4) | 6 (2.2) | 0.467 |
DM (%) | 111 (16.5) | 30 (15.2) | 36 (17.5) | 45 (16.8) | 0.824 |
COPD (%) | 13 (1.9) | 3 (1.5) | 3 (1.4) | 7 (2.6) | 0.588 |
Cirrhosis (%) | 3 (0.4) | 0 (0.0) | 1 (0.5) | 2 (0.7) | 0.492 |
Stroke (%) | 45 (6.7) | 14 (7.1) | 14 (6.8) | 17 (6.3) | 0.943 |
Tumor (%) | 23 (3.4) | 5 (2.6) | 5 (2.4) | 13 (4.9) | 0.257 |
Inflammatory response, median (IQR) | |||||
CRP (mg/L) | 7.39 [0.50, 28.25] | 8.63 [1.04, 16.33] | 5.79 [0.50, 28.80] | 5.13 [0.50, 62.39] | 0.968 |
IL-6 (pg/mL) | 6.00 [2.25, 21.00] | 6.00 [3.00, 19.50] | 7.50 [2.00, 26.25] | 6.00 [3.00, 20.50] | 0.943 |
Fib (g/L) | 4.82 [3.68, 11.90] | 4.46 [3.58, 11.72] | 5.50 [3.65, 11.98] | 6.71 [4.08, 13.60] | 0.19 |
WBC (109/L) | 5.59 [4.46, 7.06] | 5.60 [4.47, 6.79] | 5.51 [4.52, 7.12] | 5.33 [4.38, 7.83] | 0.962 |
Neutrophil (109/L) | 3.33 [2.44, 4.76] | 3.33 [2.49, 4.51] | 3.31 [2.42, 4.85] | 3.67 [2.41, 5.83] | 0.618 |
Monocyte (109/L) | 0.46 [0.34, 0.60] | 0.45 [0.35, 0.59] | 0.46 [0.34, 0.61] | 0.47 [0.35, 0.58] | 0.970 |
Lymphocyte (109/L) | 1.27 [0.88, 1.81] | 1.29 [0.94, 1.86] | 1.29 [0.95, 1.73] | 1.00 [0.72, 1.67] | 0.204 |
PLT (109/L) | 203 [158, 249] | 209 [156, 267] | 196 [158, 241] | 189 [148, 244] | 0.209 |
Hb (g/L) | 121 [108, 133] | 123 [109, 133] | 121 [110, 131] | 121 [103, 131] | 0.609 |
CD3 (count/μL) | 8905 [474, 1212] | 916 [586, 1220] | 705 [423, 1224] | 497 [364, 684] | 0.115 |
CD4 (count/μL) | 478 [269, 672] | 554 [319, 733] | 324 [190, 588] | 275 [154, 404] | 0.048 |
CD8 (count/μL) | 248 [150, 374] | 291 [174, 400] | 229 [142, 367] | 167 [123, 211] | 0.112 |
NK (count/μL) | 167 [103, 259] | 142 [91.0, 231] | 206 [141, 332] | 154 [91.8, 215] | 0.115 |
B cell (count/μL) | 175 [104, 281] | 195 [131, 296] | 187 [91.0, 279] | 94.0 [72.8, 126] | 0.030 |
Organ function measurement, median (IQR) | |||||
ALT (U/L) | 27.0 [18.0, 41.0] | 26.0 [18.0, 40.5] | 29.0 [19.0, 43.0] | 27.0 [18.3, 38.3] | 0.679 |
AST (U/L) | 22.0 [16.0, 36.5] | 20.0 [15.0, 36.0] | 23.0 [17.0, 37.0] | 23.0 [19.0, 39.0] | 0.314 |
TBIL (μmol/L) | 11.2 [8.40, 14.0] | 11.3 [8.7, 14.0] | 11.0 [8.30, 13.0] | 11.1 [8.53, 15.8] | 0.731 |
DBIL (μmol/L) | 2.80 [2.10, 3.70] | 2.70 [2.20, 3.60] | 3.00 [1.92, 3.68] | 2.90 [1.90, 4.40] | 0.935 |
Creatinine (μmol/L) | 61.0 [50.0, 75.0] | 61.0 [50.0, 78.5] | 60.0 [50.2, 74.7] | 60.0 [50.2, 72.5] | 0.694 |
BUN (mmol/L) | 4.60 [3.60, 5.80] | 4.40 [3.50, 5.80] | 4.60 [3.70, 5.50] | 4.50 [3.70, 5.80] | 0.794 |
Lactate (mmol/L) | 1.10 [1.00, 1.35] | 1.00 [0.90, 1.30] | 1.10 [1.00, 1.30] | 1.10 [1.00, 1.60] | 0.395 |
Glucose (mmol/L) | 5.60 [5.00, 6.62] | 5.55 [5.00, 6.32] | 5.60 [5.03, 7.62] | 5.30 [4.90, 6.95] | 0.431 |
INR | 1.10 [1.00, 1.20] | 1.10 [1.00, 1.20] | 1.10 [1.00, 1.20] | 1.10 [1.10, 1.20] | 0.075 |
CK (U/L) | 66.5 [25.3, 110.5] | 67.0 [24.0, 107.5] | 62.0 [39.0, 135.0] | 63.0 [22.5, 105.5] | 0.530 |
BNP (pg/mL) | 67.9 [28.0, 152.3] | 67.9 [28.0, 243.0] | 62.0 [27.0, 126.0] | 97.0 [41.5, 128.8] | 0.490 |
NT-proBNP (pg/mL) | 745 [88.0, 1899] | 109 [67.0, 2679] | 1118 [769.5, 2237] | 745 [382, 859] | 0.532 |
GC treatment (%) | 103 (15.1) | 24 (11.9) | 32 (15.2) | 47 (17.3) | 0.266 |
Methylprednisolone (%) | 96 (14.0) | 24 (11.9) | 30 (14.3) | 42 (15.4) | 0.540 |
Dexamethasone (%) | 12 (1.8) | 2 (1.0) | 3 (1.4) | 7 (2.6) | 0.392 |
Hydrocortisone (%) | 1 (0.1) | 0 (0.0) | 0 (0.0) | 1 (0.4) | 0.468 |
Outcome | |||||
Hospital stay (days) | 25.0 [16.0, 38.0] | 21.0 [14.0, 28.0] | 24.0 [18.0, 32.0] | 37.0 [21.0, 47.0] | < 0.001 |
Total course (days) | 45.0 [33.0, 59.3] | 30.0 [19.5, 39.5] | 41.0 [32.0, 54.0] | 57.0 [47.0, 67.0] | < 0.001 |
Outcome (%) | 0.414 | ||||
Survival | 643 (96.8) | 191 (97.0) | 194 (95.6) | 258 (97.7) | |
Death | 21 (3.2) | 6 (3.0) | 9 (4.4) | 6 (2.3) | |
Since GC therapy was usually employed in critically ill patients, we analyzed the effect of GC therapy separately for patients with different severity. Patients were diagnosed as mild type, general type, severe type, and critical type according to the Chinese Recommendations for Diagnosis and Treatment of Novel Coronavirus (SARS-CoV-2) Infection (Trial 7th version) [4]. For the mild and general type patients, 30 (6.1%) cases received GC treatment and 460 (93.1%) cases did not. For the severe and critical type patients, 72 (40%) cases were in the GC group and 108 (60%) cases were in the non-GC group (Table 1). In this study, methylprednisolone was the most used glucocorticoid (Table 1) in a dose of 1–2 mg/(kg·day) for 3 to 5 days according to the disease severity [4]. The results show that GC therapy increased hospital stay days but had no effect on the virus clearance time (Table 2). For the severe and critical patients, the median viral RNA clearance time in the GC group was 26 days (IQR 17–42 days), while the viral RNA clearance time in the non-GC group was 25.5 days (IQR 13–39 days). In addition, the GC treatment had no effect on the peripheral lymphocyte counts, including CD4 T cells, CD8 T cells, NK cells, and B cells (Table 2).
Table 2
Effect of glucocorticoid on the outcome and inflammatory response on discharge of COVID-19 patients
Variables | Mild and general group | Severe and critical group | ||||
|---|---|---|---|---|---|---|
Non-GC group | GC group | p value | Non-GC group | GC group | p value | |
N | 460 | 30 | 108 | 72 | ||
Hospital stay (days) | 23.00 [16.00, 33.00] | 32.50 [23.25, 38.00] | 0.002 | 29.00 [17.00, 44.00] | 40.50 [31.75, 52.50] | < 0.001 |
Viral RNA clearance (days) | 22.00 [11.00, 35.00] | 23.50 [14.00, 34.25] | 0.737 | 25.50 [13.00, 39.00] | 26.00 [17.00, 42.00] | 0.471 |
Total course (days) | 43.00 [29.00, 57.00] | 41.00 [31.50, 47.75] | 0.816 | 49.00 [37.50, 63.50] | 49.00 [43.00, 63.00] | 0.341 |
Outcome (%) | 1 | 0.555 | ||||
Survival | 446 (99.8) | 30 (100.0) | 92 (90.2) | 62 (86.1) | ||
Death | 1 (0.2) | 0 (0.0) | 10 (9.8) | 10 (13.9) | ||
Inflammatory response, median (IQR) | ||||||
CRP (mg/L) | 2.93 [0.90, 10.00] | 4.15 [2.04, 10.50] | 0.378 | 3.19 [0.77, 21.20] | 4.59 [1.16, 13.15] | 0.457 |
IL-6 (pg/mL) | 3.00 [2.00, 7.00] | 5.00 [2.00, 12.50] | 0.253 | 16.00 [4.75, 36.00] | 9.50 [7.25, 32.00] | 0.986 |
Fib (g/L) | 3.58 [2.78, 4.84] | 3.96 [3.58, 11.43] | 0.227 | 3.53 [2.84, 14.39] | 3.90 [3.60, 10.90] | 0.275 |
WBC (109/L) | 5.55 [4.40, 6.55] | 5.20 [4.25, 6.08] | 0.568 | 5.93 [5.00, 7.50] | 6.80 [4.90, 8.55] | 0.485 |
Neutrophil (109/L) | 3.17 [2.49, 4.04] | 3.05 [2.25, 3.98] | 0.641 | 3.76 [2.85, 5.20] | 4.79 [3.09, 6.80] | 0.178 |
Monocyte (109/L) | 0.46 [0.36, 0.57] | 0.49 [0.41, 0.66] | 0.177 | 0.43 [0.35, 0.66] | 0.51 [0.40, 0.63] | 0.311 |
Lymphocyte (109/L) | 1.56 [1.25, 1.91] | 1.47 [1.23, 1.88] | 0.801 | 1.21 [0.97, 1.79] | 1.10 [0.85, 1.55] | 0.207 |
PLT (109/L) | 209.0 [173.0, 258.0] | 212.0 [183.8, 266.5] | 0.923 | 212.0 [151.0, 236.0] | 183.0 [144.0, 250.0] | 0.615 |
Hb (g/L) | 128.5 [117.25, 138.8] | 133.0 [127.0, 137.0] | 0.667 | 117.5 [102.5, 129.0] | 119.0 [100.0, 125.0] | 0.669 |
CD3 (count/μL) | 1144 [861, 1293] | 1002 [793, 1282] | 0.791 | 509 [162.0, 1026] | 683.5 [478.8, 1112] | 0.508 |
CD4 (count/μL) | 625.0 [514.0, 831.5] | 498.0 [385.0, 801.0] | 0.442 | 306.0 [65.3, 585.5] | 338.5 [257.5, 547.0] | 0.449 |
CD8 (count/μL) | 357.0 [246.0, 454.0] | 364.0 [248.5, 478.5] | 0.845 | 133.0 [72.3, 341.5] | 257.0 [190.3, 389.3] | 0.257 |
NK (count/μL) | 201.0 [128.0, 271.5] | 90.0 [55.0, 132.0] | 0.008 | 52.5 [40.0, 124.00] | 93.50 [62.5, 117.3] | 0.257 |
B cell (count/μL) | 171.0 [132.5, 265.0] | 248.0 [146.0, 328.5] | 0.493 | 68.5 [58.5, 126.8] | 115.0 [94.3, 156.0] | 0.299 |
The current multicenter cohort study demonstrates that GC therapy does not change viral clearance and peripheral lymphocyte counts in COVID-19 patients. However, well-designed and large-scale randomized controlled trials are needed to further confirm the results derived from this observational study.
Acknowledgements
We thank the help from Zheying Liu and Conglin Wang during the data collection, and thank Jie Fan (University of Pittsburgh, USA) for revising the manuscript.
Anzeige
Ethics approval and consent to participate
The study was approved by the Research Ethics Commission of General Hospital of Southern Theater Command of PLA. The requirement for informed consent was waived by the Ethics Commission.
Consent for publication
All authors reviewed the manuscript and approved the publication.
Competing interests
The authors declare that they have no competing interests.
Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
Publisher’s Note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.