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Forensic Toxicology

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01.07.2019 | Original Article

Species identification of white false hellebore (Veratrum album subsp. oxysepalum) by loop-mediated isothermal amplification (LAMP)

verfasst von: Hitomi S. Kikkawa, Masako Aragane, Kouichiro Tsuge

Erschienen in: Forensic Toxicology | Ausgabe 2/2019

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Abstract

Purpose

Food poisoning is frequently caused by the accidental ingestion of toxic plants with strong morphological similarities to edible plants. The plant, false helleborine (Veratrum album), is commonly involved in such incidents. Cases of poisoning by toxic plants require rapid and accurate identification for appropriate medical treatment or forensic investigation in the event of death. However, morphological examinations require experience in systematic botany, and chemical analysis of natural compounds can be challenging. This study aimed to develop a sensitive, rapid, and specific method for identifying V. album and V. stamineum.

Methods

We developed and optimized a loop-mediated isothermal amplification (LAMP) detection method. Total genomic DNA was extracted from approximately 10 mg (dry weight) of leaf from 26 specimens comprising the target plants, allied plants, and other causative plants of poisoning. These samples were analyzed the LAMP assay, and mixed samples, cooked samples, and simulated gastric contents were also tested.

Results

The limit of detection of our LAMP assay was 0.08 ng of target DNA, and the assay was specific to V. album and V. stamineum. The LAMP assay could be performed in approximately 30 min. V. album was also successfully identified in mixed samples, cooked samples, and simulated gastric contents. In addition, the LAMP assay signal could be effectively assessed with the naked eye.

Conclusions

The present LAMP assay is a highly sensitive, rapid, and specific method for identifying V. album and V. stamineum.

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Titel: Species identification of white false hellebore (Veratrum album subsp. oxysepalum) by loop-mediated isothermal amplification (LAMP)
verfasst von: Hitomi S. Kikkawa
Masako Aragane
Kouichiro Tsuge
Publikationsdatum: 01.07.2019
Verlag: Springer Singapore
Erschienen in: Forensic Toxicology / Ausgabe 2/2019
Print ISSN: 1860-8965
Elektronische ISSN: 1860-8973
DOI: https://doi.org/10.1007/s11419-018-00461-y

Springer Medizin

Species identification of white false hellebore (Veratrum album subsp. oxysepalum) by loop-mediated isothermal amplification (LAMP)

Abstract

Purpose

Methods

Results

Conclusions

Neu im Fachgebiet Rechtsmedizin

Molekularpathologische Untersuchungen im Wandel der Zeit

Vergleichende Pathologie in der onkologischen Forschung

Gastrointestinale Stromatumoren

Personalisierte Medizin in der Onkologie

Springer Medizin

Abstract

Purpose

Methods

Results

Conclusions

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