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The Cerebellum

Erschienen in:

01.09.2008

3′UTR-Dependent Localization of a Purkinje Cell Messenger RNA in Dendrites

verfasst von: Rui Zhang, Xulun Zhang, Feng Bian, Xin-an Pu, Karl Schilling, John Oberdick

Erschienen in: The Cerebellum | Ausgabe 3/2008

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Abstract

Pcp2(L7) is a Purkinje cell-specific GoLoco domain protein that modulates activation of Gαi/o proteins by G protein-coupled receptors. A likely downstream effector of this pathway is the P-type Ca²⁺ channel, and thereby, the intrinsic electrophysiology of Purkinje cells could be modulated by Pcp2(L7). It has long been known that the Pcp2(L7) mRNA is abundantly localized in dendrites, suggesting the possibility of distal synthesis and local changes in levels of the protein. As a first step to uncover the trafficking and translational mechanisms for this mRNA, we have begun identifying the cis-acting sequences important for its localization in dendrites. Using expression of modified transgenes in vivo, we show that the 3′UTR, only 65 bases long, is necessary in this process.

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These results were reported in part at a meeting: Serinagaoglu, Y., Iscru, E.M., Tian, J., Bishop, G.A., Morgan, J.I., Zhu, M.X., Oberdick, J. Sensorimotor behavioral changes and alteration of Purkinje cell firing patterns in Pcp2(L7) null mutant mice. Program No. 986.2. 2005 Abstract Viewer/Itinerary Planner. Washington, DC: Society for Neuroscience, 2005. Online.

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Titel: 3′UTR-Dependent Localization of a Purkinje Cell Messenger RNA in Dendrites
verfasst von: Rui Zhang
Xulun Zhang
Feng Bian
Xin-an Pu
Karl Schilling
John Oberdick
Publikationsdatum: 01.09.2008
Verlag: Springer-Verlag
Erschienen in: The Cerebellum / Ausgabe 3/2008
Print ISSN: 1473-4222
Elektronische ISSN: 1473-4230
DOI: https://doi.org/10.1007/s12311-008-0051-y

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