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01.12.2014 | Original Article

Dynamic pathology for circulating free DNA in a dextran sodium sulfate colitis mouse model

verfasst von: Yuhki Koike, Keiichi Uchida, Koji Tanaka, Shozo Ide, Kohei Otake, Yoshiki Okita, Mikihiro Inoue, Toshimitsu Araki, Akira Mizoguchi, Masato Kusunoki

Erschienen in: Pediatric Surgery International | Ausgabe 12/2014

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Abstract

Purpose

In sepsis, circulating free DNA (cf-DNA) is increased, and is a marker of severity and prognosis of septic patients. This study aimed to evaluate cf-DNA in a dextran sodium sulfate-induced colitis mouse model, and its clinical implications.

Methods

Dynamic pathology of the cecum wall in the DSS-induced colitis mouse model was analyzed using multiphoton microscopy (MPM). Plasma cf-DNA concentrations in colitis mouse were quantified using PicoGreen dsDNA Assay Kit. Plasma cf-DNA was also measured in 123 human ulcerative colitis (UC) patients [mean age: 35.9 years (3–75 years) with 20 pediatric patients] to assess its relationships with clinical severity and Matt’s grade.

Results

Real-time images of cf-DNA were detected in the colitis model. The amount of labeled cf-DNA in the circulation of the colitis mice group was significantly higher compared with that in the control group (P < 0.05). In human UC blood samples, plasma cf-DNA concentrations in UC patients were significantly positively correlated with the clinical severity of UC and Matt’s grade (P < 0.05, P < 0.05, respectively).

Conclusions

Using MPM, we observed and analyzed real-time images of cf-DNA in a colitis mouse model. Plasma cf-DNA is a potential non-invasive blood marker for reflecting clinical severity and mucosal damage in UC patients.

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Titel: Dynamic pathology for circulating free DNA in a dextran sodium sulfate colitis mouse model
verfasst von: Yuhki Koike
Keiichi Uchida
Koji Tanaka
Shozo Ide
Kohei Otake
Yoshiki Okita
Mikihiro Inoue
Toshimitsu Araki
Akira Mizoguchi
Masato Kusunoki
Publikationsdatum: 01.12.2014
Verlag: Springer Berlin Heidelberg
Erschienen in: Pediatric Surgery International / Ausgabe 12/2014
Print ISSN: 0179-0358
Elektronische ISSN: 1437-9813
DOI: https://doi.org/10.1007/s00383-014-3607-6

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Dynamic pathology for circulating free DNA in a dextran sodium sulfate colitis mouse model