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Journal of Anesthesia
Erschienen in: Journal of Anesthesia 2/2010

01.04.2010 | Original Article

Temporal profiles of aquaporin 4 expression and astrocyte response in the process of brain damage in fat embolism model in rats

verfasst von: Toru Gohara, Kazuyoshi Ishida, Kazuhiko Nakakimura, Mitsuyoshi Yoshida, Shiro Fukuda, Mishiya Matsumoto, Takefumi Sakabe

Erschienen in: Journal of Anesthesia | Ausgabe 2/2010

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Abstract

Purpose

Fat embolism syndrome is a serious complication observed after trauma, orthopedic surgery, and cardiac surgery. We investigated brain damage in relationship to temporal profiles of water channel aquaporin 4 (AQP4) and astrocyte response to fat embolism in rats.

Methods

Triolein (2 μl) was injected into the right internal carotid artery in rats. Neurological outcome (score: range, 0–5 = no deficit–dead), brain water content, histopathology, and immunohistochemistry for AQP4 and glial fibrillary acidic protein (GFAP) were evaluated at 2 h (2 h group, n = 12), 24 h (24 h group, n = 12), and 72 h (72 h group, n = 12) after triolein injection. Saline was injected in the control (C) group (n = 12).

Results

Neurological deficit score (median score of 2) and brain water content (mean value, 86.2%) increased significantly at 2 h with no progressive increase over 72 h. Damaged tissues with shrunken and triangular-shaped neurons with vacuole degeneration in cytoplasm and halo formation were distributed mainly, but not exclusively, to the ipsilateral hemisphere and were associated with increase in infiltration of inflammatory cells during the time course. Increases in immunostaining for AQP4 and GFAP were observed in the peri-affected region but not in the core. Reactive astrocytes with hypertrophy and dendrite elongation were detected at 72 h in the peri-affected region.

Conclusion

The results suggest that brain damage with edema is induced very rapidly after triolein injection in association with increase in AQP4 expression and GFAP in the peri-affected region.
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Metadaten
Titel
Temporal profiles of aquaporin 4 expression and astrocyte response in the process of brain damage in fat embolism model in rats
verfasst von
Toru Gohara
Kazuyoshi Ishida
Kazuhiko Nakakimura
Mitsuyoshi Yoshida
Shiro Fukuda
Mishiya Matsumoto
Takefumi Sakabe
Publikationsdatum
01.04.2010
Verlag
Springer Japan
Erschienen in
Journal of Anesthesia / Ausgabe 2/2010
Print ISSN: 0913-8668
Elektronische ISSN: 1438-8359
DOI
https://doi.org/10.1007/s00540-009-0831-7

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