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Journal of Cardiovascular Translational Research
Erschienen in: Journal of Cardiovascular Translational Research 5/2018

19.09.2018 | Original Article

Transplantation of Cardiac Mesenchymal Stem Cell-Derived Exosomes Promotes Repair in Ischemic Myocardium

verfasst von: Chengwei Ju, Yan Shen, Gengshan Ma, Yutao Liu, Jingwen Cai, Il-man Kim, Neal L. Weintraub, Naifeng Liu, Yaoliang Tang

Erschienen in: Journal of Cardiovascular Translational Research | Ausgabe 5/2018

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Abstract

Our previous study demonstrated the beneficial effects of exosomes secreted by cardiac mesenchymal stem cells (C-MSC-Exo) in protecting acute ischemic myocardium from reperfusion injury. Here, we investigated the effect of exosomes from C-MSC on angiogenesis in ischemic myocardium. We intramyocardially injected C-MSC-Exo or PBS into the infarct border zone after induction of acute mouse myocardial infarction (MI). We observed that hearts treated with C-MSC-Exo exhibit improved cardiac function compared to control hearts treated with PBS at one month after MI. Capillary density and Ki67-postive cells were significantly higher following treatment with C-MSC-Exo as compared with PBS. Moreover, C-MSC-Exo treatment increased cardiomyocyte proliferation in infarcted hearts. In conclusion, intramyocardial delivery of C-MSC-Exo after myocardial infarction enhances cardiac angiogenesis, promotes cardiomyocyte proliferation, and preserves heart function. C-MSC-Exo constitute a novel form of cell-free therapy for cardiac repair.
Literatur
1.
Roth, G. A., Huffman, M. D., Moran, A. E., Feigin, V., Mensah, G. A., Naghavi, M., et al. (2015). Global and regional patterns in cardiovascular mortality from 1990 to 2013. Circulation, 132(17), 1667–1678.CrossRef
2.
Porrello, E. R., Mahmoud, A. I., Simpson, E., Johnson, B. A., Grinsfelder, D., Canseco, D., et al. (2013). Regulation of neonatal and adult mammalian heart regeneration by the miR-15 family. Proceedings of the National Academy of Sciences of the United States of America, 110(1), 187–192.CrossRef
3.
Konstam, M. A., Kramer, D. G., Patel, A. R., Maron, M. S., & Udelson, J. E. (2011). Left ventricular remodeling in heart failure: current concepts in clinical significance and assessment. JACC Cardiovascular imaging., 4(1), 98–108.CrossRef
4.
Wang Z, Su X, Ashraf M, Kim IM, Weintraub NL, Jiang M, et al. Regenerative therapy for cardiomyopathies. Journal of Cardiovascular Translational Research. 2018.
5.
Djohan AH, Sia CH, Lee PS, Poh KK. Endothelial progenitor cells in heart failure: an authentic expectation for potential future use and a lack of universal definition. Journal of Cardiovascular Translational Research. 2018.
6.
Hagan, M., Ashraf, M., Kim, I. M., Weintraub, N. L., & Tang, Y. (2018). Effective regeneration of dystrophic muscle using autologous iPSC-derived progenitors with CRISPR-Cas9 mediated precise correction. Medical Hypotheses, 110, 97–100.CrossRef
7.
Bolli, R., Chugh, A. R., D'Amario, D., Loughran, J. H., Stoddard, M. F., Ikram, S., et al. (2011). Cardiac stem cells in patients with ischaemic cardiomyopathy (SCIPIO): initial results of a randomised phase 1 trial. Lancet, 378(9806), 1847–1857.CrossRef
8.
Makkar, R. R., Smith, R. R., Cheng, K., Malliaras, K., Thomson, L. E., Berman, D., et al. (2012). Intracoronary cardiosphere-derived cells for heart regeneration after myocardial infarction (CADUCEUS): a prospective, randomised phase 1 trial. Lancet, 379(9819), 895–904.CrossRef
9.
Mirotsou, M., Jayawardena, T. M., Schmeckpeper, J., Gnecchi, M., & Dzau, V. J. (2011). Paracrine mechanisms of stem cell reparative and regenerative actions in the heart. Journal of Molecular And Cellular Cardiology, 50(2), 280–289.CrossRef
10.
Burchfield, J. S., & Dimmeler, S. (2008). Role of paracrine factors in stem and progenitor cell mediated cardiac repair and tissue fibrosis. Fibrogenesis & Tissue Repair, 1(1), 4.CrossRef
11.
Ruan, X. F., Li, Y. J., Ju, C. W., Shen, Y., Lei, W., Chen, C., et al. (2018). Exosomes from Suxiao Jiuxin pill-treated cardiac mesenchymal stem cells decrease H3K27 demethylase UTX expression in mouse cardiomyocytes in vitro. Acta Pharmacologica Sinica, 39(4), 579–586.CrossRef
12.
Chen, L., Wang, Y., Pan, Y., Zhang, L., Shen, C., Qin, G., et al. (2013). Cardiac progenitor-derived exosomes protect ischemic myocardium from acute ischemia/reperfusion injury. Biochemical and Biophysical Research Communications, 431(3), 566–571.CrossRef
13.
Tang, Y. L., Zhao, Q., Zhang, Y. C., Cheng, L., Liu, M., Shi, J., et al. (2004). Autologous mesenchymal stem cell transplantation induce VEGF and neovascularization in ischemic myocardium. Regulatory peptides, 117(1), 3–10.CrossRef
14.
Tang, Y. L., Zhao, Q., Qin, X., Shen, L., Cheng, L., Ge, J., et al. (2005). Paracrine action enhances the effects of autologous mesenchymal stem cell transplantation on vascular regeneration in rat model of myocardial infarction. The Annals of Thoracic Surgery, 80(1), 229–236 discussion 36-7.CrossRef
15.
Sahoo, S., Klychko, E., Thorne, T., Misener, S., Schultz, K. M., Millay, M., et al. (2011). Exosomes from human CD34(+) stem cells mediate their proangiogenic paracrine activity. Circulation Research., 109(7), 724–728.CrossRef
16.
Mathiyalagan, P., Liang, Y., Kim, D., Misener, S., Thorne, T., Kamide, C. E., et al. (2017). Angiogenic mechanisms of human CD34(+) stem cell exosomes in the repair of ischemic hindlimb. Circulation research., 120(9), 1466–1476.CrossRef
17.
Khan, M., & Kishore, R. (2017). Stem cell exosomes: cell-freetherapy for organ repair. Methods in molecular biology (Clifton, NJ), 1553, 315–321.CrossRef
18.
Ruan, X. F., Ju, C. W., Shen, Y., Liu, Y. T., Kim, I. M., Yu, H., et al. (2018). Suxiao Jiuxin pill promotes exosome secretion from mouse cardiac mesenchymal stem cells in vitro. Acta Pharmacologica Sinica, 39(4), 569–578.CrossRef
19.
Ni J, Sun Y, Liu Z. The potential of stem cells and stem cell-derived exosomes in treating cardiovascular diseases. Journal of Cardiovascular Translational Research. 2018.
20.
Sagini K, Costanzi E, Emiliani C, Buratta S, Urbanelli L. Extracellular vesicles as conveyors of membrane-derived bioactive lipids in immune system. International journal of molecular sciences. 2018;19(4).CrossRef
21.
Lu M, Yuan S, Li S, Li L, Liu M, Wan S. The exosome-derived biomarker in atherosclerosis and its clinical application. Journal of Cardiovascular Translational Research. 2018.
22.
McBride, J. D., Rodriguez-Menocal, L., Guzman, W., Candanedo, A., Garcia-Contreras, M., & Badiavas, E. V. (2017). Bone marrow mesenchymal stem cell-derived CD63(+) exosomes transport Wnt3a exteriorly and enhance dermal fibroblast proliferation, migration, and angiogenesis in vitro. Stem Cells and Development., 26(19), 1384–1398.CrossRef
23.
Ha, D., Yang, N., & Nadithe, V. (2016). Exosomes as therapeutic drug carriers and delivery vehicles across biological membranes: current perspectives and future challenges. Acta Pharmaceutica Sinica B., 6(4), 287–296.CrossRef
24.
Liu X, Yuan W, Yang L, Li J, Cai J. miRNA profiling of exosomes from spontaneous hypertensive rats using next-generation sequencing. Journal of Cardiovascular Translational Research. 2018.
25.
Sun, Z., Yang, S., Zhou, Q., Wang, G., Song, J., Li, Z., et al. (2018). Emerging role of exosome-derived long non-coding RNAs in tumor microenvironment. Molecular cancer., 17(1), 82.CrossRef
26.
Hagan, M., Zhou, M., Ashraf, M., Kim, I. M., Su, H., Weintraub, N. L., et al. (2017). Long noncoding RNAs and their roles in skeletal muscle fate determination. Non-coding RNA investigation., 1.CrossRef
27.
Mayourian, J., Ceholski, D. K., Gorski, P. A., Mathiyalagan, P., Murphy, J. F., Salazar, S. I., et al. (2018). Exosomal microRNA-21-5p mediates mesenchymal stem cell paracrine effects on human cardiac tissue contractility. Circulation Research., 122(7), 933–944.CrossRef
28.
Mathiyalagan, P., & Sahoo, S. (2017). Exosomes-based gene therapy for MicroRNA delivery. Methods in molecular biology (Clifton, NJ), 1521, 139–152.CrossRef
29.
Wang, Y., Zhang, L., Li, Y., Chen, L., Wang, X., Guo, W., et al. (2015). Exosomes/microvesicles from induced pluripotent stem cells deliver cardioprotective miRNAs and prevent cardiomyocyte apoptosis in the ischemic myocardium. International Journal of Cardiology., 192, 61–69.CrossRef
30.
Bian, S., Zhang, L., Duan, L., Wang, X., Min, Y., & Yu, H. (2014). Extracellular vesicles derived from human bone marrow mesenchymal stem cells promote angiogenesis in a rat myocardial infarction model. Journal of molecular medicine (Berlin, Germany), 92(4), 387–397.CrossRef
31.
Luther KM, Haar L, McGuinness M, Wang Y, Lynch T, Phan A, et al. Exosomal miR-21a-5p mediates cardioprotection by mesenchymal stem cells. Journal of Molecular and Cellular Cardiology. 2018.
32.
Vrijsen, K. R., Maring, J. A., Chamuleau, S. A., Verhage, V., Mol, E. A., Deddens, J. C., et al. (2016). Exosomes from cardiomyocyte progenitor cells and mesenchymal stem cells stimulate angiogenesis via EMMPRIN. Advanced healthcare materials., 5(19), 2555–2565.CrossRef
33.
Chen, L., Pan, Y., Zhang, L., Wang, Y., Weintraub, N., & Tang, Y. (2013). Two-step protocol for isolation and culture of cardiospheres. Methods in molecular biology (Clifton, NJ), 1036, 75–80.CrossRef
34.
Chen, Z., Li, Y., Yu, H., Shen, Y., Ju, C., Ma, G., et al. (2017). Isolation of extracellular vesicles from stem cells. Methods in molecular biology (Clifton, NJ), 1660, 389–394.CrossRef
35.
Helwa, I., Cai, J., Drewry, M. D., Zimmerman, A., Dinkins, M. B., Khaled, M. L., et al. (2017). A comparative study of serum exosome isolation using differential ultracentrifugation and three commercial reagents. PLoS One, 12(1), e0170628.CrossRef
36.
Gao, L., Gregorich, Z. R., Zhu, W., Mattapally, S., Oduk, Y., Lou, X., et al. (2018). Large cardiac muscle patches engineered from human induced-pluripotent stem cell-derived cardiac cells improve recovery from myocardial infarction in swine. Circulation, 137(16), 1712–1730.CrossRef
37.
Zhang, L., Zhou, M., Qin, G., Weintraub, N. L., & Tang, Y. (2014). MiR-92a regulates viability and angiogenesis of endothelial cells under oxidative stress. Biochemical and biophysical research communications., 446(4), 952–958.CrossRef
38.
Chen L, Phillips MI, Miao HL, Zeng R, Qin G, Kim IM, et al. Infrared fluorescent protein 1.4 genetic labeling tracks engrafted cardiac progenitor cells in mouse ischemic hearts. PloS one. 2014;9(10):e107841.CrossRef
39.
Wang, Y., Zhou, M., Wang, X., Qin, G., Weintraub, N. L., & Tang, Y. (2014). Assessing in vitro stem-cell function and tracking engraftment of stem cells in ischaemic hearts by using novel iRFP gene labelling. Journal of cellular and molecular medicine., 18(9), 1889–1894.CrossRef
40.
Tang, Y. L., Tang, Y., Zhang, Y. C., Qian, K., Shen, L., & Phillips, M. I. (2005). Improved graft mesenchymal stem cell survival in ischemic heart with a hypoxia-regulated heme oxygenase-1 vector. Journal of the American College of Cardiology., 46(7), 1339–1350.CrossRef
41.
Ruan XF, Ju CW, Shen Y, Liu YT, Kim IM, Yu H, et al. Suxiao Jiuxin pill promotes exosome secretion from mouse cardiac mesenchymal stem cells in vitro. Acta pharmacologica Sinica. 2018.
42.
Kim, S. W., Houge, M., Brown, M., Davis, M. E., & Yoon, Y. S. (2014). Cultured human bone marrow-derived CD31(+) cells are effective for cardiac and vascular repair through enhanced angiogenic, adhesion, and anti-inflammatory effects. Journal of the American College of Cardiology., 64(16), 1681–1694.CrossRef
43.
Timmers, L., Lim, S. K., Hoefer, I. E., Arslan, F., Lai, R. C., van Oorschot, A. A., et al. (2011). Human mesenchymal stem cell-conditioned medium improves cardiac function following myocardial infarction. Stem cell research., 6(3), 206–214.CrossRef
44.
Amin, S., Banijamali, S. E., Tafazoli-Shadpour, M., Shokrgozar, M. A., Dehghan, M. M., Haghighipour, N., et al. (2014). Comparing the effect of equiaxial cyclic mechanical stimulation on GATA4 expression in adipose-derived and bone marrow-derived mesenchymal stem cells. Cell biology international., 38(2), 219–227.CrossRef
45.
Yu, B., Kim, H. W., Gong, M., Wang, J., Millard, R. W., Wang, Y., et al. (2015). Exosomes secreted from GATA-4 overexpressing mesenchymal stem cells serve as a reservoir of anti-apoptotic microRNAs for cardioprotection. International journal of cardiology., 182, 349–360.CrossRef
46.
Figeac F, Lesault PF, Le Coz O, Damy T, Souktani R, Trebeau C, et al. Nanotubular crosstalk with distressed cardiomyocytes stimulates the paracrine repair function of mesenchymal stem cells. Stem cells (Dayton, Ohio). 2014;32(1):216–30.CrossRef
47.
Shao, L., Zhang, Y., Lan, B., Wang, J., Zhang, Z., Zhang, L., et al. (2017). MiRNA-sequence indicates that mesenchymal stem cells and exosomes have similar mechanism to enhance cardiac repair. BioMed research international., 2017, 4150705.PubMedPubMedCentral
48.
Khan, M., Nickoloff, E., Abramova, T., Johnson, J., Verma, S. K., Krishnamurthy, P., et al. (2015). Embryonic stem cell-derived exosomes promote endogenous repair mechanisms and enhance cardiac function following myocardial infarction. Circulation research., 117(1), 52–64.CrossRef
49.
Li, P., Liu, Z., Xie, Y., Gu, H., Dai, Q., Yao, J., et al. (2018). Serum exosomes attenuate H2O2-induced apoptosis in rat H9C2 cardiomyocytes via ERK1/2. Journal of cardiovascular translational research.
50.
Campbell, C. R., Berman, A. E., Weintraub, N. L., & Tang, Y. L. (2016). Electrical stimulation to optimize cardioprotective exosomes from cardiac stem cells. Medical hypotheses., 88, 6–9.CrossRef
Metadaten
Titel
Transplantation of Cardiac Mesenchymal Stem Cell-Derived Exosomes Promotes Repair in Ischemic Myocardium
verfasst von
Chengwei Ju
Yan Shen
Gengshan Ma
Yutao Liu
Jingwen Cai
Il-man Kim
Neal L. Weintraub
Naifeng Liu
Yaoliang Tang
Publikationsdatum
19.09.2018
Verlag
Springer US
Erschienen in
Journal of Cardiovascular Translational Research / Ausgabe 5/2018
Print ISSN: 1937-5387
Elektronische ISSN: 1937-5395
DOI
https://doi.org/10.1007/s12265-018-9822-0

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