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01.11.2014 | Original Article

Molecular Mechanism of Acute Radiation Enteritis Revealed Using Proteomics and Biological Signaling Network Analysis in Rats

verfasst von: Shunxin Song, Dianke Chen, Tenghui Ma, Yanxin Luo, Zuli Yang, Daohai Wang, Xinjuan Fan, Qiyuan Qin, Beibei Ni, Xuefeng Guo, Zhenyu Xian, Ping Lan, Xinping Cao, Mingtao Li, Jianping Wang, Lei Wang

Erschienen in: Digestive Diseases and Sciences | Ausgabe 11/2014

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Abstract

Background and Aims

Radiation enteritis (RE) has emerged as a significant complication that can progress to severe gastrointestinal disease and the mechanisms underlying its genesis remain poorly understood. The aim of this study was to identify temporal changes in protein expression potentially associated with acute inflammation and to elucidate the mechanism underlying radiation enteritis genesis.

Methods

Male Sprague–Dawley rats were irradiated in the abdomen with a single dose of 10 Gy to establish an in vivo model of acute radiation enteritis. Two-dimensional fluorescence difference gel electrophoresis, matrix-assisted laser desorption/ionization time-of-flight spectrometer (MALDI-TOF) tandem mass spectrometry, and peptide mass fingerprinting were used to determine differentially expressed proteins between normal and inflamed intestinal mucosa. Additionally, differentially expressed proteins were evaluated by KO Based Annotation System to find the biological functions associated with acute radiation enteritis.

Results

Intensity changes of 86 spots were detected with statistical significance (ratio ≥ 1.5 or ≤ 1.5, P < 0.05). Sixty one of the 86 spots were identified by MALDI-TOF/TOF tandem mass spectrometry. These radiation-induced proteins with biological functions showed that the FAS pathway and glycolysis signaling pathways were significantly altered using the KOBAS tool.

Conclusions

Our results reveal an underlying mechanism of radiation-induced acute enteritis, which may help clarify the pathogenesis of RE and point to potential targets for therapeutic interventions.

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Titel: Molecular Mechanism of Acute Radiation Enteritis Revealed Using Proteomics and Biological Signaling Network Analysis in Rats
verfasst von: Shunxin Song
Dianke Chen
Tenghui Ma
Yanxin Luo
Zuli Yang
Daohai Wang
Xinjuan Fan
Qiyuan Qin
Beibei Ni
Xuefeng Guo
Zhenyu Xian
Ping Lan
Xinping Cao
Mingtao Li
Jianping Wang
Lei Wang
Publikationsdatum: 01.11.2014
Verlag: Springer US
Erschienen in: Digestive Diseases and Sciences / Ausgabe 11/2014
Print ISSN: 0163-2116
Elektronische ISSN: 1573-2568
DOI: https://doi.org/10.1007/s10620-014-3224-1

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Background and Aims

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Results

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