Background
Methods
Patient population and tissue specimens
AQP gene expression analysis by data mining of independent NSCLC microarray studies
Quantitative real-time PCR
Tissue microarray construction
Immunohistochemistry and statistical analyses
Western blotting
Results
Comprehensive AQP expression analyses in NSCLC reveal a predominant expression of AQP1, -3, and -4 in adenocarcinomas
AQP isoform | Microarray Data | qRT-PCR Data | Median Ct value in tissues | Pearson correlation of AQPs | |||||
---|---|---|---|---|---|---|---|---|---|
Tumor vs Normal Fold change | AC vs SCC Fold change | Tumor vs Normal Fold change | AC vs SCC Fold change | AQP1 | AQP3 | AQP4 | AQP5 | ||
AQP1 |
0.18*
|
7.5*
|
0.22*
|
2.36*
| 27.8 | X | x | x | x |
AQP3 | 1.00 |
4.8*
| 0.93 |
5.81*
| 26.9 | 0.46 | x | x | x |
AQP4 |
0.16*
|
2.8*
|
0.11*
|
5.96*
| 28.7 |
0.82
| 0.42 | x | x |
AQP5 | 0.94 |
6.9*
|
0.50*
| 1.88 | 31.8 | 0.31 | 0.40 | 0.24 | x |
AQP9 |
0.26*
| 0.91 | 1.05 | 0.64 | 31.0 | 0.39 | 0.34 | 0.30 | 0.10 |
AQP isoform | Tumor vs Normal | AC vs SCC | AC MT vs MN | SCC MT vs MN | Survival in AC: Good vs Bad | |||||
---|---|---|---|---|---|---|---|---|---|---|
p-value | Fold change | p-value | Fold change | p-value | Fold change | p-value | Fold change | p-value | Fold change | |
AQP1 |
0.0000
|
0.22
|
0.0228
|
2.36
|
0.0000
|
0.26
|
0.0000
|
0.10
|
0.0340
|
2.86
|
AQP3 | 0.7089 | 0.93 |
0.0005
|
5.81
| 0.0205 | 1.76 | 0.0676 | 0.38 | 0.7165 | 0.81 |
AQP4 |
0.0000
|
0.11
|
0.0144
|
5.96
|
0.0000
|
0.12
|
0.0002
|
0.02
|
0.0344
|
4.42
|
AQP5 |
0.0253
|
0.50
| 0.3255 | 1.88 | 0.1640 | 0.55 | 0.1956 | 0.39 | 0.1937 | 0.25 |
AQP9 | 0.7384 | 1.05 | 0.1310 | 0.64 | 0.6103 | 0.91 | 0.7887 | 1.12 | 0.7480 | 1.15 |
AQP4-protein is more abundant in well-differentiated adenocarcinomas
Analyses of AQP4-coexpressed genes reveal an association to basic lung functions and respiratory disorders
Relevant Functions and Diseases | No. of genes | Examples (Gene Symbol) |
---|---|---|
Cancer | 65 | CALCRL, CDC2, CDH5, DMBT1, EZH2, FABP4, FOLR1, ROS1, THBS2 |
Respiratory Disease | 25 | ANGPT1, BUB1B, CAV1, EDNRB, PLK1, RRM1, TGFBR2, TOP2A, VWF |
Cell Cycle | 17 | BUB1B, CDC45L, CDKN3, CENPF, CITED2, MYBL2, PTTG1, RPS6KA2 |
Lipid Metabolism | 15 | CAT, CAV1, CYP27A1, FABP4, LPL, SEPP1, SFTPA1, SFTPB, SFTPC, SFTPD |
Molecular Transport | 17 | ABCA3, AGTR2, AQP1, CFD, CYP27A1 NME1 |
Small Molecule Biochemistry | 30 | A2M, AOC3, C7, CAT, DIO2, GPX3, LPL, NPR1, PFN2, SEPP1, TEK, TK1 |
Cellular Movement | 35 | CAT, CTSE, DLC1, ESAM, ICAM2, PLA2G1B, S1PR1, SFTPC, SFTPD, VIPR1 |
Cell-to-Cell Signalling | 30 | C4BPA, COL4A3, DPYSL2, PTPRB, SLC6A4, SULF1, THY1, TROAP |