Skip to main content
Erschienen in: International Journal of Legal Medicine 3/2007

01.05.2007 | Short Communication

Phylogenetic analysis of forensically important Lucilia flies based on cytochrome oxidase I sequence: a cautionary tale for forensic species determination

verfasst von: Jeffrey D. Wells, Richard Wall, Jamie R. Stevens

Erschienen in: International Journal of Legal Medicine | Ausgabe 3/2007

Einloggen, um Zugang zu erhalten

Abstract

Forensic scientists are increasingly using DNA to identify the species of a tissue sample. However, little attention has been paid to basic experimental design issues such as replication and the selection of taxa when designing a species diagnostic test. We present an example using the forensically important fly genus Lucilia in which an increasingly larger sample size revealed that species diagnosis based on the commonly used cytochrome oxidase I gene (COI) was less straightforward than we initially thought. This locus may still be useful for diagnosing Lucilia specimens, but additional knowledge other than the genotype will be required to reduce the list of candidate species to include only forms that can be distinguished by COI. We believe that these results illustrate the importance of study design and biological knowledge of the study species when proposing a DNA-based identification test for any taxonomic group.
Anhänge
Nur mit Berechtigung zugänglich
Literatur
1.
Zurück zum Zitat Amendt J, Campobasso CP, Gaudry E, Reiter C, LeBlanc HN, Hall MJR (2006) Best practice in forensic entomology—standards and guidelines. Int J Legal Med (in press). DOI 10.1007/s00414-006-0086-x Amendt J, Campobasso CP, Gaudry E, Reiter C, LeBlanc HN, Hall MJR (2006) Best practice in forensic entomology—standards and guidelines. Int J Legal Med (in press). DOI 10.​1007/​s00414-006-0086-x
2.
Zurück zum Zitat Aubertin D (1933) Revision of the genus Lucilia R.-D. (Diptera, Calliphoridae). Linn Soc J Zool 38:389–463 Aubertin D (1933) Revision of the genus Lucilia R.-D. (Diptera, Calliphoridae). Linn Soc J Zool 38:389–463
3.
Zurück zum Zitat Avise JC (2000) Phylogeography: the history and formation of species. Harvard University Press, Cambridge Avise JC (2000) Phylogeography: the history and formation of species. Harvard University Press, Cambridge
4.
Zurück zum Zitat Benecke M (1998) Random Amplified Polymorphic DNA (RAPD) typing of necrophageous insects (Diptera, Coleoptera) in criminal forensic studies: validation and use in praxi. Forensic Sci Int 98:157–168PubMedCrossRef Benecke M (1998) Random Amplified Polymorphic DNA (RAPD) typing of necrophageous insects (Diptera, Coleoptera) in criminal forensic studies: validation and use in praxi. Forensic Sci Int 98:157–168PubMedCrossRef
5.
Zurück zum Zitat Butler JM (2005) Forensic DNA typing. Elsevier, Amsterdam Butler JM (2005) Forensic DNA typing. Elsevier, Amsterdam
6.
Zurück zum Zitat Chen WY, Hung TH, Shiao SF (2004) Molecular identification of forensically important blow fly species (Diptera: Calliphoridae) in Taiwan. J Med Entomol 41:47–57PubMedCrossRef Chen WY, Hung TH, Shiao SF (2004) Molecular identification of forensically important blow fly species (Diptera: Calliphoridae) in Taiwan. J Med Entomol 41:47–57PubMedCrossRef
7.
Zurück zum Zitat Funk DJ, Omland KE (2003) Species-level paraphyly and polyphyly: frequency, causes, and consequences, with insights from animal mitochondrial DNA. Annu Rev Ecol Evol Syst 34:397–423CrossRef Funk DJ, Omland KE (2003) Species-level paraphyly and polyphyly: frequency, causes, and consequences, with insights from animal mitochondrial DNA. Annu Rev Ecol Evol Syst 34:397–423CrossRef
9.
Zurück zum Zitat Harvey ML, Dadour IR, Gaudieri S (2003) Mitochondrial DNA cytochrome oxidase I gene: potential for distinction between immature stages of some forensically important fly species (Diptera) in Western Australia. Forensic Sci Int 131:134–139PubMedCrossRef Harvey ML, Dadour IR, Gaudieri S (2003) Mitochondrial DNA cytochrome oxidase I gene: potential for distinction between immature stages of some forensically important fly species (Diptera) in Western Australia. Forensic Sci Int 131:134–139PubMedCrossRef
10.
Zurück zum Zitat Harvey ML, Mansell MW, Villet MH, Dadour IR (2003) Molecular identification of some forensically important blowflies of southern Africa and Australia. Med Vet Entomol 17:363–369PubMedCrossRef Harvey ML, Mansell MW, Villet MH, Dadour IR (2003) Molecular identification of some forensically important blowflies of southern Africa and Australia. Med Vet Entomol 17:363–369PubMedCrossRef
11.
12.
Zurück zum Zitat Lenstra JA (2003) DNA methods for identifying plant and animals species in foods. In: Lees M (ed) Food authenticity and traceability. CRC Press, Boca Raton, pp 34–53 Lenstra JA (2003) DNA methods for identifying plant and animals species in foods. In: Lees M (ed) Food authenticity and traceability. CRC Press, Boca Raton, pp 34–53
13.
Zurück zum Zitat Lunt DH, Zhang D-X, Szymura JM, Hewitt GM (1996) The insect cytochrome oxidase I gene: evolutionary patterns and conserved primers for phylogenetic studies. Insect Mol Biol 5:153–165PubMed Lunt DH, Zhang D-X, Szymura JM, Hewitt GM (1996) The insect cytochrome oxidase I gene: evolutionary patterns and conserved primers for phylogenetic studies. Insect Mol Biol 5:153–165PubMed
14.
Zurück zum Zitat Malgorn Y, Coquoz R (1999) DNA typing for identification of some species of Calliphoridae. An interest in forensic entomology. Forensic Sci Int 102:111–119PubMedCrossRef Malgorn Y, Coquoz R (1999) DNA typing for identification of some species of Calliphoridae. An interest in forensic entomology. Forensic Sci Int 102:111–119PubMedCrossRef
15.
Zurück zum Zitat Moritz C (1996) The uses of molecular phylogenies for conservation. In: Harvey PH, Leigh Brown AJ, Maynard Smith J, Nee S (eds) New uses for new phylogenies. Oxford University Press, Oxford, pp 203–214 Moritz C (1996) The uses of molecular phylogenies for conservation. In: Harvey PH, Leigh Brown AJ, Maynard Smith J, Nee S (eds) New uses for new phylogenies. Oxford University Press, Oxford, pp 203–214
16.
Zurück zum Zitat Norris KR (1990) Evidence for the multiple exotic origin of Australian populations of the sheep blowfly Lucilia cuprina (Wiedemann) (Diptera: Calliphoridae). Aust J Zool 38:635–648CrossRef Norris KR (1990) Evidence for the multiple exotic origin of Australian populations of the sheep blowfly Lucilia cuprina (Wiedemann) (Diptera: Calliphoridae). Aust J Zool 38:635–648CrossRef
17.
Zurück zum Zitat Palumbi SR, Cipriano F (1998) Species identification using genetic tools: the value of nuclear and mitochondrial gene sequences in whale conservation. J Heredity 89:459–464CrossRef Palumbi SR, Cipriano F (1998) Species identification using genetic tools: the value of nuclear and mitochondrial gene sequences in whale conservation. J Heredity 89:459–464CrossRef
18.
Zurück zum Zitat Palumbi SR, Cipriano F, Hare MP (2001) Predicting nuclear gene coalescence from mitochondrial data: the three-times rule. Evolution 55:859–868PubMedCrossRef Palumbi SR, Cipriano F, Hare MP (2001) Predicting nuclear gene coalescence from mitochondrial data: the three-times rule. Evolution 55:859–868PubMedCrossRef
19.
Zurück zum Zitat Ratcliffe ST, Webb DW, Weinzievr RA, Robertson HM (2003) PCR-RFLP identification of (Diptera, Calliphoridae) on human corpses. J Forensic Sci 48:783–785PubMed Ratcliffe ST, Webb DW, Weinzievr RA, Robertson HM (2003) PCR-RFLP identification of (Diptera, Calliphoridae) on human corpses. J Forensic Sci 48:783–785PubMed
20.
Zurück zum Zitat Smith KGV (1986) A manual of forensic entomology. British Museum (Natural History), London Smith KGV (1986) A manual of forensic entomology. British Museum (Natural History), London
21.
Zurück zum Zitat Sperling FAH, Anderson GS, Hickey DA (1994) A DNA-based approach to the identification of insect species used for postmortem interval estimation. J Forensic Sci 39:418–427 (published erratum appears in letter from Wells and Sperling (2000) J Forensic Sci 45:1358–1359)PubMed Sperling FAH, Anderson GS, Hickey DA (1994) A DNA-based approach to the identification of insect species used for postmortem interval estimation. J Forensic Sci 39:418–427 (published erratum appears in letter from Wells and Sperling (2000) J Forensic Sci 45:1358–1359)PubMed
22.
Zurück zum Zitat Stevens JR (2003) The evolution of myiasis in blowflies (Calliphoridae). Int J Parasitol 33:1105–1113PubMedCrossRef Stevens JR (2003) The evolution of myiasis in blowflies (Calliphoridae). Int J Parasitol 33:1105–1113PubMedCrossRef
23.
Zurück zum Zitat Stevens JR, Wall R (1995) The use of randomly amplified polymorphic DNA (RAPD) analysis for studies of genetic variation in populations of the blowfly Lucilia sericata (Diptera: Calliphoridae) in southern England. Bull Entomol Res 85:549–555CrossRef Stevens JR, Wall R (1995) The use of randomly amplified polymorphic DNA (RAPD) analysis for studies of genetic variation in populations of the blowfly Lucilia sericata (Diptera: Calliphoridae) in southern England. Bull Entomol Res 85:549–555CrossRef
24.
Zurück zum Zitat Stevens JR, Wall R (1996) Species, sub-species and hybrid populations of the blowflies Lucilia cuprina and Lucilia sericata (Diptera: Calliphoridae). Proc R Soc Lond B Biol Sci 263:1335–1341CrossRef Stevens JR, Wall R (1996) Species, sub-species and hybrid populations of the blowflies Lucilia cuprina and Lucilia sericata (Diptera: Calliphoridae). Proc R Soc Lond B Biol Sci 263:1335–1341CrossRef
25.
Zurück zum Zitat Stevens JR, Wall R (1997) The evolution of ectoparasitism in the genus Lucilia (Diptera: Calliphoridae). Int J Parasitol 27:51–59PubMedCrossRef Stevens JR, Wall R (1997) The evolution of ectoparasitism in the genus Lucilia (Diptera: Calliphoridae). Int J Parasitol 27:51–59PubMedCrossRef
26.
Zurück zum Zitat Stevens JR, Wall R (2001) Genetic relationships between blowflies (Calliphoridae) of forensic importance. Forensic Sci Int 120:116–123PubMedCrossRef Stevens JR, Wall R (2001) Genetic relationships between blowflies (Calliphoridae) of forensic importance. Forensic Sci Int 120:116–123PubMedCrossRef
27.
Zurück zum Zitat Stevens JR, Wall R, Wells JD (2002) Paraphyly in Hawaiian hybrid blowfly populations and the evolutionary history of anthropophilic species. Insect Mol Biol 11:141–148PubMedCrossRef Stevens JR, Wall R, Wells JD (2002) Paraphyly in Hawaiian hybrid blowfly populations and the evolutionary history of anthropophilic species. Insect Mol Biol 11:141–148PubMedCrossRef
28.
Zurück zum Zitat Swofford DL (1998) PAUP, phylogenetic analysis using parsimony (and other methods), version 4. Sinauer, Sunderland Swofford DL (1998) PAUP, phylogenetic analysis using parsimony (and other methods), version 4. Sinauer, Sunderland
29.
Zurück zum Zitat Vincent S, Vian JM, Carlotti MP (2000) Partial sequencing of the cytochrome oxydase b subunit gene I: a tool for the identification of European species of blow flies for postmortem interval estimation. J Forensic Sci 45:820–823 (published erratum appears in letter from Wells and Sperling (2000) J Forensic Sci 45:1358–1359)PubMed Vincent S, Vian JM, Carlotti MP (2000) Partial sequencing of the cytochrome oxydase b subunit gene I: a tool for the identification of European species of blow flies for postmortem interval estimation. J Forensic Sci 45:820–823 (published erratum appears in letter from Wells and Sperling (2000) J Forensic Sci 45:1358–1359)PubMed
30.
Zurück zum Zitat Wallman JF, Donnellan SC (2001) The utility of mitochondrial DNA sequences for the identification of forensically important blowflies (Diptera: Calliphoridae) in southeastern Australia. Forensic Sci Int 120:60–67PubMedCrossRef Wallman JF, Donnellan SC (2001) The utility of mitochondrial DNA sequences for the identification of forensically important blowflies (Diptera: Calliphoridae) in southeastern Australia. Forensic Sci Int 120:60–67PubMedCrossRef
31.
Zurück zum Zitat Wallman JF, Leys R, Hogendoorn K (2005) Molecular systematics of Australian carrion-breeding blowflies (Diptera: Calliphoridae) based on mitochondrial DNA. Invertebrate Systematics 19:1–15CrossRef Wallman JF, Leys R, Hogendoorn K (2005) Molecular systematics of Australian carrion-breeding blowflies (Diptera: Calliphoridae) based on mitochondrial DNA. Invertebrate Systematics 19:1–15CrossRef
32.
Zurück zum Zitat Ward J, Peakall R, Gilmore SR, Robertson J (2005) A molecular identification system for grasses: a novel technology for forensic botany. Forensic Sci Int 152:121–131PubMedCrossRef Ward J, Peakall R, Gilmore SR, Robertson J (2005) A molecular identification system for grasses: a novel technology for forensic botany. Forensic Sci Int 152:121–131PubMedCrossRef
33.
Zurück zum Zitat Waterhouse DF, Paramonov SJ (1950) The status of the two species of Lucilia (Diptera, Calliphoridae) attacking sheep in Australia. Aust J Sci Res B 3:310–336 Waterhouse DF, Paramonov SJ (1950) The status of the two species of Lucilia (Diptera, Calliphoridae) attacking sheep in Australia. Aust J Sci Res B 3:310–336
34.
Zurück zum Zitat Wells JD, Sperling FAH (1999) Molecular phylogeny of Chrysomya albiceps and C. rufifacies (Diptera: Calliphoridae). J Med Entomol 36:222–226PubMed Wells JD, Sperling FAH (1999) Molecular phylogeny of Chrysomya albiceps and C. rufifacies (Diptera: Calliphoridae). J Med Entomol 36:222–226PubMed
35.
Zurück zum Zitat Wells JD, Sperling FAH (2001) DNA-based identification of forensically important Chrysomyinae (Diptera: Calliphoridae). Forensic Sci Int 120:109–114CrossRef Wells JD, Sperling FAH (2001) DNA-based identification of forensically important Chrysomyinae (Diptera: Calliphoridae). Forensic Sci Int 120:109–114CrossRef
36.
Zurück zum Zitat Wells JD, Williams DW (2007) Validation of a DNA-based method for identifying Chrysomyinae (Diptera: Calliphoridae) used in a death investigation. Int J Legal Med 121:1–8 Wells JD, Williams DW (2007) Validation of a DNA-based method for identifying Chrysomyinae (Diptera: Calliphoridae) used in a death investigation. Int J Legal Med 121:1–8
37.
Zurück zum Zitat Wells JD, Introna F, Di Vella G, Campobasso CP, Hayes J, Sperling FA (2001) Human and insect mitochondrial DNA analysis from maggots. J Forensic Sci 46:261–263 Wells JD, Introna F, Di Vella G, Campobasso CP, Hayes J, Sperling FA (2001) Human and insect mitochondrial DNA analysis from maggots. J Forensic Sci 46:261–263
38.
Zurück zum Zitat Wells JD, Goff ML, Tomberlin JK, Kurahashi H (2002) Molecular systematics of the endemic Hawaiian blowfly genus Dyscritomyia Grimshaw, (Diptera: Calliphoridae). Med Entomol Zool 53(Suppl 2):231–238 Wells JD, Goff ML, Tomberlin JK, Kurahashi H (2002) Molecular systematics of the endemic Hawaiian blowfly genus Dyscritomyia Grimshaw, (Diptera: Calliphoridae). Med Entomol Zool 53(Suppl 2):231–238
39.
Zurück zum Zitat Zehner R, Amendt J, Schutt S, Sauer J, Krettek R, Povolny D (2004) Genetic identification of forensically important flesh flies (Diptera: Sarcophagidae). Int J Legal Med 118: 245–247PubMedCrossRef Zehner R, Amendt J, Schutt S, Sauer J, Krettek R, Povolny D (2004) Genetic identification of forensically important flesh flies (Diptera: Sarcophagidae). Int J Legal Med 118: 245–247PubMedCrossRef
Metadaten
Titel
Phylogenetic analysis of forensically important Lucilia flies based on cytochrome oxidase I sequence: a cautionary tale for forensic species determination
verfasst von
Jeffrey D. Wells
Richard Wall
Jamie R. Stevens
Publikationsdatum
01.05.2007
Verlag
Springer-Verlag
Erschienen in
International Journal of Legal Medicine / Ausgabe 3/2007
Print ISSN: 0937-9827
Elektronische ISSN: 1437-1596
DOI
https://doi.org/10.1007/s00414-006-0147-1

Weitere Artikel der Ausgabe 3/2007

International Journal of Legal Medicine 3/2007 Zur Ausgabe

Neu im Fachgebiet Rechtsmedizin

Assistierter Suizid durch Infusion von Thiopental

Thiopental Originalie

Als Folge des Urteils des Bundesverfassungsgerichts zur Sterbehilfe im Jahr 2020 wurde in den Jahren 2021–2023 eine Reihe (n = 23) von assistierten Suiziden im Landesinstitut für gerichtliche und soziale Medizin Berlin mit jeweils identischen …

Molekularpathologische Untersuchungen im Wandel der Zeit

Open Access Biomarker Leitthema

Um auch an kleinen Gewebeproben zuverlässige und reproduzierbare Ergebnisse zu gewährleisten ist eine strenge Qualitätskontrolle in jedem Schritt des Arbeitsablaufs erforderlich. Eine nicht ordnungsgemäße Prüfung oder Behandlung des …

Vergleichende Pathologie in der onkologischen Forschung

Pathologie Leitthema

Die vergleichende experimentelle Pathologie („comparative experimental pathology“) ist ein Fachbereich an der Schnittstelle von Human- und Veterinärmedizin. Sie widmet sich der vergleichenden Erforschung von Gemeinsamkeiten und Unterschieden von …

Gastrointestinale Stromatumoren

Open Access GIST CME-Artikel

Gastrointestinale Stromatumoren (GIST) stellen seit über 20 Jahren ein Paradigma für die zielgerichtete Therapie mit Tyrosinkinaseinhibitoren dar. Eine elementare Voraussetzung für eine mögliche neoadjuvante oder adjuvante Behandlung bei …