nach oben

Metabolic Brain Disease

Erschienen in:

12.04.2021 | Original Article

MiRNA-34c-5p protects against cerebral ischemia/reperfusion injury: involvement of anti-apoptotic and anti-inflammatory activities

verfasst von: Yaoran Tu, Yong Hu

Erschienen in: Metabolic Brain Disease | Ausgabe 6/2021

Einloggen, um Zugang zu erhalten

Abstract

MicroRNAs (miRNAs) are known as important regulators of gene expression and play important roles in diverse biological activities. However, the involvement of miRNAs in cerebral ischemia remains elusive. In the present study, using the middle cerebral artery occlusion (MCAO) model and oxygen-glucose deprivation/reperfusion (OGD/RP)-induced cell injury model, we found that the expression levels of miR-34c-5p were significantly reduced in MCAO rats and OGD/RP cells. Overexpression of miR-34c-5p could improve the increased brain infarction, brain water content and neurological scores in MCAO rats, as well as the abnormal expression of inflammatory cytokines (TNF-α, IL-6, COX-2, iNOS, IL-10) in OGD/RP cells. Moreover, overexpression of miR-34c-5p was found to inhibit the activity of nuclear factor-kappa B (NF-κB) by regulating the expression of nuclear receptor coactivator 1 (NCOA1), and increase the apoptotic rate of cortical neurons by inhibiting the expression of Caspase-3 and Bax and upregulating the expression of Bcl-2. Taken together, our findings demonstrated that miR-34c-5p plays an important role in cerebral ischemia/reperfusion injury, which may be mediated through inflammatory and apoptotic signaling pathways.

Nur mit Berechtigung zugänglich

Bracard S, Ducrocq X, Mas JL, Soudant M, Oppenheim C, Moulin T, Guillemin F, THRACE investigators (2016) Mechanical thrombectomy after intravenous alteplase versus alteplase alone after stroke (THRACE): a randomised controlled trial. Lancet Neurol 15(11):1138–1147PubMedCrossRef

Braicu OL, Budisan L, Buiga R, Jurj A, Achimas-Cadariu P, Pop L, Braicu C, Irimie A, Berindan-Neagoe I (2017) miRNA expression profiling in formalin-fixed paraffin-embedded endometriosis and ovarian cancer samples. Oncotarget Ther 10:4225–4238CrossRef

De SÉ, Walter LT et al (2013) Developmental and functional expression of miRNA-stability related genes in the nervous system. PLoS One 8(5):e56908CrossRef

Deng XH, Zhong Y, Gu LZ, Shen W, Guo J (2013) MiR-21 involve in ERK-mediated upregulation of MMP9 in the rat hippocampus following cerebral ischemia. Brain Res Bull 94:56–62PubMedCrossRef

Deng Y, Ma G, Dong Q, Sun X, Liu L, Miao Z, Gao F (2019) Overexpression of miR4 alleviates apoptosis from cerebral ischemia reperfusion injury by targeting FIP200. J Cell Biochem 120(10):17151–17158PubMedCrossRef

Fan YY, Hu WW et al (2017) Postconditioning-induced neuroprotection, mechanisms and applications in cerebral ischemia. Neurochem Int 107:S0197018616303680CrossRef

Fang H, Li HF et al. (2021) HDAC3 Downregulation improves cerebral ischemic injury via regulation of the SDC1-dependent JAK1/STAT3 signaling pathway through miR-19a Upregulation. Molecular Neurobiology

Gao G, Tian Z et al. (2018) miRNA-133b targets FGFR1 and presents multiple tumor suppressor activities in osteosarcoma. Cancer cell international 18(1)

Hu GQ, Du X et al (2017) Inhibition of cerebral ischemia/reperfusion injury-induced apoptosis: nicotiflorin and JAK2/STAT3 pathway. Neural Regen Res 12(1):96–102PubMedPubMedCentralCrossRef

Hu ZG, Zhao PX et al (2020) Hsa_circ_0011290 regulates proliferation, apoptosis and glycolytic phenotype in papillary thyroid cancer via miR-1252/FSTL1 signal pathway. Arch Biochem Biophys 685:108353PubMedCrossRef

Khodanovich MY, Akulov AE, Anan’ina TV, Kudabaeva MS, Pishchelko AO, Krutenkova EP, Nemirovich-Danchenko NM, Svetlik MV, Tumentceva YA, van den Haute C, Gijsbers R, Daniëls V, Thiry I, Pershina AG, Shadrina MM, Naumova AV (2020) Tissue-specific ferritin- and GFP-based genetic vectors visualize neurons by MRI in the intact and post-ischemic rat brain. Int J Mol Sci 21(23)

Kim JO, Bae J, Kim J, Oh SH, An HJ, Han IB, Oh D, Kim OJ, Kim NK (2018) Association of MicroRNA biogenesis genes polymorphisms with ischemic stroke susceptibility and post-stroke mortality. J Stroke 20(1):110–121PubMedPubMedCentralCrossRef

Lerner M, Lundgren J, Akhoondi S, Jahn A, Ng HF, Moqadam FA, Oude Vrielink JAF, Agami R, den Boer ML, Grandér D, Sangfelt O (2011) MiRNA-27a controls FBW7/hCDC4-dependent cyclin E degradation and cell cycle progression. Cell Cycle 10(13):2172–2183PubMedCrossRef

Li JH, Wang YH et al (2010) Developmental changes in localization of NMDA receptor subunits in primary cultures of cortical neurons. Eur J Neurosci 10(5):1704–1715CrossRef

Liang Y, Xu J, Wang Y, Tang JY, Yang SL, Xiang HG, Wu SX, Li XJ (2018) Inhibition of MiRNA-125b decreases cerebral ischemia/reperfusion injury by targeting CK2α/NADPH oxidase signaling. Cell Physiol Biochem Int J Exp Cell Physiol Biochem Pharmacol 45(5):1818–1826CrossRef

Liu Y, Wang H, Yang M, Liu N, Zhao Y, Qi X, Niu Y, Sun T, Li Y, Yu J (2018) Cistanche deserticola polysaccharides protects PC12 cells against OGD/RP-induced injury. Biomed Pharmacother 99:671–680PubMedCrossRef

Malhas A, Saunders NJ, Vaux DJ (2010) The nuclear envelope can control gene expression and cell cycle progression via miRNA regulation. Cell Cycle 9(3):531–539PubMedCrossRef

Men M, Zhang L, Li T, Mi B, Wang T, Fan Y, Chen Y, Shen G, Liang L, Ma A (2015) Prognostic value of the percentage of neutrophils on admission in patients with ST-elevated myocardial infarction undergoing primary percutaneous coronary intervention. Arch Med Res 46(4):274–279PubMedCrossRef

Meng QT, Chen R, Chen C, Su K, Li W, Tang LH, Liu HM, Xue R, Sun Q, Leng Y, Hou JB, Wu Y, Xia ZY (2017) Transcription factors Nrf2 and NF-κB contribute to inflammation and apoptosis induced by intestinal ischemia-reperfusion in mice. Int J Mol Med 40(6):1731–1740PubMedPubMedCentral

Moltzahn F, Olshen AB, Baehner L, Peek A, Fong L, Stöppler H, Simko J, Hilton JF, Carroll P, Blelloch R (2011) Microfluidic-based multiplex qRT-PCR identifies diagnostic and prognostic microRNA signatures in the sera of prostate Cancer patients. Cancer Res 71(2):550–560PubMedCrossRef

Ning Z, Xiang Y et al. (2017) Schisandrin B prevents hind limb from ischemia-reperfusion-induced oxidative stress and inflammation via MAPK/NF-κB pathways in rats. 2017(5): 1-8

Park J, Chung T et al (2017) Ascofuranone inhibits lipopolysaccharide–induced inflammatory response via NF-kappaB and AP-1, p-ERK, TNF-α, IL-6 and IL-1β in RAW 264.7 macrophages. PloS One 12(2):e0171322PubMedPubMedCentralCrossRef

Pride GL, Fraser JF, Gupta R, Alberts MJ, Rutledge JN, Fowler R, Ansari SA, Abruzzo T, Albani B, Arthur A, Baxter B, Bulsara KR, Chen M, Delgado Almandoz JE, Gandhi CD, Heck D, Hetts SW, Hirsch JA, Hussain MS, Klucznik R, Lee SK, Mack WJ, Leslie-Mazwi T, McTaggart R, Meyers PM, Mocco J, Prestigiacomo C, Patsalides A, Rasmussen P, Starke RM, Sunenshine P, Frei D, Jayaraman MV, Standards and Guidelines Committee of the Society of NeuroInterventional Surgery (SNIS) (2017) Prehospital care delivery and triage of stroke with emergent large vessel occlusion (ELVO): report of the standards and guidelines Committee of the Society of Neurointerventional surgery. J NeuroIntervention Surg 9(8):802–812CrossRef

Qin L, Wu YL, Toneff MJ, Li D, Liao L, Gao X, Bane FT, Tien JCY, Xu Y, Feng Z, Yang Z, Xu Y, Theissen SM, Li Y, Young L, Xu J (2014) NCOA1 directly targets M-CSF1 expression to promote breast Cancer metastasis. Cancer Res 74(13):3477–3488PubMedPubMedCentralCrossRef

Rosca AM, Matei C et al (2012) Cardiomyocyte apoptosis in ischaemia-reperfusion due to the exogenous oxidants at the time of reperfusion. Cell Biol Int 36(12):1207–1215PubMedCrossRef

Salakou S, Kardamakis D et al (2007) Increased Bax/Bcl-2 ratio up-regulates caspase-3 and increases apoptosis in the thymus of patients with myasthenia gravis. Vivo 21(1):123

Saver JL, Levine SR (2010) Alteplase for ischaemic stroke--much sooner is much better. Lancet 375(9727):1667–1668PubMedCrossRef

Sayre KR, Dodd RY et al (2010) False–positive human immunodeficiency virus type 1 western blot tests in noninfected blood donors. Transfusion 36(1):45–52CrossRef

Shi J, Dai W et al. (2017) Therapeutic hypothermia reduces the inflammatory response following ischemia/reperfusion injury in rat hearts. Therapeutic Hypothermia & Temperature Management

Wang XY, Chen SS et al. (2018) miRNA-3473b contributes to neuroinflammation following cerebral ischemia. Cell Death Dis 9

Wu H, Huang M, Lu M, Zhu W, Shu Y, Cao P, Liu P (2013) Regulation of microtubule-associated protein tau (MAPT) by miR-34c-5p determines the chemosensitivity of gastric cancer to paclitaxel. Cancer Chem Pharmacol 71(5):1159–1171CrossRef

Xia M, Ding Q et al (2017) Remote limb ischemic preconditioning protects rats against cerebral ischemia via HIF-1α/AMPK/HSP70 pathway. Cell Mol Neurobiol 37(6):1–10CrossRef

Xiao YM, Zhang YX et al (2019) Inhibition of MicroRNA-9-5p protects against cardiac remodeling following myocardial infarction in mice. Hum Gene Ther 30(3):286–301PubMedCrossRef

Yu H, Wu M, Zhao P, Huang Y, Wang W, Yin W (2015) Neuroprotective effects of viral overexpression of microRNA–22 in rat and cell models of cerebral ischemia–reperfusion injury. J Cell Biochem 116(2):233–241PubMedCrossRef

Yun HC, Na BH et al (2016) Anti-inflammatory function of 4-tert-butylphenyl salicylate through down-regulation of the NF-kappa B pathway. Arch Pharm Res 39(3):1–8

Zhang G-Y et al (2014b) Expression of Bcl-2 and NF-κB in brain tissue after acute renal ischemia-reperfusion in rats. Asian Pac J Trop Med 7(5):386–389PubMedCrossRef

Zhang MJ, Wu XW et al (2017) The cystathionine beta-synthase/hydrogen sulfide pathway contributes to microglia-mediated neuroinflammation following cerebral ischemia. Brain Behav Immun 66:332–346PubMedCrossRef

Zhang R, Wugeti N, Sun J, Yan H, Guo Y, Zhang L, Ma M, Guo X, Jiao C, Xu W, Li T, Liu H, Ma Y (2014a) Effects of vagus nerve stimulation via cholinergic anti-inflammatory pathway activation on myocardial ischemia/reperfusion injury in canine. Int J Clinic Exp Med 7(9):2615–2623

Zhao Q, Cui Z, Zheng Y, Li Q, Xu C, Sheng X, Tao M, Xu HX (2017) Fenofibrate protects against acute myocardial I/R injury in rat by suppressing mitochondrial apoptosis as decreasing cleaved caspase-9 activation. Cancer Biomark 19(4):455–463PubMedCrossRef

Zhou Y, Chen Q, Lew KS, Richards AM, Wang P (2016) Discovery of potential therapeutic miRNA targets in cardiac ischemia-reperfusion injury. J Cardiovasc Pharmacol Ther 21(3):296–309PubMedCrossRef

Titel: MiRNA-34c-5p protects against cerebral ischemia/reperfusion injury: involvement of anti-apoptotic and anti-inflammatory activities
verfasst von: Yaoran Tu
Yong Hu
Publikationsdatum: 12.04.2021
Verlag: Springer US
Erschienen in: Metabolic Brain Disease / Ausgabe 6/2021
Print ISSN: 0885-7490
Elektronische ISSN: 1573-7365
DOI: https://doi.org/10.1007/s11011-021-00724-5

Leitlinien kompakt für die Neurologie

Mit medbee Pocketcards sicher entscheiden.

^{Seit 2022 gehört die medbee GmbH zum Springer Medizin Verlag}

Kostenlos registrieren

Neu im Fachgebiet Neurologie

Hirnblutung unter DOAK und VKA ähnlich bedrohlich

17.05.2024 Direkte orale Antikoagulanzien Nachrichten

Kommt es zu einer nichttraumatischen Hirnblutung, spielt es keine große Rolle, ob die Betroffenen zuvor direkt wirksame orale Antikoagulanzien oder Marcumar bekommen haben: Die Prognose ist ähnlich schlecht.

Thrombektomie auch bei großen Infarkten von Vorteil

16.05.2024 Ischämischer Schlaganfall Nachrichten

Auch ein sehr ausgedehnter ischämischer Schlaganfall scheint an sich kein Grund zu sein, von einer mechanischen Thrombektomie abzusehen. Dafür spricht die LASTE-Studie, an der Patienten und Patientinnen mit einem ASPECTS von maximal 5 beteiligt waren.

Schwindelursache: Massagepistole lässt Otholiten tanzen

14.05.2024 Benigner Lagerungsschwindel Nachrichten

Wenn jüngere Menschen über ständig rezidivierenden Lagerungsschwindel klagen, könnte eine Massagepistole der Auslöser sein. In JAMA Otolaryngology warnt ein Team vor der Anwendung hochpotenter Geräte im Bereich des Nackens.

Schützt Olivenöl vor dem Tod durch Demenz?

10.05.2024 Morbus Alzheimer Nachrichten

Konsumieren Menschen täglich 7 Gramm Olivenöl, ist ihr Risiko, an einer Demenz zu sterben, um mehr als ein Viertel reduziert – und dies weitgehend unabhängig von ihrer sonstigen Ernährung. Dafür sprechen Auswertungen zweier großer US-Studien.

Update Neurologie

Bestellen Sie unseren Fach-Newsletter und bleiben Sie gut informiert.

Newsletter bestellen

Die Highlights vom Kongress des American College of Cardiology 2024

Springer Medizin

Abstract

Bitte loggen Sie sich ein, um Zugang zu diesem Inhalt zu erhalten

Weitere Artikel der Ausgabe 6/2021

DNACJ12 deficiency in patients with unexplained hyperphenylalaninemia: two new patients and a novel variant

Neurovascular dysfunctions in hypertensive disorders of pregnancy

Thioacetamide-induced acute hepatic encephalopathy: central vs peripheral effect of Allicin

Pre-micro RNA polymorphism detection in small versus large vessel disease in stroke Egyptian patients

The functions of repressor element 1-silencing transcription factor in models of epileptogenesis and post-ischemia