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01.12.2017 | Research article | Ausgabe 1/2017 Open Access

BMC Cancer 1/2017

Long non-coding RNA XIST regulates PTEN expression by sponging miR-181a and promotes hepatocellular carcinoma progression

Zeitschrift:
BMC Cancer > Ausgabe 1/2017
Autoren:
Shuzhen Chang, Binhe Chen, Xiaoyan Wang, Keqin Wu, Yuqiu Sun
Wichtige Hinweise

Electronic supplementary material

The online version of this article (doi:10.​1186/​s12885-017-3216-6) contains supplementary material, which is available to authorized users.
Shuzhen Chang and Binhe Chen contributed equally to this work.

Abstract

Background

Tumor metastasis often occurs in hepatocellular carcinoma (HCC) and influences the patient’s prognosis, and microRNAs are reported to play key roles in tumor metastasis. This study was conducted to explore the effect of microRNAs on HCC metastasis.

Methods

The levels of miR-181a in HCC tissues, adjacent tissues, metastatic HCC tissues, and non-metastatic HCC tissues at different stages were determined by qRT-PCR. Effect of miR-181a on the proliferation, invasion, and metastasis of HCC cells was estimated by cell counting kits-8 (CCK-8), wound-healing, and Transwell assays. Software analysis and luciferase assays were used to explore the target gene of miR-181a.

Results

MiR-181a was up-regulated in HCC tissues and its expression level in metastatic HCC tissues was much higher than in non-metastasis samples. PTEN was found to be a target gene of miR-181a. MiR-181a had multiple binding sites with the long non-coding RNA (lncRNA) XIST. The regulation of miR-181a on PTEN was mediated by lncRNA XIST. The proliferation and invasion of cells with siXIST were significantly enhanced compared with those of control cells, while knockdown of miR-181a abolished the enhancing effects.

Conclusions

MiR-181a can promote HCC metastasis by targeting PTEN, which is regulated by lncRNA XIST.
Zusatzmaterial
Additional file 1: Table S1. Primers used for quantitative real-time PCR. (DOCX 15 kb)
12885_2017_3216_MOESM1_ESM.docx
Additional file 2: Table S2. Sequences including the siRNA and the scramble sequence used in transfection assay. (DOCX 15 kb)
12885_2017_3216_MOESM2_ESM.docx
Additional file 3: Figure S1. (A) Relative expression of XIST in Huh7 cells after transfection with si-XIST or si-Scramble. ** vs Blank, p < 0.01. (B) Relative expression of XIST in HCCLM3 cells after transfection with pcDNA-XIST or pcDNA-Scramble. ## vs Blank, p < 0.01. (TIFF 164 kb)
12885_2017_3216_MOESM3_ESM.tif
Additional file 4: Figure S2. (A) The efficiency of miR-181a inhibitor transfected in HCCLM3 cells was evaluated by qRT-PCR. ** vs inhibitor NC, p < 0.01. (B) The efficiency of miR-181a mimics transfected in Huh7 cells was evaluated by qRT-PCR. ## vs mimics, p < 0.01. (TIFF 118 kb)
12885_2017_3216_MOESM4_ESM.tif
Literatur
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