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Archives of Virology

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04.07.2016 | Original Article

The Vibrio parahaemolyticus-infecting bacteriophage qdvp001: genome sequence and endolysin with a modular structure

verfasst von: Weiyu Wang, Mengzhe Li, Hong Lin, Jingxue Wang, Xiangzhao Mao

Erschienen in: Archives of Virology | Ausgabe 10/2016

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Abstract

Vibrio parahaemolyticus, a marine pathogen, is a causative agent of gastroenteritis in humans after consumption of contaminated seafood. In recent years, infections with V. parahaemolyticus have become an increasingly frequent factor in microbial food poisoning; therefore, it is urgent to figure out ways to control Vibrio parahaemolyticus. Endolysins, lytic enzymes encoded by bacteriophages, have been regarded as a therapeutic alternative to antibiotics in control of bacterial growth and have been successfully utilized in various areas. Here, we report the full genome sequence of the novel phage qdvp001, which lyses Vibrio parahaemolyticus 17802. The qdvp001 genome consists of a 134,742-bp DNA with a G+C content of 35.35 % and 227 putative open reading frames. Analysis revealed that the qdvp001 open reading frames encoded various putative functional proteins with a putative endolysin gene (ORF 60). No holin genes were identified in qdvp001. ORF 60 was cloned and expressed. The results showed that the purified endolysin Lysqdvp001 had a high hydrolytic activity toward Vibrio parahaemolyticus and a broader spectrum compared to that of the parental bacteriophage qdvp001. Thus, purified endolysin Lysqdvp001 has a potential to be used as an antibacterial agent in the future.

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Su YC, Liu C (2007) Vibrio parahaemolyticus: a concern of seafood safety. Food Microbiol 24(6):549–558CrossRefPubMed

Fuenzalida L et al (2007) Vibrio parahaemolyticus strains isolated during investigation of the summer 2006 seafood related diarrhea outbreaks in two regions of Chile. Int J Food Microbiol 117(3):270–275CrossRefPubMed

Oh EG et al (2011) Antimicrobial resistance of Vibrio parahaemolyticus and Vibrio alginolyticus strains isolated from farmed fish in Korea from 2005 through 2007. J Food Prot 74(3):380–386CrossRefPubMed

Schmelcher M, Donovan DM, Loessner MJ (2012) Bacteriophage endolysins as novel antimicrobials. Future Microbiol 7(10):1147–1171CrossRefPubMedPubMedCentral

Courchesne NM, Parisien A, Lan CQ (2009) Production and application of bacteriophage and bacteriophage-encoded lysins. Recent Pat Biotechnol 3(1):37–45CrossRefPubMed

Gupta R, Prasad Y (2011) P-27/HP endolysin as antibacterial agent for antibiotic resistant Staphylococcus aureus of human infections. Curr Microbiol 63(1):39–45CrossRefPubMed

Fukushima T et al (2007) Characterization of new L, D-endopeptidase gene product CwlK (previous YcdD) that hydrolyzes peptidoglycan in Bacillus subtilis. Mol Genet Genomics 278(4):371–383CrossRefPubMed

Kikkawa H et al (2007) Identification of the amino acid residues critical for specific binding of the bacteriolytic enzyme of gamma-phage, PlyG, to Bacillus anthracis. Biochem Biophys Res Commun 363(3):531–535CrossRefPubMed

Loeffler JM, Djurkovic S, Fischetti VA (2003) Phage lytic enzyme Cpl-1 as a novel antimicrobial for pneumococcal bacteremia. Infect Immun 71(11):6199–6204CrossRefPubMedPubMedCentral

10.

Nariya H et al (2011) Identification and characterization of a putative endolysin encoded by episomal phage phiSM101 of Clostridium perfringens. Appl Microbiol Biotechnol 90(6):1973–1979CrossRefPubMed

11.

Sass P, Bierbaum G (2007) Lytic activity of recombinant bacteriophage phi11 and phi12 endolysins on whole cells and biofilms of Staphylococcus aureus. Appl Environ Microbiol 73(1):347–352CrossRefPubMed

12.

Schuch R, Nelson D, Fischetti VA (2002) A bacteriolytic agent that detects and kills Bacillus anthracis. Nature 418(6900):884–889CrossRefPubMed

13.

Turner MS et al (2004) Identification, characterisation and specificity of a cell wall lytic enzyme from Lactobacillus fermentum BR11. FEMS Microbiol Lett 238(1):9–15PubMed

14.

Turner MS et al (2007) Antimicrobial activity of lysostaphin and a Listeria monocytogenes bacteriophage endolysin produced and secreted by lactic acid bacteria. Syst Appl Microbiol 30(1):58–67CrossRefPubMed

15.

Uchiyama J et al (2011) Characterization of lytic enzyme open reading frame 9 (ORF9) derived from Enterococcus faecalis bacteriophage phiEF24C. Appl Environ Microbiol 77(2):580–585CrossRefPubMed

16.

Fischetti VA (2010) Bacteriophage endolysins: a novel anti-infective to control Gram-positive pathogens. Int J Med Microbiol 300(6):357–362CrossRefPubMedPubMedCentral

17.

Briers Y et al (2007) Muralytic activity and modular structure of the endolysins of Pseudomonas aeruginosa bacteriophages phiKZ and EL. Mol Microbiol 65(5):1334–1344CrossRefPubMed

18.

Junn HJ et al (2005) Cloning and expression of Klebsiella phage K11 lysozyme gene. Protein Expr Purif 42(1):78–84CrossRefPubMed

19.

Lai MJ et al (2011) Antibacterial activity of Acinetobacter baumannii phage varphiAB2 endolysin (LysAB2) against both gram-positive and gram-negative bacteria. Appl Microbiol Biotechnol 90(2):529–539CrossRefPubMed

20.

Mikoulinskaia GV et al (2009) Identification and characterization of the metal ion-dependent L-alanoyl-D-glutamate peptidase encoded by bacteriophage T5. FEBS J 276(24):7329–7342CrossRefPubMed

21.

Miroshnikov KA et al (2006) Properties of the endolytic transglycosylase encoded by gene 144 of Pseudomonas aeruginosa bacteriophage phiKZ. Biochem (Mosc) 71(3):300–305CrossRef

22.

Paradis-Bleau C et al (2007) Peptidoglycan lytic activity of the Pseudomonas aeruginosa phage phiKZ gp144 lytic transglycosylase. FEMS Microbiol Lett 266(2):201–209CrossRefPubMed

23.

Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254CrossRefPubMed

24.

Nelson D, Loomis L, Fischetti VA (2001) Prevention and elimination of upper respiratory colonization of mice by group A streptococci by using a bacteriophage lytic enzyme. Proc Natl Acad Sci USA 98(7):4107–4112CrossRefPubMedPubMedCentral

25.

Li P et al (2012) Bioinformatic analysis of the Acinetobacter baumannii phage AB1 genome. Gene 507(2):125–134CrossRefPubMed

26.

Newcomb WW, Homa FL, Brown JC (2005) Involvement of the portal at an early step in herpes simplex virus capsid assembly. J Virol 79(16):10540–10546CrossRefPubMedPubMedCentral

27.

Fischetti VA (2008) Bacteriophage lysins as effective antibacterials. Curr Opin Microbiol 11(5):393–400CrossRefPubMedPubMedCentral

28.

Bickle TA, Kruger DH (1993) Biology of DNA restriction. Microbiol Rev 57(2):434–450PubMedPubMedCentral

29.

Hill C, Miller LA, Klaenhammer TR (1991) In vivo genetic exchange of a functional domain from a type II A methylase between lactococcal plasmid pTR2030 and a virulent bacteriophage. J Bacteriol 173(14):4363–4370PubMedPubMedCentral

30.

Tang W et al (2000) The cause of deviation made in determing the molecular weight of His-tag fusion proteins by SDS-PAGE, pp 64–68

31.

Sillankorva S et al (2011) Efficacy of a broad host range lytic bacteriophage against E. coli adhered to urothelium. Curr Microbiol 62(4):1128–1132CrossRefPubMed

32.

Young I, Wang I, Roof WD (2000) Phages will out: strategies of host cell lysis. Trends Microbiol 8(3):120–128CrossRefPubMed

33.

Loessner MJ (2005) Bacteriophage endolysins–current state of research and applications. Curr Opin Microbiol 8(4):480–487CrossRefPubMed

34.

Callewaert L et al (2011) Food applications of bacterial cell wall hydrolases. Curr Opin Biotechnol 22(2):164–171CrossRefPubMed

35.

Fischetti VA (2010) Bacteriophage endolysins: a novel anti-infective to control Gram-positive pathogens. Int J Med Microbiol 300(6):357–362CrossRefPubMedPubMedCentral

36.

Briers Y, Lavigne R (2015) Breaking barriers: expansion of the use of endolysins as novel antibacterials against Gram-negative bacteria. Future Microbiol 10(3):377–390CrossRefPubMed

37.

Pastagia M et al (2013) Lysins: the arrival of pathogen-directed anti-infectives. J Med Microbiol 62(Pt 10):1506–1516CrossRefPubMed

38.

Wang IN, Smith DL, Young R (2000) Holins: the protein clocks of bacteriophage infections. Annu Rev Microbiol 54:799–825CrossRefPubMed

39.

Zhang J et al (2014) Complete genomic sequence of the Vibrio alginolyticus lytic bacteriophage PVA1. Arch Virol 159(12):3447–3451CrossRefPubMed

40.

Han F et al (2014) The novel Shewanella putrefaciens-infecting bacteriophage Spp001: genome sequence and lytic enzymes. J Ind Microbiol Biotechnol 41(6):1017–1026CrossRefPubMed

41.

42.

Loessner MJ et al (1997) Three Bacillus cereus bacteriophage endolysins are unrelated but reveal high homology to cell wall hydrolases from different bacilli. J Bacteriol 179(9):2845–2851PubMedPubMedCentral

43.

Loessner MJ, Wendlinger G, Scherer S (1995) Heterogeneous endolysins in Listeria monocytogenes bacteriophages: a new class of enzymes and evidence for conserved holin genes within the siphoviral lysis cassettes. Mol Microbiol 16(6):1231–1241CrossRefPubMed

44.

Vaara M (1992) Agents that increase the permeability of the outer membrane. Microbiol Rev 56(3):395–411PubMedPubMedCentral

45.

Lim JA et al (2012) Characterization of endolysin from a Salmonella typhimurium-infecting bacteriophage SPN1S. Res Microbiol 163(3):233–241CrossRefPubMed

46.

Briers Y, Walmagh M, Lavigne R (2011) Use of bacteriophage endolysin EL188 and outer membrane permeabilizers against Pseudomonas aeruginosa. J Appl Microbiol 110(3):778–785CrossRefPubMed

47.

Yoong P et al (2004) Identification of a broadly active phage lytic enzyme with lethal activity against antibiotic-resistant Enterococcus faecalis and Enterococcus faecium. J Bacteriol 186(14):4808–4812CrossRefPubMedPubMedCentral

48.

Briers Y, Lavigne R (2015) Breaking barriers: expansion of the use of endolysins as novel antibacterials against Gram-negative bacteria. Future Microbiol 10(3):377–390CrossRefPubMed

Titel: The Vibrio parahaemolyticus-infecting bacteriophage qdvp001: genome sequence and endolysin with a modular structure
verfasst von: Weiyu Wang
Mengzhe Li
Hong Lin
Jingxue Wang
Xiangzhao Mao
Publikationsdatum: 04.07.2016
Verlag: Springer Vienna
Erschienen in: Archives of Virology / Ausgabe 10/2016
Print ISSN: 0304-8608
Elektronische ISSN: 1432-8798
DOI: https://doi.org/10.1007/s00705-016-2957-x

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