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30.05.2018 | Methods Paper

Technical note: developmental validation of a novel 6-dye typing system with 36 Y-STR loci

verfasst von: Weian Du, Peipei Feng, Hongyan Huang, Weibin Wu, Lei Zhang, Yulin Guo, Changhui Liu, Hong Liu, Chao Liu, Ling Chen

Erschienen in: International Journal of Legal Medicine | Ausgabe 4/2019

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Abstract

Y-chromosomal short tandem repeats (Y-STRs) have proven to be very useful in investigating sexual assault cases and in paternity lineage differentiation. However, currently available commercial Y-STR multiplex amplification systems bear the limitations in the identification of related males from the same paternal lineage due to there being an insufficient number of loci in any single amplification kit. The aim of this study was to establish and validate a novel 6-dye, 36-plex Y-STR multiplex amplification system that incorporated all of the loci present in the Yfiler™ Plus kit (DYS19, DYS385a/b, DYF387S1, DYS389I/II, DYS390, DYS391, DYS392, DYS393, DYS437, DYS438, DYS439, DYS448, DYS449, DYS456, DYS458, DYS460, DYS481, DYS518, DYS533, DYS570, DYS576, DYS627, DYS635, Y_GATA_H4) as well as a further nine highly polymorphic Y-STR loci (DYS388, DYS444, DYS447, DYS522, DYS527a/b, DYS549, DYS596, DYS643). The novel system was optimized and validated by a series of studies that tested the effect of different PCR-based conditions as well as the species specificity, sensitivity, stability, stutter precision, suitability for use on DNA mixtures, reproducibility, and parallel testing of the system, as well as its performance on casework samples and population analysis, according to the SWGDAM developmental validation guidelines. A total of 246 haplotypes were found for the 36 Y-STRs among 247 Guangdong Han unrelated males. Collectively, the results demonstrate that the developed 36-plex Y-STR system is sensitive, robust, reliable, and highly informative for use in forensic genetics.

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Titel: Technical note: developmental validation of a novel 6-dye typing system with 36 Y-STR loci
verfasst von: Weian Du
Peipei Feng
Hongyan Huang
Weibin Wu
Lei Zhang
Yulin Guo
Changhui Liu
Hong Liu
Chao Liu
Ling Chen
Publikationsdatum: 30.05.2018
Verlag: Springer Berlin Heidelberg
Erschienen in: International Journal of Legal Medicine / Ausgabe 4/2019
Print ISSN: 0937-9827
Elektronische ISSN: 1437-1596
DOI: https://doi.org/10.1007/s00414-018-1864-y

Springer Medizin

Technical note: developmental validation of a novel 6-dye typing system with 36 Y-STR loci

Abstract

Neu im Fachgebiet Rechtsmedizin

Molekularpathologische Untersuchungen im Wandel der Zeit

Vergleichende Pathologie in der onkologischen Forschung

Gastrointestinale Stromatumoren

Personalisierte Medizin in der Onkologie

Springer Medizin

Abstract

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