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Intensive Care Medicine

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01.03.2005 | Experimental

Subcutaneous gas tensions closely track ileal mucosal gas tensions in a model of endotoxaemia without anaerobism

verfasst von: Bala Venkatesh, Thomas J. Morgan, Jonathan Hall, Zolton Endre, Desley Willgoss

Erschienen in: Intensive Care Medicine | Ausgabe 3/2005

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Abstract

Objective

Few comparative data exist on the responses of the subcutaneous and splanchnic circulations to evolving endotoxic shock. We therefore compared continuous subcutaneous pO₂ (pO_2sc) and pCO₂ (pCO_2sc) with simultaneous continuous gut luminal pCO₂ (pCO_2gi) in an animal model of endotoxaemia and examined whether changes in gas tensions track tissue energy charge (EC).

Design

Prospective observational study.

Subjects

Fourteen anaesthetized rats, 7 controls and 7 experimental.

Interventions

Controls were injected with saline, the experimental group with 20 mg/kg Klebsiella endotoxin. pCO_2sc, pO_2sc, and pCO_2gi were measured continuously. Plasma lactate concentrations were measured at defined periods during the study. After 2 h ileal segments were snap frozen and assayed for tissue EC.

Measurements and results

Endotoxaemia resulted in a significant decrease in mean arterial blood pressure (132±9 to 71±20 mmHg) and pO_2sc (71±23 to 33±22 torr) and a significant increase in pCO_2gi (58±10 to 90±20 torr) and pCO_2sc (56±6 to 81±25 torr). During endotoxaemia pCO_2gi was directly correlated with pCO_2sc (R ²=0.5) and inversely correlated with pO_2sc (R ²=0.63). Plasma lactate concentrations were significantly elevated from baseline in the endotoxin limb. The mean EC was not significantly different in the two groups.

Conclusions

Both subcutaneous tissue gas tensions and intestinal luminal carbon dioxide tensions are rapidly responsive during evolving hypodynamic endotoxic shock. Alterations in tissue gas tensions were not associated with dysoxia.

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Titel: Subcutaneous gas tensions closely track ileal mucosal gas tensions in a model of endotoxaemia without anaerobism
verfasst von: Bala Venkatesh
Thomas J. Morgan
Jonathan Hall
Zolton Endre
Desley Willgoss
Publikationsdatum: 01.03.2005
Verlag: Springer-Verlag
Erschienen in: Intensive Care Medicine / Ausgabe 3/2005
Print ISSN: 0342-4642
Elektronische ISSN: 1432-1238
DOI: https://doi.org/10.1007/s00134-005-2558-8

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Subcutaneous gas tensions closely track ileal mucosal gas tensions in a model of endotoxaemia without anaerobism

Abstract

Objective

Design

Subjects

Interventions

Measurements and results

Conclusions

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Springer Medizin

Abstract

Objective

Design

Subjects

Interventions

Measurements and results

Conclusions

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