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Journal of Artificial Organs

Erschienen in:

01.03.2015 | Review

Tissue engineering of oral mucosa: a shared concept with skin

verfasst von: Beste Kinikoglu, Odile Damour, Vasif Hasirci

Erschienen in: Journal of Artificial Organs | Ausgabe 1/2015

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Abstract

Tissue-engineered oral mucosa, in the form of epithelial cell sheets or full-thickness oral mucosa equivalents, is a potential solution for many patients with congenital defects or with tissue loss due to diseases or tumor excision following a craniofacial cancer diagnosis. In the laboratory, it further serves as an in vitro model, alternative to in vivo testing of oral care products, and provides insight into the behavior of the oral mucosal cells in healthy and pathological tissues. This review covers the old and new generation scaffold types and materials used in oral mucosa engineering; discusses similarities and differences between oral mucosa and skin, the methods developed to reconstruct oral mucosal defects; and ends with future perspectives on oral mucosa engineering.

Feinberg SE, Aghaloo TL, Cunningham LL Jr. Role of tissue engineering in oral and maxillofacial reconstruction: findings of the 2005 AAOMS Research Summit. J Oral Maxillofac Surg. 2005;63:1418–25.PubMed

MacNeil S. Progress and opportunities for tissue-engineered skin. Nature. 2007;445:874–80.PubMed

Ueda M, Tohnai I, Nakai H. Tissue engineering research in oral implant surgery. Artif Organs. 2001;25:164–71.PubMed

Lauer G. Fundamentals of tissue engineering and regenerative medicine. Berlin Heidelberg: Springer; 2009.

Will J, Melcher R, Treul C, Travitzky N, Kneser U, Polykandriotis E, et al. Porous ceramic bone scaffolds for vascularized bone tissue regeneration. J Mater Sci Mater Med. 2008;19:2781–90.PubMed

Squier CA, Kremer MJ. Biology of oral mucosa and esophagus. J Natl Cancer Inst Monogr. 2001;29:7–15.PubMed

Winning TA, Townsend GC. Oral mucosal embryology and histology. Clin Dermatol. 2000;18:499–511.PubMed

Izumi K, Feinberg SE, Iida A, Yoshizawa M. Intraoral grafting of an ex vivo produced oral mucosa equivalent: a preliminary report. Int J Oral Maxillofac Surg. 2003;32:188–97.PubMed

Squier CA. The permeability of oral mucosa. Crit Rev Oral Biol Med. 1991;2:13–32.PubMed

10.

Raghoebar GM, Tomson AM, Scholma J, Blaauw EH, Witjes MJ, Vissink A. Use of cultured mucosal grafts to cover defects caused by vestibuloplasty: an in vivo study. J Oral Maxillofac Surg. 1995;53:872–8.PubMed

11.

Okano T, Yamada N, Sakai H, Sakurai Y. A novel recovery system for cultured cells using plasma-treated polystyrene dishes grafted with poly(N-isopropylacrylamide). J Biomed Mater Res. 1993;27:1243–51.PubMed

12.

Nakamura T, Endo K, Cooper LJ, Fullwood NJ, Tanifuji N, Tsuzuki M, et al. The successful culture and autologous transplantation of rabbit oral mucosal epithelial cells on amniotic membrane. Invest Ophthalmol Vis Sci. 2003;44:106–16.PubMed

13.

Imaizumi F, Asahina I, Moriyama T, Ishii M, Omura K. Cultured mucosal cell sheet with a double layer of keratinocytes and fibroblasts on a collagen membrane. Tissue Eng. 2004;10:657–64.PubMed

14.

Yamada N, Okano T, Sakai H, Karikusa F, Sawasaki Y, Sakurai Y. Thermo-responsive polymeric surfaces; control of attachment and detachment of cultured cells. Makromol Chem Rapid Commun. 1990;11:571–6.

15.

Yang YJ, Yamato M, Kohno C, Nishimoto A, Sekine H, Fukai F, et al. Cell sheet engineering: recreating tissues without biodegradable scaffolds. Biomaterials. 2005;26:6415–22.PubMed

16.

Murakami D, Yamato M, Nishida K, Ohki T, Takagi R, Yang J, et al. Fabrication of transplantable human oral mucosal epithelial cell sheets using temperature-responsive culture inserts without feeder layer cells. J Artif Organs. 2006;9:185–9.PubMed

17.

Ueda M. Formation of epithelial sheets by serially cultivated human mucosal cells and their applications as a graft material. Nagoya J Med Sci. 1995;58:13–28.PubMed

18.

Langdon J, Williams DM, Navsaria H, Leigh IM. Autologous keratinocyte grafting: a new technique for intra-oral reconstruction. Br Dent J. 1991;171:87–90.PubMed

19.

Bodner L, Grossman N. Autologous cultured mucosal graft to cover large intraoral mucosal defects: a clinical study. J Oral Maxillofac Surg. 2003;61:169–73.PubMed

20.

Nishida K, Yamato M, Hayashida Y, Watanabe K, Yamamoto K, Adachi E, et al. Corneal reconstruction with tissue-engineered cell sheets composed of autologous oral mucosal epithelium. N Engl J Med. 2004;351:1187–96.PubMed

21.

Inatomi T, Nakamura T, Koizumi N, Sotozono C, Kinoshita S. Current progress and challenges in ocular surface reconstruction using cultivated epithelial sheet transplantation. Med J Malaysia 2008;63:Suppl A:42.

22.

Satake Y, Higa K, Tsubota K, Shimazaki J. Long-term outcome of cultivated oral mucosal epithelial sheet transplantation in treatment of total limbal stem cell deficiency. Ophthalmology. 2011;118:1524–30.PubMed

23.

Burillon C, Huot L, Justin V, Nataf S, Chapuis F, Decullier E, et al. Cultured autologous oral mucosal epithelial cell sheet (CAOMECS) transplantation for the treatment of corneal limbal stem cell deficiency. Invest Ophthalmol Vis Sci. 2012;53:1325–31.PubMed

24.

Sotozono C, Inatomi T, Nakamura T, Koizumi N, Yokoi N, Ueta M, et al. Visual improvement after cultivated oral mucosal epithelial transplantation. Ophthalmology. 2013;120:193–200.PubMed

25.

Ohki T, Yamato M, Murakami D, Takagi R, Yang J, Namiki H, et al. Treatment of oesophageal ulcerations using endoscopic transplantation of tissue-engineered autologous oral mucosal epithelial cell sheets in a canine model. Gut. 2006;55:1704–10.PubMedCentralPubMed

26.

Takagi R, Murakami D, Kondo M, Ohki T, Sasaki R, Mizutani M, et al. Fabrication of human oral mucosal epithelial cell sheets for treatment of esophageal ulceration by endoscopic submucosal dissection. Gastrointest Endosc. 2010;72:1253–9.PubMed

27.

Watanabe E, Yamato M, Shiroyanagi Y, Tanabe K, Okano T. Bladder augmentation using tissue-engineered autologous oral mucosal epithelial cell sheets grafted on demucosalized gastric flaps. Transplantation. 2011;91:700–6.PubMed

28.

Gallico GG, O’Conner NE. Engineering a skin replacement. Tissue Eng. 1995;1:231–40.PubMed

29.

Dongari-Bagtzoglou A, Kashleva H. Development of a highly reproducible three-dimensional organotypic model of the oral mucosa. Nat Protoc. 2006;1:2012–8.PubMedCentralPubMed

30.

Yoshizawa M, Feinberg SE, Marcelo CL, Elner VM. Ex vivo produced human conjunctiva and oral mucosa equivalents grown in a serum-free culture system. J Oral Maxillofac Surg. 2004;62:980–8.PubMed

31.

Nakanishi Y, Izumi K, Yoshizawa M, Saito C, Kawano Y, Maeda T. The expression and production of vascular endothelial growth factor in oral mucosa equivalents. Int J Oral Maxillofac Surg. 2007;36:928–33.PubMed

32.

Xiong X, Zhao Y, Zhang W, Xie W, He S. In vitro engineering of a palatal mucosa equivalent with acellular porcine dermal matrix. J Biomed Mater Res A. 2008;86:544–51.PubMed

33.

Tra WM, van Neck JW, Hovius SE, van Osch GJ, Perez-Amodio S. Characterization of a three-dimensional mucosal equivalent: similarities and differences with native oral mucosa. Cells Tissues Organs. 2012;195:185–96.PubMed

34.

Alaminos M, Garzón I, Sánchez-Quevedo MC, Moreu G, González-Andrades M, Fernández-Montoya A, et al. Time-course study of histological and genetic patterns of differentiation in human engineered oral mucosa. J Tissue Eng Regen Med. 2007;1:350–9.PubMed

35.

Luitaud C, Laflamme C, Semlali A, Saidi S, Grenier G, Zakrzewski A, et al. Development of an engineering autologous palatal mucosa-like tissue for potential clinical applications. J Biomed Mater Res B Appl Biomater. 2007;83:554–61.PubMed

36.

Moharamzadeh K, Brook IM, Van Noort R, Scutt AM, Smith KG, Thornhill MH. Development, optimization and characterization of a full-thickness tissue engineered human oral mucosal model for biological assessment of dental biomaterials. J Mater Sci Mater Med. 2008;19:1793–801.PubMed

37.

Kinikoglu B, Auxenfans C, Pierrillas P, Justin V, Breton P, Burillon C, et al. Reconstruction of a full-thickness collagen-based human oral mucosal equivalent. Biomaterials. 2009;30:6418–25.PubMed

38.

Chapple CR, Macneil S. Tissue engineered oral mucosa for urethroplasty: past experience and future directions. J Urol. 2012;187:1533–4.PubMed

39.

Xie M, Xu Y, Song L, Wang J, Lv X, Zhang Y. Tissue-engineered buccal mucosa using silk fibroin matrices for urethral reconstruction in a canine model. J Surg Res 2013; pii: S0022-4804(13)02134-3.

40.

Lucier RN, Etienne O, Ferreira S, Garlick JA, Kugel G, Egles C. Soft-tissue alterations following exposure to tooth-whitening agents. J Periodontol. 2013;84:513–9.PubMed

41.

Moharamzadeh K, Franklin KL, Brook IM, van Noort R. Biologic assessment of antiseptic mouthwashes using a three-dimensional human oral mucosal model. J Periodontol. 2009;80:769–75.PubMed

42.

Kinikoglu B, Rovere MR, Haftek M, Hasirci V, Damour O. Influence of the mesenchymal cell source on oral epithelial development. J Tissue Eng Regen Med. 2012;6:245–52.PubMed

43.

Bucchieri F, Fucarino A, Marino Gammazza A, Pitruzzella A, Marciano V, Paderni C, et al. Medium-term culture of normal human oral mucosa: a novel three-dimensional model to study the effectiveness of drugs administration. Curr Pharm Des 2012;18:5421-30.

44.

Chai WL, Brook IM, Palmquist A, van Noort R, Moharamzadeh K. The biological seal of the implant-soft tissue interface evaluated in a tissue-engineered oral mucosal model. J R Soc Interface. 2012;9:3528–38.PubMedCentralPubMed

45.

Colley HE, Hearnden V, Jones AV, Weinreb PH, Violette SM, Macneil S, et al. Development of tissue-engineered models of oral dysplasia and early invasive oral squamous cell carcinoma. Br J Cancer. 2011;105:1582–92.PubMedCentralPubMed

46.

Yadev NP, Murdoch C, Saville SP, Thornhill MH. Evaluation of tissue engineered models of the oral mucosa to investigate oral candidiasis. Microb Pathog. 2011;50:278–85.PubMed

47.

Colley HE, Eves PC, Pinnock A, Thornhill MH, Murdoch C. Tissue-engineered oral mucosa to study radiotherapy-induced oral mucositis. Int J Radiat Biol. 2013;89:907–14.PubMed

48.

Cho KH, Ahn HT, Park KC, Chung JH, Kim SW, Sung MW, et al. Reconstruction of human hard-palate mucosal epithelium on deepidermized dermis. J Dermatol Sci. 2000;22:117–24.PubMed

49.

Ophof R, van Rheden RE, Von den Hoffa JW, Schalkwijk J, Kuijpers-Jagtman AM. Oral keratinocytes cultured on dermal matrices form a mucosa-like tissue. Biomaterials. 2002;23:3741–8.PubMed

50.

Bhargava S, Chapple CR, Bullock AJ, Layton C, MacNeil S. Tissue engineered buccal mucosa for substitution urethroplasty. BJU Int. 2004;93:807–11.PubMed

51.

Iida T, Takami Y, Yamaguchi R, Shimazaki S, Harii K. Development of a tissue-engineered human oral mucosa equivalent based on an acellular allogeneic dermal matrix: a preliminary report of clinical application to burn wounds. Scand J Plast Reconstr Surg Hand Surg. 2005;39:138–46.PubMed

52.

Izumi K, Takacs G, Terashi H, Feinberg SE. Ex vivo development of a composite human oral mucosal equivalent. J Oral Maxillofac Surg. 1999;57:571–7.PubMed

53.

Izumi K, Terashi H, Marcelo CL, Feinberg SE. Development and characterization of a tissue-engineered human oral mucosa equivalent produced in a serum-free culture system. J Dent Res. 2000;79:798–805.PubMed

54.

Yoshizawa M, Koyama T, Kojima T, Kato H, Ono Y, Saito C. Keratinocytes of tissue-engineered human oral mucosa promote re-epithelialization after intraoral grafting in athymic mice. J Oral Maxillofac Surg. 2012;70:1199–214.PubMed

55.

Hildebrand HC, Hakkinen L, Wiebe CB, Larjava HS. Characterization of organotypic keratinocyte cultures on deepithelialized bovine tongue mucosa. Histol Histopathol. 2002;17:151–63.PubMed

56.

Dang JM, Leong KW. Natural polymers for gene delivery and tissue engineering. Adv Drug Deliv Rev. 2006;58:487–99.PubMed

57.

Yannas IV, Burke JF. Design of an artificial skin. I. Basic design principles. J Biomed Mater Res. 1980;14:65–81.PubMed

58.

Yannas IV, Burke JF, Gordon PL, Huang C, Rubenstein RH. Design of an artificial skin. II. Control of chemical composition. J Biomed Mater Res. 1980;14:107–32.PubMed

59.

Glowacki J, Mizuno S. Collagen scaffolds for tissue engineering. Biopolymers. 2008;89:338–44.PubMed

60.

Moharamzadeh K, Brook IM, van Noort R, Scutt AM, Thornhill MH. Tissue-engineered oral mucosa: a review of the scientific literature. J Dent Res. 2007;86:115–24.PubMed

61.

Claveau I, Mostefaoui Y, Rouabhia M. Basement membrane protein and matrix metalloproteinase deregulation in engineered human oral mucosa following infection with Candida albicans. Matrix Biol. 2004;23:477–86.PubMed

62.

Tardif F, Goulet JP, Zakrazewski A, Chauvin P, Rouabhia M. Involvement of interleukin-18 in the inflammatory response against oropharyngeal candidiasis. Med Sci Monit. 2004;10:239–49.

63.

Kinikoglu B, Hemar J, Hasirci V, Breton P, Damour O. Feasibility of a porcine oral mucosa equivalent: a preclinical study. Artif Cell Blood Sub. 2012;40:271–4.

64.

Terada M, Izumi K, Ohnuki H, Saito T, Kato H, Yamamoto M, et al. Construction and characterization of a tissue-engineered oral mucosa equivalent based on a chitosan-fish scale collagen composite. J Biomed Mater Res B Appl Biomater. 2012;100:1792–802.PubMed

65.

Bustos RH, Suesca E, Millán D, González JM, Fontanilla MR. Real-time quantification of proteins secreted by artificial connective tissue made from uni- or multidirectional collagen I scaffolds and oral mucosa fibroblasts. Anal Chem. 2014;86:2421–8.PubMed

66.

Peña I, Junquera LM, Meana A, García E, García V, De Vicente JC. In vitro engineering of complete autologous oral mucosa equivalents: characterization of a novel scaffold. J Periodontal Res. 2010;45:375–80.PubMed

67.

San Martin S, Alaminos M, Zorn TM, Sánchez-Quevedo MC, Garzón I, Rodriguez IA et al. The effects of fibrin and fibrin-agarose on the extracellular matrix profile of bioengineered oral mucosa. J Tissue Eng Regen Med 2013;7:10-9.

68.

Golinski PA, Gröger S, Herrmann JM, Bernd A, Meyle J. Oral mucosa model based on a collagen-elastin matrix. J Periodontal Res. 2011;46:704–11.PubMed

69.

Gunatillake PA, Adhikari R. Biodegradable synthetic polymers for tissue engineering. Eur Cell Mater. 2003;5:1–16.PubMed

70.

Rodríguez-Cabello JC, Prieto S, Reguera J, Arias FJ, Ribeiro A. Biofunctional design of elastin-like polymers for advanced applications in nanobiotechnology. J Biomater Sci Polymer Edn. 2007;18:269–86.

71.

Rodríguez-Cabello JC, Martín L, Alonso M, Arias FJ, Testera AM. “Recombinamers” as advanced materials for the post-oil age. Polymer. 2009;50:5159–69.

72.

Chilkoti A, Christensen T, MacKay JA. Stimulus responsive elastin biopolymers: applications in medicine and biotechnology. Curr Opin Chem Biol. 2006;10:652–7.PubMedCentralPubMed

73.

Ozturk N, Girotti A, Kose GT, Rodríguez-Cabello JC, Hasirci V. Dynamic cell culturing and its application to micropatterned, elastin-like protein-modified poly(N-isopropylacrylamide) scaffolds. Biomaterials. 2009;30:5417–26.PubMed

74.

Costa RR, Custódio CA, Arias FJ, Rodríguez-Cabello JC, Mano JF. Layer-by-layer assembly of chitosan and recombinant biopolymers into biomimetic coatings with multiple stimuli-responsive properties. Small. 2011;7:2640–9.PubMed

75.

Minato A, Ise H, Goto M, Akaike T. Cardiac differentiation of embryonic stem cells by substrate immobilization of insulin-like growth factor binding protein 4 with elastin-like polypeptides. Biomaterials. 2012;33:515–23.PubMed

76.

Ciofani G, Genchi GG, Liakos I, Athanassiou A, Mattoli V, Bandiera A. Human recombinant elastin-like protein coatings for muscle cell proliferation and differentiation. Acta Biomater. 2013;9:5111–21.PubMed

77.

Huang L, McMillan RA, Apkarian RP, Pourdeyhimi B, Conticello VP, Chaikof EL. Generation of synthetic elastin-mimetic small diameter fibers and fiber networks. Macromolecules. 2000;33:2989–97.

78.

Martínez-Osorio H, Juárez-Campo M, Diebold Y, Girotti A, Alonso M, Arias FJ, et al. Genetically engineered elastin-like polymer as a substratum to culture cells from the ocular surface. Curr Eye Res. 2009;34:48–56.PubMed

79.

Costa RR, Testera AM, Arias FJ, Rodríguez-Cabello JC, Mano JF. Layer-by-layer film growth using polysaccharides and recombinant polypeptides: a combinatorial approach. J Phys Chem B. 2013;117:6839–48.PubMed

80.

Betre H, Ong SR, Guilak F, Chilkoti A, Fermor B, Setton LA. Chondrocytic differentiation of human adipose-derived adult stem cells in elastin-like polypeptide. Biomaterials. 2006;27:91–9.PubMed

81.

Martín L, Alonso M, Girotti A, Arias FJ, Rodríguez-Cabello JC. Synthesis and characterization of macroporous thermosensitive hydrogels from recombinant elastin-like polymers. Biomacromolecules. 2009;10:3015–22.PubMed

82.

Nettles DL, Haider MA, Chilkoti A, Setton LA. Neural network analysis identifies scaffold properties necessary for in vitro chondrogenesis in elastin-like polypeptide biopolymer scaffolds. Tissue Eng Part A. 2010;16:11–20.PubMedCentralPubMed

83.

Asai D, Xu D, Liu W, Garcia Quiroz F, Callahan DJ, Zalutsky MR, et al. Protein polymer hydrogels by in situ, rapid and reversible self-gelation. Biomaterials 2012;33:5451-8.

84.

Urry DW, Pattanaik A, Xu J, Woods TC, McPherson DT, Parker TM. Elastic protein-based polymers in soft tissue augmentation and generation. In: Polymers for tissue engineering. Brill Academic Publishers, The Netherlands (1998).

85.

Adams SB Jr, Shamji MF, Nettles DL, Hwang P, Setton LA. Sustained release of antibiotics from injectable and thermally responsive polypeptide depots. J Biomed Mater Res B Appl Biomater. 2009;90:67–74.PubMedCentralPubMed

86.

Garcia Y, Hemantkumar N, Collighan R, Griffin M, Rodriguez-Cabello JC, Pandit A. In vitro characterization of a collagen scaffold enzymatically cross-linked with a tailored elastin-like polymer. Tissue Eng Part A. 2009;15:887–99.PubMed

87.

Kinikoglu B, Rodríguez-Cabello JC, Damour O, Hasirci V. A smart bilayer scaffold of elastin-like recombinamer and collagen for soft tissue engineering. J Mater Sci Mater Med. 2011;22:1541–54.PubMed

88.

Kinikoglu B, Rodríguez-Cabello JC, Damour O, Hasirci V. The influence of elastin-like recombinant polymer on the self-renewing potential of a 3D tissue equivalent derived from human lamina propria fibroblasts and oral epithelial cells. Biomaterials. 2011;32:5756–64.PubMed

89.

Buckley CT, O’Kelly KU. Regular scaffold fabrication techniques for investigations in tissue engineering, Trinity Centre for Bioengineering & National Centre for Biomedical Engineering Science (2004).

90.

Weigel T, Schinkel G, Lendlein A. Design and preparation of polymeric scaffolds for tissue engineering. Expert Rev Med Devices. 2006;3:835–51.PubMed

91.

Chen G, Ushida T, Tateishi T. Scaffold design for tissue engineering. Macromol Biosci. 2002;2:67–77.

92.

Freyman TM, Yannas IV, Gibson LJ. Cellular materials as porous scaffolds for tissue engineering. Progr Mater Sci. 2001;46:273–82.

93.

Faraj KA, van Kuppevelt TH, Daamen WF. Construction of collagen scaffolds that mimic the three-dimensional architecture of specific tissues. Tissue Eng. 2007;13:2387–94.PubMed

94.

Madaghiele M, Sannino A, Yannas IV, Spector M. Collagen-based matrices with axially oriented pores. J Biomed Mater Res Part A. 2008;85A:757–67.

95.

Nisbet DR, Forsythe JS, Shen W, Finkelstein DI, Horne MK. Review paper: a review of the cellular response on electrospun nanofibers for tissue engineering. J Biomater Appl. 2009;24:7–29.PubMed

96.

Matthews JA, Wnek GE, Simpson DG, Bowlin GL. Electrospinning of collagen nanofibers. Biomacromolecules. 2002;3:232–8.PubMed

97.

Tuzlakoglu K, Reis RL. Biodegradable polymeric fiber structures in tissue engineering. Tissue Eng Part B Rev. 2009;15:17–27.PubMed

98.

Powell HM, Supp DM, Boyce ST. Influence of electrospun collagen on wound contraction of engineered skin substitutes. Biomaterials. 2008;29:834–43.PubMed

99.

Rho KS, Jeong L, Lee G, Seo BM, Park YJ, Hong SD, et al. Electrospinning of collagen nanofibers: effects on the behavior of normal human keratinocytes and early-stage wound healing. Biomaterials. 2006;27:1452–61.PubMed

100.

Noh HK, Lee SW, Kim JM, Oh JE, Kim KH, Chung CP, et al. Electrospinning of chitin nanofibers: degradation behavior and cellular response to normal human keratinocytes and fibroblasts. Biomaterials. 2006;27:3934–44.PubMed

101.

Blackwood KA, McKean R, Canton I, Freeman CO, Franklin KL, Cole D, et al. Development of biodegradable electrospun scaffolds for dermal replacement. Biomaterials. 2008;29:3091–104.PubMed

102.

Yeo IS, Oh JE, Jeong L, Lee TS, Lee SJ, Park WH, et al. Collagen-based biomimetic nanofibrous scaffolds: preparation and characterization of collagen/silk fibroin bicomponent nanofibrous structures. Biomacromolecules. 2008;9:1106–16.PubMed

103.

Zhou Y, Yang D, Chen X, Xu Q, Lu F, Nie J. Electrospun water-soluble carboxyethyl chitosan/poly(vinyl alcohol) nanofibrous membrane as potential wound dressing for skin regeneration. Biomacromolecules. 2008;9:349–54.PubMed

104.

Duan B, Wu L, Yuan X, Hu Z, Li X, Zhang Y, et al. Hybrid nanofibrous membranes of PLGA/chitosan fabricated via an electrospinning array. J Biomed Mater Res A. 2007;83:868–78.PubMed

105.

Kim G, Kim W. Highly porous 3D nanofiber scaffold using an electrospinning technique. J Biomed Mater Res B Appl Biomater. 2007;81:104–10.PubMed

106.

Black AF, Hudon V, Damour O, Germain L, Auger FA. A novel approach for studying angiogenesis: a human skin equivalent with a capillary-like network. Cell Biol Toxicol. 1999;15:81–90.PubMed

107.

Wake MC, Patrick CW Jr, Mikos AG. Pore morphology effects on the fibrovascular tissue growth in porous polymer substrates. Cell Transplant. 1994;3:339–43.PubMed

108.

Hosseinkhani H, Hosseinkhani M, Khademhosseini A, Kobayashi H, Tabata Y. Enhanced angiogenesis through controlled release of basic fibroblast growth factor from peptide amphiphile for tissue regeneration. Biomaterials. 2006;27:5836–44.PubMed

109.

Sonis ST. The pathobiology of mucositis. Nat Rev Cancer. 2004;4:277–84.PubMed

Titel: Tissue engineering of oral mucosa: a shared concept with skin
verfasst von: Beste Kinikoglu
Odile Damour
Vasif Hasirci
Publikationsdatum: 01.03.2015
Verlag: Springer Japan
Erschienen in: Journal of Artificial Organs / Ausgabe 1/2015
Print ISSN: 1434-7229
Elektronische ISSN: 1619-0904
DOI: https://doi.org/10.1007/s10047-014-0798-5

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Inhalte des Webinars zur S1-Leitlinie „Empfehlungen zur Therapie der akuten Appendizitis bei Erwachsenen“ sind die Darstellung des Projektes und des Erstellungswegs zur S1-Leitlinie, die Erläuterung der klinischen Relevanz der Klassifikation EAES 2015, die wissenschaftliche Begründung der wichtigsten Empfehlungen und die Darstellung stadiengerechter Therapieoptionen.

Dr. med. Mihailo Andric

Live-Webinar "Urologie und Sexualmedizin in der Praxis"

Springer Medizin

Tissue engineering of oral mucosa: a shared concept with skin

Abstract

Neu im Fachgebiet Chirurgie

Häusliche Gewalt in der orthopädischen Notaufnahme oft nicht erkannt

Fehlerkultur in der Medizin – Offenheit zählt!

Mehr Frauen im OP – weniger postoperative Komplikationen

TAVI versus Klappenchirurgie: Neue Vergleichsstudie sorgt für Erstaunen

Update Chirurgie

Aktuelle BDC Leitlinien-Webinare

S3-Leitlinie „Diagnostik und Therapie des Karpaltunnelsyndroms“

S2e-Leitlinie „Distale Radiusfraktur“

S1-Leitlinie „Empfehlungen zur Therapie der akuten Appendizitis bei Erwachsenen“

Live-Webinar "Urologie und Sexualmedizin in der Praxis"

Springer Medizin

Abstract

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In situ observation and enhancement of leaflet tissue formation in bioprosthetic “biovalve”

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