nach oben

The Cerebellum

Erschienen in:

01.09.2009

Phospholipase Cβ4 Expression Identifies a Novel Subset of Unipolar Brush Cells in the Adult Mouse Cerebellum

verfasst von: Seung-Hyuk Chung, Hassan Marzban, Masahiko Watanabe, Richard Hawkes

Erschienen in: The Cerebellum | Ausgabe 3/2009

Einloggen, um Zugang zu erhalten

Abstract

Unipolar brush cells (UBCs) are glutamatergic cerebellar interneurons of the granular layer. Previous studies have shown that there are two distinct subsets of UBCs present in the mice cerebellar cortex: calcium-binding protein calretinin (CR) positive and metabotropic glutamate receptor (mGluR)1α positive. In this study, we identify phospholipase C (PLC) β4 as an antigenic marker of a novel subset of UBCs. Double immunolabeling reveals that none of the CR+ subset expresses PLCβ4. In contrast, most members of the mGluR1α subset also express PLCβ4. In addition, 65% of the PLCβ4+ subset does not express mGluR1α. Thus, there are three distinct UBC subsets in the mouse cerebellum: CR+/PLCβ4−/mGluR1α−, PLCβ4+/mGluR1α−/CR−, and mGluR1α+/PLCβ4+/CR−. Each has a different topographical distribution, both between lobules and mediolaterally within the vermis. The development of PLCβ4 expression in UBCs is exclusively postnatal—first seen only at P12 and mature at about 3 weeks. A distinct subset of PLCβ4+ UBCs is also present in primary cerebellar cultures.

Altman J, Bayer SA (1977) Time of origin and distribution of a new cell type in the rat cerebellar cortex. Exp Brain Res 29:265–274PubMedCrossRef

Mugnaini E, Floris A (1994) The unipolar brush cell: a neglected neuron of the mammalian cerebellar cortex. J Comp Neurol 339:174–180PubMedCrossRef

Dino MR, Nunzi MG, Anelli R, Mugnaini E (2000) Unipolar brush cells of the vestibulocerebellum: afferents and targets. Prog Brain Res 124:123–137PubMedCrossRef

Nunzi MG, Mugnaini E (2000) Unipolar brush cell axons form a large system of intrinsic mossy fibers in the postnatal vestibulocerebellum. J Comp Neurol 422:55–65PubMedCrossRef

Nunzi MG, Birnstiel S, Bhattacharyya BJ, Slater NT, Mugnaini E (2001) Unipolar brush cells form a glutamatergic projection system within the mouse cerebellar cortex. J Comp Neurol 434:329–341PubMedCrossRef

Floris A, Dino M, Jacobowitz DM, Mugnaini E (1994) The unipolar brush cells of the rat cerebellar cortex and cochlear nucleus are calretinin-positive: a study by light and electron microscopic immunocytochemistry. Anat Embryol Berl 189:495–520PubMedCrossRef

Nunzi MG, Shigemoto R, Mugnaini E (2002) Differential expression of calretinin and metabotropic glutamate receptor mGluR1 alpha defines subsets of unipolar brush cells in mouse cerebellum. J Comp Neurol 451:189–199PubMedCrossRef

Sekerková G, Ilijic E, Mugnaini E (2004) Time of origin of unipolar brush cells in the rat cerebellum as observed by prenatal bromodeoxyuridine labeling. Neuroscience 127:845–858PubMedCrossRef

Nunzi MG, Russo M, Mugnaini E (2003) Vesicular glutamate transporters VGLUT1 and VGLUT2 define two subsets of unipolar brush cells in organotypic cultures of mouse vestibulocerebellum. Neuroscience 122:359–371PubMedCrossRef

10.

Nakamura M, Sato K, Fukaya M, Araishi K, Aiba A, Kano M et al (2004) Signaling complex formation of phospholipase Cβ4 with metabotropic glutamate receptor type 1alpha and 1,4,5-triphosphate receptor at the perisynapse and endoplasmic reticulum in the mouse brain. Eur J Neurosci 20:2929–2944PubMedCrossRef

11.

Zeilhofer HU, Studler B, Arabadzisz D, Schweizer C, Ahmadi S, Layh B et al (2005) Glycinergic neurons expressing enhanced green fluorescent protein in bacterial artificial chromosome transgenic mice. J Comp Neurol 482:123–141PubMedCrossRef

12.

Feng G, Mellor RH, Bernstein M, Keller-Peck C, Nguyen QT, Wallace M et al (2000) Imaging neuronal subsets in transgenic mice expressing multiple spectral variants of GFP. Neuron 28:41–51PubMedCrossRef

13.

Brochu G, Maler L, Hawkes R (1990) Zebrin II: a polypeptide antigen expressed selectively by Purkinje cells reveals compartments in rat and fish cerebellum. J Comp Neurol 291:538–552PubMedCrossRef

14.

Abo T, Balch CM (1981) A differentiation antigen of human NK and K cells identified by a monoclonal antibody. J Immunol 127:1024–1029PubMed

15.

Eisenman LM, Hawkes R (1993) Antigenic compartmentation in the mouse cerebellar cortex: zebrin and HNK-1 reveal a complex, overlapping molecular topography. J Comp Neurol 335:586–605PubMedCrossRef

16.

Marzban H, Sillitoe RV, Hoy M, Chung SH, Rafuse VR, Hawkes R (2004) Abnormal HNK-1 expression in the cerebellum of an N-CAM null mouse. J Neurocytol 33:117–130PubMedCrossRef

17.

Schwaller B, Buchwald P, Blümcke I, Celio MR, Hunziker W (1994) Characterization of a polyclonal antiserum against the purified recombinant calcium-binding protein calretinin. Cell Calcium 14:639–648CrossRef

18.

Tanaka J, Nakagawa S, Kushiya E, Yamasaki M, Fukaya M, Iwanaga T et al (2000) Gq protein α subunits Gαq and Gα11 are localized at postsynaptic extra-junctional membrane of cerebellar Purkinje cells and hippocampal pyramidal cells. Eur J Neurosci 12:781–792PubMedCrossRef

19.

Sarna JR, Marzban H, Watanabe M, Hawkes R (2006) Complementary stripes of phospholipase Cβ3 and Cβ4 expression by Purkinje cell subsets in the mouse cerebellum. J Comp Neurol 496:303–313PubMedCrossRef

20.

Jiang H, Lyubarsky A, Dodd R, Vardi N, Pugh E, Baylor D et al (1996) Phospholipase C β4 is involved in modulating the visual response in mice. Proc Natl Acad Sci U S A 93:14598–14601PubMedCrossRef

21.

Sillitoe RV, Benson MA, Blake DJ, Hawkes R (2003) Abnormal dysbindin expression in cerebellar mossy fiber synapses in the mdx mouse model of Duchenne muscular dystrophy. J Neurosci 23:6576–6585PubMed

22.

Furuya S, Makino A, Hirabayashi Y (1998) An improved method for culturing cerebellar Purkinje cells with differentiated dendrites under a mixed monolayer setting. Brain Res Prot 3:192–198CrossRef

23.

Tabata T, Sawada S, Araki K, Bono Y, Furuya S, Kano M (2000) A reliable method for culture of dissociated mouse cerebellar cells enriched for Purkinje neurons. J Neurosci Meth 104:45–53CrossRef

24.

Marzban H, Khanzada U, Shabir S, Hawkes R, Langnaese K, Smalla K et al (2003) Expression of the immunoglobulin superfamily neuroplastin adhesion molecules in adult and developing mouse cerebellum and their location to parasagittal stripes. J Comp Neurol 462:286–301PubMedCrossRef

25.

Ozol K, Hayden JM, Oberdick J, Hawkes R (1999) Transverse zones in the vermis of the mouse cerebellum. J Comp Neurol 412:95–111PubMedCrossRef

26.

Armstrong C, Krueger-Naug AM, Currie WC, Hawkes R (2000) Constitutive expression of the 25 kDa heat shock protein Hsp25 reveals novel parasagittal bands of Purkinje cells in the adult mouse cerebellar cortex. J Comp Neurol 416:383–397PubMedCrossRef

27.

Sillitoe RV, Hawkes R (2002) Whole-mount immunohistochemistry: a high-throughput screen for patterning defects in the mouse cerebellum. J Histochem Cytochem 50:235–244PubMed

28.

Hawkes R, Brochu G, Doré L, Gravel C, Leclerc N (1992) Zebrins: molecular markers of compartmentation in the cerebellum. In: Llinás R, Sotelo C (eds) The cerebellum revisited. Springer, New York, NY, pp 22–55

29.

Hawkes R (1997) An anatomical model of cerebellar modules. Prog Brain Res 114:39–52PubMedCrossRef

30.

Herrup K, Kuemerle B (1997) The compartmentalization of the cerebellum. Ann Rev Neurosci 20:61–90PubMedCrossRef

31.

Oberdick J, Baader SL, Schilling K (1998) From zebra stripes to postal zones: deciphering patterns of gene expression in the cerebellum. Trends Neurosci 21:383–390PubMedCrossRef

32.

Ji Z, Hawkes R (1994) Topography of Purkinje cell compartments and mossy fiber terminal fields in lobules II and III of the rat cerebellar cortex: spinocerebellar and cuneocerebellar projections. Neuroscience 61:935–954PubMedCrossRef

33.

Akintunde A, Eisenman LM (1994) External cuneocerebellar projections and Purkinje cell zebrin II bands: a direct comparison of parasagittal banding in the mouse cerebellum. J Chem Neuroanat 7:75–86PubMedCrossRef

34.

Voogd J, Gerrits NM, Ruigrok TJ (1996) Organization of the vestibulocerebellum. Ann N Y Acad Sci 781:553–579PubMedCrossRef

35.

Yan XX, Yen LS, Garey LJ (1993) Parasagittal patches in the granular layer of the developing and adult rat cerebellum as demonstrated by NADPH-diaphorase histochemistry. NeuroReport 4:1227–1230PubMedCrossRef

36.

Hawkes R, Turner RW (1994) Compartmentation of NADPH-diaphorase activity in the mouse cerebellar cortex. J Comp Neurol 346:499–516PubMedCrossRef

37.

Schilling K, Schmidt HH, Baader SL (1994) Nitric oxide synthase expression reveals compartments of cerebellar granule cells and suggests a role for mossy fibers in their development. Neuroscience 59:893–903PubMedCrossRef

38.

Ozol KO, Hawkes R (1997) The compartmentation of the granular layer of the cerebellum. Histol Histopathol 12:171–184PubMed

39.

Ahn AH, Dziennis S, Hawkes R, Herrup K (1994) The cloning of zebrin II reveals its identity with aldolase C. Development 120:2081–2090PubMed

40.

Hawkes R, Herrup K (1996) Aldolase C/zebrin II and the regionalization of the cerebellum. J Mol Neurobiol 6:147–158CrossRef

41.

Abbott LC, Jacobowitz DM (1995) Development of calretinin-immunoreactive unipolar brush-like cells and an afferent pathway to the embryonic and early postnatal mouse cerebellum. Anat Embryol Berl 191:541–559PubMedCrossRef

42.

Marzban H, Chung S, Watanabe M, Hawkes R (2007) Phospholipase Cβ4 expression reveals the continuity of cerebellar topography through development. J Comp Neurol 502:857–871PubMedCrossRef

43.

Anelli R, Mugnaini E (2001) Enrichment of unipolar brush cell-like neurons in primary rat cerebellar cultures. Anat Embryol (Berl) 203:283–292CrossRef

Titel: Phospholipase Cβ4 Expression Identifies a Novel Subset of Unipolar Brush Cells in the Adult Mouse Cerebellum
verfasst von: Seung-Hyuk Chung
Hassan Marzban
Masahiko Watanabe
Richard Hawkes
Publikationsdatum: 01.09.2009
Verlag: Springer-Verlag
Erschienen in: The Cerebellum / Ausgabe 3/2009
Print ISSN: 1473-4222
Elektronische ISSN: 1473-4230
DOI: https://doi.org/10.1007/s12311-009-0092-x

Leitlinien kompakt für die Neurologie

Mit medbee Pocketcards sicher entscheiden.

^{Seit 2022 gehört die medbee GmbH zum Springer Medizin Verlag}

Kostenlos registrieren

Neu im Fachgebiet Neurologie

Hirnblutung unter DOAK und VKA ähnlich bedrohlich

17.05.2024 Direkte orale Antikoagulanzien Nachrichten

Kommt es zu einer nichttraumatischen Hirnblutung, spielt es keine große Rolle, ob die Betroffenen zuvor direkt wirksame orale Antikoagulanzien oder Marcumar bekommen haben: Die Prognose ist ähnlich schlecht.

Thrombektomie auch bei großen Infarkten von Vorteil

16.05.2024 Ischämischer Schlaganfall Nachrichten

Auch ein sehr ausgedehnter ischämischer Schlaganfall scheint an sich kein Grund zu sein, von einer mechanischen Thrombektomie abzusehen. Dafür spricht die LASTE-Studie, an der Patienten und Patientinnen mit einem ASPECTS von maximal 5 beteiligt waren.

Schwindelursache: Massagepistole lässt Otholiten tanzen

14.05.2024 Benigner Lagerungsschwindel Nachrichten

Wenn jüngere Menschen über ständig rezidivierenden Lagerungsschwindel klagen, könnte eine Massagepistole der Auslöser sein. In JAMA Otolaryngology warnt ein Team vor der Anwendung hochpotenter Geräte im Bereich des Nackens.

Schützt Olivenöl vor dem Tod durch Demenz?

10.05.2024 Morbus Alzheimer Nachrichten

Konsumieren Menschen täglich 7 Gramm Olivenöl, ist ihr Risiko, an einer Demenz zu sterben, um mehr als ein Viertel reduziert – und dies weitgehend unabhängig von ihrer sonstigen Ernährung. Dafür sprechen Auswertungen zweier großer US-Studien.

Update Neurologie

Bestellen Sie unseren Fach-Newsletter und bleiben Sie gut informiert.

Newsletter bestellen

Die Highlights vom Kongress des American College of Cardiology 2024

Springer Medizin

Abstract

Bitte loggen Sie sich ein, um Zugang zu diesem Inhalt zu erhalten

Weitere Artikel der Ausgabe 3/2009

The Robotic Mouse: Understanding the Role of AF4, a Cofactor of Transcriptional Elongation and Chromatin Remodelling, in Purkinje Cell Function

Animal Models of Human Cerebellar Ataxias: a Cornerstone for the Therapies of the Twenty-First Century

Experience-Dependent Plasticity of Cerebellar Vermis in Basketball Players

The Role of Kv3-type Potassium Channels in Cerebellar Physiology and Behavior

Cerebellar Contributions to the Processing of Saccadic Errors