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30.04.2018 | Research Article

Performance Evaluation of a Dedicated Preclinical PET/CT System for the Assessment of Mineralization Process in a Mouse Model of Atherosclerosis

verfasst von: Guillaume Rucher, Lucie Cameliere, Jihene Fendri, Ahmed Abbas, Kevin Dupont, Said Kamel, Nicolas Delcroix, Axel Dupont, Ludovic Berger, Alain Manrique

Erschienen in: Molecular Imaging and Biology | Ausgabe 6/2018

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Abstract

Purpose

The purpose of this study was to assess the impact of positron emission tomography/X-ray computed tomography (PET/CT) acquisition and reconstruction parameters on the assessment of mineralization process in a mouse model of atherosclerosis.

Procedures

All experiments were performed on a dedicated preclinical PET/CT system. CT was evaluated using five acquisition configurations using both a tungsten wire phantom for in-plane resolution assessment and a bar pattern phantom for cross-plane resolution. Furthermore, the radiation dose of these acquisition configurations was calculated. The PET system was assessed using longitudinal line sources to determine the optimal reconstruction parameters by measuring central resolution and its coefficient of variation. An in vivo PET study was performed using uremic ApoE^−/−, non-uremic ApoE^−/−, and control mice to evaluate optimal PET reconstruction parameters for the detection of sodium [¹⁸F]fluoride (Na[¹⁸F]F) aortic uptake and for quantitative measurement of Na[¹⁸F]F bone influx (Ki) with a Patlak analysis.

Results

For CT, the use of 1 × 1 and 2 × 2 binning detector mode increased both in-plane and cross-plane resolution. However, resolution improvement (163 to 62 μm for in-plane resolution) was associated with an important radiation dose increase (1.67 to 32.78 Gy). With PET, 3D-ordered subset expectation maximization (3D-OSEM) algorithm increased the central resolution compared to filtered back projection (1.42 ± 0.35 mm vs. 1.91 ± 0.08, p < 0.001). The use of 3D-OSEM with eight iterations and a zoom factor 2 yielded optimal PET resolution for preclinical study (FWHM = 0.98 mm). These PET reconstruction parameters allowed the detection of Na[¹⁸F]F aortic uptake in 3/14 ApoE^−/− mice and demonstrated a decreased Ki in uremic ApoE^−/− compared to non-uremic ApoE^−/− and control mice (p < 0.006).

Conclusions

Optimizing reconstruction parameters significantly impacted on the assessment of mineralization process in a preclinical model of accelerated atherosclerosis using Na[¹⁸F]F PET. In addition, improving the CT resolution was associated with a dramatic radiation dose increase.

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Titel: Performance Evaluation of a Dedicated Preclinical PET/CT System for the Assessment of Mineralization Process in a Mouse Model of Atherosclerosis
verfasst von: Guillaume Rucher
Lucie Cameliere
Jihene Fendri
Ahmed Abbas
Kevin Dupont
Said Kamel
Nicolas Delcroix
Axel Dupont
Ludovic Berger
Alain Manrique
Publikationsdatum: 30.04.2018
Verlag: Springer International Publishing
Erschienen in: Molecular Imaging and Biology / Ausgabe 6/2018
Print ISSN: 1536-1632
Elektronische ISSN: 1860-2002
DOI: https://doi.org/10.1007/s11307-018-1202-2

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Performance Evaluation of a Dedicated Preclinical PET/CT System for the Assessment of Mineralization Process in a Mouse Model of Atherosclerosis