Skip to main content
main-content

01.12.2012 | Research | Ausgabe 1/2012 Open Access

Reproductive Biology and Endocrinology 1/2012

The effects of male age on sperm analysis by motile sperm organelle morphology examination (MSOME)

Zeitschrift:
Reproductive Biology and Endocrinology > Ausgabe 1/2012
Autoren:
Liliane FI Silva, Joao Batista A Oliveira, Claudia G Petersen, Ana L Mauri, Fabiana C Massaro, Mario Cavagna, Ricardo LR Baruffi, José G Franco Jr
Wichtige Hinweise

Electronic supplementary material

The online version of this article (doi:10.​1186/​1477-7827-10-19) contains supplementary material, which is available to authorized users.

Competing interests

The authors declare that they have no competing interests.

Authors' contributions

LFIS designed and coordinated the study. All authors were responsible for the data collection, analysis, and interpretation presented in the manuscript. LFIS, JBAO, CGP and JGF performed the statistical analyses and wrote the manuscript; JGF reviewed the manuscript. All authors read and approved the final manuscript.

Abstract

Background

This study aimed to investigate the influence of age on sperm quality, as analysed by motile sperm organelle morphology examination (MSOME).

Methods

Semen samples were collected from 975 men undergoing evaluation or treatment for infertility. Sperm cells were evaluated at 8400× magnification using an inverted microscope equipped with Nomarski (differential interference contrast) optics. Two forms of spermatozoa were considered: normal spermatozoa and spermatozoa with large nuclear vacuoles (LNV, defined as vacuoles occupying > 50% of the sperm nuclear area). At least 200 spermatozoa per sample were evaluated, and the percentages of normal and LNV spermatozoa were determined. The subjects were divided into three groups according to age: Group I, less than or equal to 35 years; Group II, 36-40 years; and Group III, greater than or equal to 41 years.

Results

There was no difference in the percentages of normal sperm between the two younger (I and II) groups (P > 0.05). The percentage of normal sperm in the older group (III) was significantly lower than that in the younger (I and II) groups (P < 0.05). There was no difference in the percentage of LNV spermatozoa between the younger (I and II) groups (P > 0.05). The percentage of LNV spermatozoa was significantly higher in the older group (III) than in the younger (I and II) groups (P < 0.05). Regression analysis demonstrated a significant decrease in the incidence of normal sperm with increasing age (P < 0.05; r = -0.10). However, there was a significant positive correlation between the percentage of spermatozoa with LNV and male age (P < 0.05, r = 0.10).

Conclusion

The results demonstrated a consistent decline in semen quality, as reflected by morphological evaluation by MSOME, with increased age. Considering the relationship between nuclear vacuoles and DNA damage, these age-related changes predict that increased paternal age should be associated with unsuccessful or abnormal pregnancy as a consequence of fertilisation with damaged spermatozoa. Given that sperm nuclear vacuoles can be evaluated more precisely at high magnification, these results support the routine use of MSOME for ICSI as a criterion for semen analysis.
Zusatzmaterial
Authors’ original file for figure 1
12958_2012_959_MOESM1_ESM.pdf
Authors’ original file for figure 2
12958_2012_959_MOESM2_ESM.jpeg
Authors’ original file for figure 3
12958_2012_959_MOESM3_ESM.jpeg
Authors’ original file for figure 4
12958_2012_959_MOESM4_ESM.jpeg
Authors’ original file for figure 5
12958_2012_959_MOESM5_ESM.jpeg
Literatur
Über diesen Artikel

Weitere Artikel der Ausgabe 1/2012

Reproductive Biology and Endocrinology 1/2012 Zur Ausgabe

Neu im Fachgebiet Gynäkologie und Geburtshilfe

Meistgelesene Bücher aus dem Fachgebiet

2014 | Buch

Perikonzeptionelle Frauenheilkunde

Fertilitätserhalt, Prävention und Management von Schwangerschaftsrisiken

Ein Praxisbuch für alle, die in der Beratung und Betreuung von Schwangeren und Paaren mit Kinderwunsch tätig sind. Die Herausgeber und Autoren haben alle wichtigen Informationen zusammengetragen, um Frauen sicher zu beraten, zu betreuen und kompetent Verantwortung zu übernehmen.

Herausgeber:
Christian Gnoth, Peter Mallmann

2014 | Buch

Praxisbuch Gynäkologische Onkologie

Das Nachschlagewerk für alle Ärzte in Klinik und Praxis, die Patientinnen mit bösartigen Tumoren des Genitales und der Mamma behandeln. Dieses Buch gibt in kompakter Weise einfach umsetzbare Handlungsanweisungen zum diagnostischen und therapeutischen Vorgehen und Hilfestellungen für die optimale Versorgung der Patientinnen.

Herausgeber:
Edgar Petru, Walter Jonat, Daniel Fink, Ossi R. Köchli

Mail Icon II Newsletter

Bestellen Sie unseren kostenlosen Newsletter Update Gynäkologie und bleiben Sie gut informiert – ganz bequem per eMail.

Bildnachweise