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28.02.2024 | Original Article

Methylseleninic acid inhibits human glioma growth in vitro and in vivo by triggering ROS-dependent oxidative damage and apoptosis

verfasst von: Wang Chen, Pida Hao, Qile Song, Xiaotong Feng, Xuan Zhao, Jincheng Wu, Zixiang Gong, Jinli Zhang, Xiaoyan Fu, Xianjun Wang

Erschienen in: Metabolic Brain Disease | Ausgabe 4/2024

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Abstract

Selenium-containing agents showed novel anticancer activity by triggering pro-oxidative mechanism. Studies confirmed that methylseleninic acid (MeSe) displayed broad-spectrum anti-tumor activity against kinds of human cancers. However, the anticancer effects and mechanism of MeSe against human glioma growth have not been explored yet. Herein, the present study showed that MeSeA dose-dependently inhibited U251 and U87 human glioma cells growth in vitro. Flow cytometry analysis indicated that MeSe induced significant U251 cells apoptosis with a dose-dependent manner, followed by the activation of caspase-7, caspase-9 and caspase-3. Immunofluorescence staining revealed that MeSe time-dependently caused reactive oxide species (ROS) accumulation and subsequently resulted in oxidative damage, as convinced by the increased phosphorylation level of Ser428-ATR, Ser1981-ATM, Ser15-p53 and Ser139-histone. ROS inhibition by glutathione (GSH) effectively attenuated MeSe-induced ROS generation, oxidative damage, caspase-3 activation and cytotoxicity, indicating that ROS was an upstream factor involved in MeSe-mediated anticancer mechanism in glioma. Importantly, MeSe administration in nude mice significantly inhibited glioma growth in vivo by inducing apoptosis through triggering oxidative damage. Taken together, our findings validated the possibility that MeSe as a selenium-containing can act as potential tumor chemotherapy agent for therapy of human glioma.

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Titel: Methylseleninic acid inhibits human glioma growth in vitro and in vivo by triggering ROS-dependent oxidative damage and apoptosis
verfasst von: Wang Chen
Pida Hao
Qile Song
Xiaotong Feng
Xuan Zhao
Jincheng Wu
Zixiang Gong
Jinli Zhang
Xiaoyan Fu
Xianjun Wang
Publikationsdatum: 28.02.2024
Verlag: Springer US
Erschienen in: Metabolic Brain Disease / Ausgabe 4/2024
Print ISSN: 0885-7490
Elektronische ISSN: 1573-7365
DOI: https://doi.org/10.1007/s11011-024-01344-5

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